Job ID = 6527481 SRX = SRX026858 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:24:33 prefetch.2.10.7: 1) Downloading 'SRR065756'... 2020-06-29T12:24:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:28:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:28:05 prefetch.2.10.7: 1) 'SRR065756' was downloaded successfully Read 27229441 spots for SRR065756/SRR065756.sra Written 27229441 spots for SRR065756/SRR065756.sra 2020-06-29T12:29:32 prefetch.2.10.7: 1) Downloading 'SRR065757'... 2020-06-29T12:29:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:32:01 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:32:01 prefetch.2.10.7: 1) 'SRR065757' was downloaded successfully Read 27222800 spots for SRR065757/SRR065757.sra Written 27222800 spots for SRR065757/SRR065757.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:54 54452241 reads; of these: 54452241 (100.00%) were unpaired; of these: 2174645 (3.99%) aligned 0 times 35657153 (65.48%) aligned exactly 1 time 16620443 (30.52%) aligned >1 times 96.01% overall alignment rate Time searching: 00:15:54 Overall time: 00:15:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 17094771 / 52277596 = 0.3270 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:08:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:08:19: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:08:19: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:08:24: 1000000 INFO @ Mon, 29 Jun 2020 22:08:29: 2000000 INFO @ Mon, 29 Jun 2020 22:08:34: 3000000 INFO @ Mon, 29 Jun 2020 22:08:39: 4000000 INFO @ Mon, 29 Jun 2020 22:08:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:08:49: 6000000 INFO @ Mon, 29 Jun 2020 22:08:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:08:49: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:08:49: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:08:54: 7000000 INFO @ Mon, 29 Jun 2020 22:08:55: 1000000 INFO @ Mon, 29 Jun 2020 22:09:00: 8000000 INFO @ Mon, 29 Jun 2020 22:09:00: 2000000 INFO @ Mon, 29 Jun 2020 22:09:05: 9000000 INFO @ Mon, 29 Jun 2020 22:09:05: 3000000 INFO @ Mon, 29 Jun 2020 22:09:11: 10000000 INFO @ Mon, 29 Jun 2020 22:09:11: 4000000 INFO @ Mon, 29 Jun 2020 22:09:16: 11000000 INFO @ Mon, 29 Jun 2020 22:09:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:09:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:09:19: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:09:19: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:09:22: 12000000 INFO @ Mon, 29 Jun 2020 22:09:22: 6000000 INFO @ Mon, 29 Jun 2020 22:09:25: 1000000 INFO @ Mon, 29 Jun 2020 22:09:28: 13000000 INFO @ Mon, 29 Jun 2020 22:09:28: 7000000 INFO @ Mon, 29 Jun 2020 22:09:31: 2000000 INFO @ Mon, 29 Jun 2020 22:09:34: 14000000 INFO @ Mon, 29 Jun 2020 22:09:34: 8000000 INFO @ Mon, 29 Jun 2020 22:09:36: 3000000 INFO @ Mon, 29 Jun 2020 22:09:39: 15000000 INFO @ Mon, 29 Jun 2020 22:09:40: 9000000 INFO @ Mon, 29 Jun 2020 22:09:42: 4000000 INFO @ Mon, 29 Jun 2020 22:09:45: 16000000 INFO @ Mon, 29 Jun 2020 22:09:45: 10000000 INFO @ Mon, 29 Jun 2020 22:09:48: 5000000 INFO @ Mon, 29 Jun 2020 22:09:51: 17000000 INFO @ Mon, 29 Jun 2020 22:09:51: 11000000 INFO @ Mon, 29 Jun 2020 22:09:54: 6000000 INFO @ Mon, 29 Jun 2020 22:09:57: 18000000 INFO @ Mon, 29 Jun 2020 22:09:57: 12000000 INFO @ Mon, 29 Jun 2020 22:09:59: 7000000 INFO @ Mon, 29 Jun 2020 22:10:03: 19000000 INFO @ Mon, 29 Jun 2020 22:10:03: 13000000 INFO @ Mon, 29 Jun 2020 22:10:05: 8000000 INFO @ Mon, 29 Jun 2020 22:10:09: 20000000 INFO @ Mon, 29 Jun 2020 22:10:09: 14000000 INFO @ Mon, 29 Jun 2020 22:10:10: 9000000 INFO @ Mon, 29 Jun 2020 22:10:14: 21000000 INFO @ Mon, 29 Jun 2020 22:10:14: 15000000 INFO @ Mon, 29 Jun 2020 22:10:16: 10000000 INFO @ Mon, 29 Jun 2020 22:10:20: 22000000 INFO @ Mon, 29 Jun 2020 22:10:20: 16000000 INFO @ Mon, 29 Jun 2020 22:10:22: 11000000 INFO @ Mon, 29 Jun 2020 22:10:26: 23000000 INFO @ Mon, 29 Jun 2020 22:10:26: 17000000 INFO @ Mon, 29 Jun 2020 22:10:27: 12000000 INFO @ Mon, 29 Jun 2020 22:10:32: 18000000 INFO @ Mon, 29 Jun 2020 22:10:32: 24000000 INFO @ Mon, 29 Jun 2020 22:10:33: 13000000 INFO @ Mon, 29 Jun 2020 22:10:37: 19000000 INFO @ Mon, 29 Jun 2020 22:10:37: 25000000 INFO @ Mon, 29 Jun 2020 22:10:39: 14000000 INFO @ Mon, 29 Jun 2020 22:10:43: 20000000 INFO @ Mon, 29 Jun 2020 22:10:43: 26000000 INFO @ Mon, 29 Jun 2020 22:10:44: 15000000 INFO @ Mon, 29 Jun 2020 22:10:48: 21000000 INFO @ Mon, 29 Jun 2020 22:10:49: 27000000 INFO @ Mon, 29 Jun 2020 22:10:50: 16000000 INFO @ Mon, 29 Jun 2020 22:10:54: 22000000 INFO @ Mon, 29 Jun 2020 22:10:54: 28000000 INFO @ Mon, 29 Jun 2020 22:10:55: 17000000 INFO @ Mon, 29 Jun 2020 22:11:00: 23000000 INFO @ Mon, 29 Jun 2020 22:11:00: 29000000 INFO @ Mon, 29 Jun 2020 22:11:01: 18000000 INFO @ Mon, 29 Jun 2020 22:11:06: 24000000 INFO @ Mon, 29 Jun 2020 22:11:06: 30000000 INFO @ Mon, 29 Jun 2020 22:11:06: 19000000 INFO @ Mon, 29 Jun 2020 22:11:11: 25000000 INFO @ Mon, 29 Jun 2020 22:11:12: 31000000 INFO @ Mon, 29 Jun 2020 22:11:12: 20000000 INFO @ Mon, 29 Jun 2020 22:11:17: 26000000 INFO @ Mon, 29 Jun 2020 22:11:17: 32000000 INFO @ Mon, 29 Jun 2020 22:11:18: 21000000 INFO @ Mon, 29 Jun 2020 22:11:22: 27000000 INFO @ Mon, 29 Jun 2020 22:11:23: 33000000 INFO @ Mon, 29 Jun 2020 22:11:23: 22000000 INFO @ Mon, 29 Jun 2020 22:11:28: 28000000 INFO @ Mon, 29 Jun 2020 22:11:28: 34000000 INFO @ Mon, 29 Jun 2020 22:11:29: 23000000 INFO @ Mon, 29 Jun 2020 22:11:34: 29000000 INFO @ Mon, 29 Jun 2020 22:11:34: 35000000 INFO @ Mon, 29 Jun 2020 22:11:34: 24000000 INFO @ Mon, 29 Jun 2020 22:11:35: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:11:35: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:11:35: #1 total tags in treatment: 35182825 INFO @ Mon, 29 Jun 2020 22:11:35: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:11:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:11:36: #1 tags after filtering in treatment: 35182825 INFO @ Mon, 29 Jun 2020 22:11:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:11:36: #1 finished! INFO @ Mon, 29 Jun 2020 22:11:36: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:11:38: #2 number of paired peaks: 34 WARNING @ Mon, 29 Jun 2020 22:11:38: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:11:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:11:39: 30000000 INFO @ Mon, 29 Jun 2020 22:11:40: 25000000 INFO @ Mon, 29 Jun 2020 22:11:45: 31000000 INFO @ Mon, 29 Jun 2020 22:11:45: 26000000 INFO @ Mon, 29 Jun 2020 22:11:50: 32000000 INFO @ Mon, 29 Jun 2020 22:11:51: 27000000 INFO @ Mon, 29 Jun 2020 22:11:56: 33000000 INFO @ Mon, 29 Jun 2020 22:11:56: 28000000 INFO @ Mon, 29 Jun 2020 22:12:01: 34000000 INFO @ Mon, 29 Jun 2020 22:12:02: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:12:07: 35000000 INFO @ Mon, 29 Jun 2020 22:12:07: 30000000 INFO @ Mon, 29 Jun 2020 22:12:08: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:12:08: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:12:08: #1 total tags in treatment: 35182825 INFO @ Mon, 29 Jun 2020 22:12:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:12:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:12:09: #1 tags after filtering in treatment: 35182825 INFO @ Mon, 29 Jun 2020 22:12:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:12:09: #1 finished! INFO @ Mon, 29 Jun 2020 22:12:09: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:12:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:12:11: #2 number of paired peaks: 34 WARNING @ Mon, 29 Jun 2020 22:12:11: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:12:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:12:13: 31000000 INFO @ Mon, 29 Jun 2020 22:12:18: 32000000 INFO @ Mon, 29 Jun 2020 22:12:23: 33000000 INFO @ Mon, 29 Jun 2020 22:12:28: 34000000 INFO @ Mon, 29 Jun 2020 22:12:33: 35000000 INFO @ Mon, 29 Jun 2020 22:12:35: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:12:35: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:12:35: #1 total tags in treatment: 35182825 INFO @ Mon, 29 Jun 2020 22:12:35: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:12:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:12:35: #1 tags after filtering in treatment: 35182825 INFO @ Mon, 29 Jun 2020 22:12:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:12:35: #1 finished! INFO @ Mon, 29 Jun 2020 22:12:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:12:37: #2 number of paired peaks: 34 WARNING @ Mon, 29 Jun 2020 22:12:37: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:12:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX026858/SRX026858.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。