Job ID = 2161249 sra ファイルのダウンロード中... Completed: 16417K bytes transferred in 3 seconds (39537K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 17821 0 17821 0 0 25611 0 --:--:-- --:--:-- --:--:-- 35289 100 35011 0 35011 0 0 50255 0 --:--:-- --:--:-- --:--:-- 69191 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 695909 spots for /home/okishinya/chipatlas/results/dm3/SRX013094/SRR030360.sra Written 695909 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:10 695909 reads; of these: 695909 (100.00%) were unpaired; of these: 115252 (16.56%) aligned 0 times 450041 (64.67%) aligned exactly 1 time 130616 (18.77%) aligned >1 times 83.44% overall alignment rate Time searching: 00:00:10 Overall time: 00:00:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 31197 / 580657 = 0.0537 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 21 Apr 2015 12:07:12: # Command line: callpeak -t SRX013094.bam -f BAM -g dm -n SRX013094.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX013094.05 # format = BAM # ChIP-seq file = ['SRX013094.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 12:07:12: # Command line: callpeak -t SRX013094.bam -f BAM -g dm -n SRX013094.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX013094.10 # format = BAM # ChIP-seq file = ['SRX013094.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 12:07:12: #1 read tag files... INFO @ Tue, 21 Apr 2015 12:07:12: #1 read tag files... INFO @ Tue, 21 Apr 2015 12:07:12: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 12:07:12: # Command line: callpeak -t SRX013094.bam -f BAM -g dm -n SRX013094.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX013094.20 # format = BAM # ChIP-seq file = ['SRX013094.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 12:07:12: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 12:07:12: #1 read tag files... INFO @ Tue, 21 Apr 2015 12:07:12: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 12:07:15: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 12:07:15: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 12:07:15: #1 total tags in treatment: 549460 INFO @ Tue, 21 Apr 2015 12:07:15: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 12:07:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 12:07:15: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 12:07:15: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 12:07:15: #1 total tags in treatment: 549460 INFO @ Tue, 21 Apr 2015 12:07:15: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 12:07:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 12:07:15: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 12:07:15: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 12:07:15: #1 total tags in treatment: 549460 INFO @ Tue, 21 Apr 2015 12:07:15: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 12:07:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 12:07:15: #1 tags after filtering in treatment: 549449 INFO @ Tue, 21 Apr 2015 12:07:15: #1 tags after filtering in treatment: 549449 INFO @ Tue, 21 Apr 2015 12:07:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 12:07:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 12:07:15: #1 finished! INFO @ Tue, 21 Apr 2015 12:07:15: #1 finished! INFO @ Tue, 21 Apr 2015 12:07:15: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 12:07:15: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 12:07:15: #1 tags after filtering in treatment: 549449 INFO @ Tue, 21 Apr 2015 12:07:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 12:07:15: #1 finished! INFO @ Tue, 21 Apr 2015 12:07:15: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 12:07:16: #2 number of paired peaks: 3068 INFO @ Tue, 21 Apr 2015 12:07:16: start model_add_line... INFO @ Tue, 21 Apr 2015 12:07:16: #2 number of paired peaks: 3068 INFO @ Tue, 21 Apr 2015 12:07:16: start model_add_line... INFO @ Tue, 21 Apr 2015 12:07:16: #2 number of paired peaks: 3068 INFO @ Tue, 21 Apr 2015 12:07:16: start model_add_line... INFO @ Tue, 21 Apr 2015 12:07:17: start X-correlation... INFO @ Tue, 21 Apr 2015 12:07:17: end of X-cor INFO @ Tue, 21 Apr 2015 12:07:17: #2 finished! INFO @ Tue, 21 Apr 2015 12:07:17: #2 predicted fragment length is 235 bps INFO @ Tue, 21 Apr 2015 12:07:17: #2 alternative fragment length(s) may be 235 bps INFO @ Tue, 21 Apr 2015 12:07:17: #2.2 Generate R script for model : SRX013094.05_model.r INFO @ Tue, 21 Apr 2015 12:07:17: #3 Call peaks... INFO @ Tue, 21 Apr 2015 12:07:17: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 12:07:17: start X-correlation... INFO @ Tue, 21 Apr 2015 12:07:17: end of X-cor INFO @ Tue, 21 Apr 2015 12:07:17: #2 finished! INFO @ Tue, 21 Apr 2015 12:07:17: #2 predicted fragment length is 235 bps INFO @ Tue, 21 Apr 2015 12:07:17: #2 alternative fragment length(s) may be 235 bps INFO @ Tue, 21 Apr 2015 12:07:17: #2.2 Generate R script for model : SRX013094.20_model.r INFO @ Tue, 21 Apr 2015 12:07:17: start X-correlation... INFO @ Tue, 21 Apr 2015 12:07:17: #3 Call peaks... INFO @ Tue, 21 Apr 2015 12:07:17: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 12:07:17: end of X-cor INFO @ Tue, 21 Apr 2015 12:07:17: #2 finished! INFO @ Tue, 21 Apr 2015 12:07:17: #2 predicted fragment length is 235 bps INFO @ Tue, 21 Apr 2015 12:07:17: #2 alternative fragment length(s) may be 235 bps INFO @ Tue, 21 Apr 2015 12:07:17: #2.2 Generate R script for model : SRX013094.10_model.r INFO @ Tue, 21 Apr 2015 12:07:17: #3 Call peaks... INFO @ Tue, 21 Apr 2015 12:07:17: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 12:07:20: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 12:07:20: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 12:07:20: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write output xls file... SRX013094.20_peaks.xls INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write peak in narrowPeak format file... SRX013094.20_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write summits bed file... SRX013094.20_summits.bed INFO @ Tue, 21 Apr 2015 12:07:23: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (4 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write output xls file... SRX013094.10_peaks.xls INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write peak in narrowPeak format file... SRX013094.10_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write summits bed file... SRX013094.10_summits.bed INFO @ Tue, 21 Apr 2015 12:07:23: Done! INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write output xls file... SRX013094.05_peaks.xls INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write peak in narrowPeak format file... SRX013094.05_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 12:07:23: #4 Write summits bed file... SRX013094.05_summits.bed INFO @ Tue, 21 Apr 2015 12:07:23: Done! pass1 - making usageList (2 chroms): 1 millis pass2 - checking and writing primary data (28 records, 4 fields): 1 millis CompletedMACS2peakCalling pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (101 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。