Job ID = 2161188 sra ファイルのダウンロード中... Completed: 110615K bytes transferred in 4 seconds (203960K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 34682 0 34682 0 0 48769 0 --:--:-- --:--:-- --:--:-- 66696 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 3824343 spots for /home/okishinya/chipatlas/results/dm3/SRX013036/SRR030302.sra Written 3824343 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:41 3824343 reads; of these: 3824343 (100.00%) were unpaired; of these: 1238888 (32.39%) aligned 0 times 2322330 (60.72%) aligned exactly 1 time 263125 (6.88%) aligned >1 times 67.61% overall alignment rate Time searching: 00:00:41 Overall time: 00:00:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 105995 / 2585455 = 0.0410 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 21 Apr 2015 12:00:22: # Command line: callpeak -t SRX013036.bam -f BAM -g dm -n SRX013036.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX013036.10 # format = BAM # ChIP-seq file = ['SRX013036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 12:00:22: # Command line: callpeak -t SRX013036.bam -f BAM -g dm -n SRX013036.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX013036.05 # format = BAM # ChIP-seq file = ['SRX013036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 12:00:22: #1 read tag files... INFO @ Tue, 21 Apr 2015 12:00:22: #1 read tag files... INFO @ Tue, 21 Apr 2015 12:00:22: # Command line: callpeak -t SRX013036.bam -f BAM -g dm -n SRX013036.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX013036.20 # format = BAM # ChIP-seq file = ['SRX013036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 12:00:22: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 12:00:22: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 12:00:22: #1 read tag files... INFO @ Tue, 21 Apr 2015 12:00:22: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 12:00:27: 1000000 INFO @ Tue, 21 Apr 2015 12:00:27: 1000000 INFO @ Tue, 21 Apr 2015 12:00:28: 1000000 INFO @ Tue, 21 Apr 2015 12:00:33: 2000000 INFO @ Tue, 21 Apr 2015 12:00:33: 2000000 INFO @ Tue, 21 Apr 2015 12:00:33: 2000000 INFO @ Tue, 21 Apr 2015 12:00:36: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 12:00:36: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 12:00:36: #1 total tags in treatment: 2479460 INFO @ Tue, 21 Apr 2015 12:00:36: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 12:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 12:00:36: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 12:00:36: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 12:00:36: #1 total tags in treatment: 2479460 INFO @ Tue, 21 Apr 2015 12:00:36: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 12:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 12:00:36: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 12:00:36: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 12:00:36: #1 total tags in treatment: 2479460 INFO @ Tue, 21 Apr 2015 12:00:36: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 12:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 12:00:36: #1 tags after filtering in treatment: 2479290 INFO @ Tue, 21 Apr 2015 12:00:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 12:00:36: #1 finished! INFO @ Tue, 21 Apr 2015 12:00:36: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 12:00:36: #1 tags after filtering in treatment: 2479290 INFO @ Tue, 21 Apr 2015 12:00:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 12:00:36: #1 finished! INFO @ Tue, 21 Apr 2015 12:00:36: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 12:00:36: #1 tags after filtering in treatment: 2479290 INFO @ Tue, 21 Apr 2015 12:00:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 12:00:36: #1 finished! INFO @ Tue, 21 Apr 2015 12:00:36: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 12:00:37: #2 number of paired peaks: 1441 INFO @ Tue, 21 Apr 2015 12:00:37: start model_add_line... INFO @ Tue, 21 Apr 2015 12:00:37: #2 number of paired peaks: 1441 INFO @ Tue, 21 Apr 2015 12:00:37: start model_add_line... INFO @ Tue, 21 Apr 2015 12:00:37: #2 number of paired peaks: 1441 INFO @ Tue, 21 Apr 2015 12:00:37: start model_add_line... INFO @ Tue, 21 Apr 2015 12:00:39: start X-correlation... INFO @ Tue, 21 Apr 2015 12:00:39: end of X-cor INFO @ Tue, 21 Apr 2015 12:00:39: #2 finished! INFO @ Tue, 21 Apr 2015 12:00:39: #2 predicted fragment length is 180 bps INFO @ Tue, 21 Apr 2015 12:00:39: #2 alternative fragment length(s) may be 180 bps INFO @ Tue, 21 Apr 2015 12:00:39: #2.2 Generate R script for model : SRX013036.05_model.r INFO @ Tue, 21 Apr 2015 12:00:39: #3 Call peaks... INFO @ Tue, 21 Apr 2015 12:00:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 12:00:39: start X-correlation... INFO @ Tue, 21 Apr 2015 12:00:39: end of X-cor INFO @ Tue, 21 Apr 2015 12:00:39: #2 finished! INFO @ Tue, 21 Apr 2015 12:00:39: #2 predicted fragment length is 180 bps INFO @ Tue, 21 Apr 2015 12:00:39: #2 alternative fragment length(s) may be 180 bps INFO @ Tue, 21 Apr 2015 12:00:39: #2.2 Generate R script for model : SRX013036.20_model.r INFO @ Tue, 21 Apr 2015 12:00:39: #3 Call peaks... INFO @ Tue, 21 Apr 2015 12:00:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 12:00:39: start X-correlation... INFO @ Tue, 21 Apr 2015 12:00:39: end of X-cor INFO @ Tue, 21 Apr 2015 12:00:39: #2 finished! INFO @ Tue, 21 Apr 2015 12:00:39: #2 predicted fragment length is 180 bps INFO @ Tue, 21 Apr 2015 12:00:39: #2 alternative fragment length(s) may be 180 bps INFO @ Tue, 21 Apr 2015 12:00:39: #2.2 Generate R script for model : SRX013036.10_model.r INFO @ Tue, 21 Apr 2015 12:00:39: #3 Call peaks... INFO @ Tue, 21 Apr 2015 12:00:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 12:00:52: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 12:00:53: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 12:00:54: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 12:01:03: #4 Write output xls file... SRX013036.20_peaks.xls INFO @ Tue, 21 Apr 2015 12:01:03: #4 Write peak in narrowPeak format file... SRX013036.20_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 12:01:03: #4 Write summits bed file... SRX013036.20_summits.bed INFO @ Tue, 21 Apr 2015 12:01:03: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (117 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 12:01:05: #4 Write output xls file... SRX013036.10_peaks.xls INFO @ Tue, 21 Apr 2015 12:01:05: #4 Write peak in narrowPeak format file... SRX013036.10_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 12:01:05: #4 Write summits bed file... SRX013036.10_summits.bed INFO @ Tue, 21 Apr 2015 12:01:05: Done! pass1 - making usageList (8 chroms): 0 millis pass2 - checking and writing primary data (850 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 12:01:06: #4 Write output xls file... SRX013036.05_peaks.xls INFO @ Tue, 21 Apr 2015 12:01:06: #4 Write peak in narrowPeak format file... SRX013036.05_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 12:01:06: #4 Write summits bed file... SRX013036.05_summits.bed INFO @ Tue, 21 Apr 2015 12:01:06: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (2527 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。