Job ID = 2161153 sra ファイルのダウンロード中... Completed: 60988K bytes transferred in 4 seconds (122135K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 34556 0 34556 0 0 49725 0 --:--:-- --:--:-- --:--:-- 68699 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 2262518 spots for /home/okishinya/chipatlas/results/dm3/SRX013033/SRR030299.sra Written 2262518 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:24 2262518 reads; of these: 2262518 (100.00%) were unpaired; of these: 970296 (42.89%) aligned 0 times 1151844 (50.91%) aligned exactly 1 time 140378 (6.20%) aligned >1 times 57.11% overall alignment rate Time searching: 00:00:24 Overall time: 00:00:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 57743 / 1292222 = 0.0447 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 21 Apr 2015 11:59:11: # Command line: callpeak -t SRX013033.bam -f BAM -g dm -n SRX013033.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX013033.10 # format = BAM # ChIP-seq file = ['SRX013033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:59:11: # Command line: callpeak -t SRX013033.bam -f BAM -g dm -n SRX013033.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX013033.05 # format = BAM # ChIP-seq file = ['SRX013033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:59:11: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:59:11: # Command line: callpeak -t SRX013033.bam -f BAM -g dm -n SRX013033.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX013033.20 # format = BAM # ChIP-seq file = ['SRX013033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:59:11: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:59:11: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:59:11: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:59:11: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:59:11: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:59:16: 1000000 INFO @ Tue, 21 Apr 2015 11:59:16: 1000000 INFO @ Tue, 21 Apr 2015 11:59:16: 1000000 INFO @ Tue, 21 Apr 2015 11:59:17: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:59:17: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:59:17: #1 total tags in treatment: 1234479 INFO @ Tue, 21 Apr 2015 11:59:17: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:59:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:59:17: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:59:17: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:59:17: #1 total tags in treatment: 1234479 INFO @ Tue, 21 Apr 2015 11:59:17: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:59:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:59:17: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:59:17: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:59:17: #1 total tags in treatment: 1234479 INFO @ Tue, 21 Apr 2015 11:59:17: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:59:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:59:17: #1 tags after filtering in treatment: 1234342 INFO @ Tue, 21 Apr 2015 11:59:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:59:17: #1 finished! INFO @ Tue, 21 Apr 2015 11:59:17: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:59:18: #1 tags after filtering in treatment: 1234342 INFO @ Tue, 21 Apr 2015 11:59:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:59:18: #1 finished! INFO @ Tue, 21 Apr 2015 11:59:18: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:59:18: #1 tags after filtering in treatment: 1234342 INFO @ Tue, 21 Apr 2015 11:59:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:59:18: #1 finished! INFO @ Tue, 21 Apr 2015 11:59:18: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:59:18: #2 number of paired peaks: 5394 INFO @ Tue, 21 Apr 2015 11:59:18: start model_add_line... INFO @ Tue, 21 Apr 2015 11:59:18: #2 number of paired peaks: 5394 INFO @ Tue, 21 Apr 2015 11:59:18: start model_add_line... INFO @ Tue, 21 Apr 2015 11:59:18: #2 number of paired peaks: 5394 INFO @ Tue, 21 Apr 2015 11:59:18: start model_add_line... INFO @ Tue, 21 Apr 2015 11:59:22: start X-correlation... INFO @ Tue, 21 Apr 2015 11:59:22: end of X-cor INFO @ Tue, 21 Apr 2015 11:59:22: #2 finished! INFO @ Tue, 21 Apr 2015 11:59:22: #2 predicted fragment length is 133 bps INFO @ Tue, 21 Apr 2015 11:59:22: #2 alternative fragment length(s) may be 133 bps INFO @ Tue, 21 Apr 2015 11:59:22: #2.2 Generate R script for model : SRX013033.20_model.r INFO @ Tue, 21 Apr 2015 11:59:22: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:59:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:59:22: start X-correlation... INFO @ Tue, 21 Apr 2015 11:59:22: end of X-cor INFO @ Tue, 21 Apr 2015 11:59:22: #2 finished! INFO @ Tue, 21 Apr 2015 11:59:22: #2 predicted fragment length is 133 bps INFO @ Tue, 21 Apr 2015 11:59:22: #2 alternative fragment length(s) may be 133 bps INFO @ Tue, 21 Apr 2015 11:59:22: #2.2 Generate R script for model : SRX013033.05_model.r INFO @ Tue, 21 Apr 2015 11:59:22: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:59:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:59:22: start X-correlation... INFO @ Tue, 21 Apr 2015 11:59:22: end of X-cor INFO @ Tue, 21 Apr 2015 11:59:22: #2 finished! INFO @ Tue, 21 Apr 2015 11:59:22: #2 predicted fragment length is 133 bps INFO @ Tue, 21 Apr 2015 11:59:22: #2 alternative fragment length(s) may be 133 bps INFO @ Tue, 21 Apr 2015 11:59:22: #2.2 Generate R script for model : SRX013033.10_model.r INFO @ Tue, 21 Apr 2015 11:59:22: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:59:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:59:29: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:59:29: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:59:30: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:59:34: #4 Write output xls file... SRX013033.20_peaks.xls INFO @ Tue, 21 Apr 2015 11:59:34: #4 Write peak in narrowPeak format file... SRX013033.20_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:59:34: #4 Write summits bed file... SRX013033.20_summits.bed INFO @ Tue, 21 Apr 2015 11:59:34: Done! pass1 - making usageList (2 chroms): 0 millis pass2 - checking and writing primary data (40 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 11:59:35: #4 Write output xls file... SRX013033.10_peaks.xls INFO @ Tue, 21 Apr 2015 11:59:35: #4 Write peak in narrowPeak format file... SRX013033.10_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:59:35: #4 Write summits bed file... SRX013033.10_summits.bed INFO @ Tue, 21 Apr 2015 11:59:35: Done! pass1 - making usageList (8 chroms): 0 millis pass2 - checking and writing primary data (393 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 11:59:36: #4 Write output xls file... SRX013033.05_peaks.xls INFO @ Tue, 21 Apr 2015 11:59:36: #4 Write peak in narrowPeak format file... SRX013033.05_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:59:36: #4 Write summits bed file... SRX013033.05_summits.bed INFO @ Tue, 21 Apr 2015 11:59:36: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (2700 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。