Job ID = 2161080 sra ファイルのダウンロード中... Completed: 72081K bytes transferred in 4 seconds (142758K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 14212 0 14212 0 0 20783 0 --:--:-- --:--:-- --:--:-- 28827 100 34685 0 34685 0 0 50659 0 --:--:-- --:--:-- --:--:-- 70212 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 2441451 spots for /home/okishinya/chipatlas/results/dm3/SRX013013/SRR030279.sra Written 2441451 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:30 2441451 reads; of these: 2441451 (100.00%) were unpaired; of these: 912886 (37.39%) aligned 0 times 1238952 (50.75%) aligned exactly 1 time 289613 (11.86%) aligned >1 times 62.61% overall alignment rate Time searching: 00:00:30 Overall time: 00:00:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 40463 / 1528565 = 0.0265 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 21 Apr 2015 11:54:51: # Command line: callpeak -t SRX013013.bam -f BAM -g dm -n SRX013013.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX013013.05 # format = BAM # ChIP-seq file = ['SRX013013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:54:51: # Command line: callpeak -t SRX013013.bam -f BAM -g dm -n SRX013013.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX013013.10 # format = BAM # ChIP-seq file = ['SRX013013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:54:51: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:54:51: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:54:51: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:54:51: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:54:51: # Command line: callpeak -t SRX013013.bam -f BAM -g dm -n SRX013013.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX013013.20 # format = BAM # ChIP-seq file = ['SRX013013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:54:51: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:54:51: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:54:57: 1000000 INFO @ Tue, 21 Apr 2015 11:54:57: 1000000 INFO @ Tue, 21 Apr 2015 11:54:57: 1000000 INFO @ Tue, 21 Apr 2015 11:54:59: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:54:59: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:54:59: #1 total tags in treatment: 1488102 INFO @ Tue, 21 Apr 2015 11:54:59: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:54:59: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:54:59: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:54:59: #1 total tags in treatment: 1488102 INFO @ Tue, 21 Apr 2015 11:54:59: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:54:59: #1 tags after filtering in treatment: 1488039 INFO @ Tue, 21 Apr 2015 11:54:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:54:59: #1 finished! INFO @ Tue, 21 Apr 2015 11:54:59: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:55:00: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:55:00: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:55:00: #1 total tags in treatment: 1488102 INFO @ Tue, 21 Apr 2015 11:55:00: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:55:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:55:00: #1 tags after filtering in treatment: 1488039 INFO @ Tue, 21 Apr 2015 11:55:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:55:00: #1 finished! INFO @ Tue, 21 Apr 2015 11:55:00: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:55:00: #2 number of paired peaks: 585 WARNING @ Tue, 21 Apr 2015 11:55:00: Fewer paired peaks (585) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 585 pairs to build model! INFO @ Tue, 21 Apr 2015 11:55:00: start model_add_line... INFO @ Tue, 21 Apr 2015 11:55:00: #1 tags after filtering in treatment: 1488039 INFO @ Tue, 21 Apr 2015 11:55:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:55:00: #1 finished! INFO @ Tue, 21 Apr 2015 11:55:00: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:55:00: #2 number of paired peaks: 585 WARNING @ Tue, 21 Apr 2015 11:55:00: Fewer paired peaks (585) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 585 pairs to build model! INFO @ Tue, 21 Apr 2015 11:55:00: start model_add_line... INFO @ Tue, 21 Apr 2015 11:55:00: #2 number of paired peaks: 585 WARNING @ Tue, 21 Apr 2015 11:55:00: Fewer paired peaks (585) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 585 pairs to build model! INFO @ Tue, 21 Apr 2015 11:55:00: start model_add_line... INFO @ Tue, 21 Apr 2015 11:55:00: start X-correlation... INFO @ Tue, 21 Apr 2015 11:55:00: end of X-cor INFO @ Tue, 21 Apr 2015 11:55:00: #2 finished! INFO @ Tue, 21 Apr 2015 11:55:00: #2 predicted fragment length is 118 bps INFO @ Tue, 21 Apr 2015 11:55:00: #2 alternative fragment length(s) may be 118 bps INFO @ Tue, 21 Apr 2015 11:55:00: #2.2 Generate R script for model : SRX013013.05_model.r INFO @ Tue, 21 Apr 2015 11:55:00: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:55:00: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:55:01: start X-correlation... INFO @ Tue, 21 Apr 2015 11:55:01: end of X-cor INFO @ Tue, 21 Apr 2015 11:55:01: #2 finished! INFO @ Tue, 21 Apr 2015 11:55:01: #2 predicted fragment length is 118 bps INFO @ Tue, 21 Apr 2015 11:55:01: #2 alternative fragment length(s) may be 118 bps INFO @ Tue, 21 Apr 2015 11:55:01: #2.2 Generate R script for model : SRX013013.20_model.r INFO @ Tue, 21 Apr 2015 11:55:01: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:55:01: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:55:01: start X-correlation... INFO @ Tue, 21 Apr 2015 11:55:01: end of X-cor INFO @ Tue, 21 Apr 2015 11:55:01: #2 finished! INFO @ Tue, 21 Apr 2015 11:55:01: #2 predicted fragment length is 118 bps INFO @ Tue, 21 Apr 2015 11:55:01: #2 alternative fragment length(s) may be 118 bps INFO @ Tue, 21 Apr 2015 11:55:01: #2.2 Generate R script for model : SRX013013.10_model.r INFO @ Tue, 21 Apr 2015 11:55:01: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:55:01: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:55:09: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:55:10: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:55:10: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:55:15: #4 Write output xls file... SRX013013.05_peaks.xls INFO @ Tue, 21 Apr 2015 11:55:15: #4 Write peak in narrowPeak format file... SRX013013.05_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:55:15: #4 Write summits bed file... SRX013013.05_summits.bed INFO @ Tue, 21 Apr 2015 11:55:15: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (288 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 11:55:16: #4 Write output xls file... SRX013013.20_peaks.xls INFO @ Tue, 21 Apr 2015 11:55:16: #4 Write peak in narrowPeak format file... SRX013013.20_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:55:16: #4 Write summits bed file... SRX013013.20_summits.bed INFO @ Tue, 21 Apr 2015 11:55:16: Done! pass1 - making usageList (1 chroms): 0 millis pass2 - checking and writing primary data (33 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 11:55:16: #4 Write output xls file... SRX013013.10_peaks.xls INFO @ Tue, 21 Apr 2015 11:55:16: #4 Write peak in narrowPeak format file... SRX013013.10_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:55:16: #4 Write summits bed file... SRX013013.10_summits.bed INFO @ Tue, 21 Apr 2015 11:55:16: Done! pass1 - making usageList (4 chroms): 0 millis pass2 - checking and writing primary data (90 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。