Job ID = 14168855 SRX = ERX3978894 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 63362904 spots for ERR3975963/ERR3975963.sra Written 63362904 spots for ERR3975963/ERR3975963.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170326 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 03:12:23 63362904 reads; of these: 63362904 (100.00%) were paired; of these: 7877440 (12.43%) aligned concordantly 0 times 41869073 (66.08%) aligned concordantly exactly 1 time 13616391 (21.49%) aligned concordantly >1 times ---- 7877440 pairs aligned concordantly 0 times; of these: 1042551 (13.23%) aligned discordantly 1 time ---- 6834889 pairs aligned 0 times concordantly or discordantly; of these: 13669778 mates make up the pairs; of these: 6092861 (44.57%) aligned 0 times 3837006 (28.07%) aligned exactly 1 time 3739911 (27.36%) aligned >1 times 95.19% overall alignment rate Time searching: 03:12:24 Overall time: 03:12:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 31963324 / 56187131 = 0.5689 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 00:47:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 00:47:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 00:47:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 00:47:54: 1000000 INFO @ Sat, 11 Dec 2021 00:47:59: 2000000 INFO @ Sat, 11 Dec 2021 00:48:04: 3000000 INFO @ Sat, 11 Dec 2021 00:48:10: 4000000 INFO @ Sat, 11 Dec 2021 00:48:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 00:48:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 00:48:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 00:48:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 00:48:21: 6000000 INFO @ Sat, 11 Dec 2021 00:48:25: 1000000 INFO @ Sat, 11 Dec 2021 00:48:27: 7000000 INFO @ Sat, 11 Dec 2021 00:48:31: 2000000 INFO @ Sat, 11 Dec 2021 00:48:33: 8000000 INFO @ Sat, 11 Dec 2021 00:48:37: 3000000 INFO @ Sat, 11 Dec 2021 00:48:39: 9000000 INFO @ Sat, 11 Dec 2021 00:48:43: 4000000 INFO @ Sat, 11 Dec 2021 00:48:45: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 00:48:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 00:48:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 00:48:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 00:48:49: 5000000 INFO @ Sat, 11 Dec 2021 00:48:52: 11000000 INFO @ Sat, 11 Dec 2021 00:48:55: 1000000 INFO @ Sat, 11 Dec 2021 00:48:56: 6000000 INFO @ Sat, 11 Dec 2021 00:48:58: 12000000 INFO @ Sat, 11 Dec 2021 00:49:02: 7000000 INFO @ Sat, 11 Dec 2021 00:49:02: 2000000 INFO @ Sat, 11 Dec 2021 00:49:05: 13000000 INFO @ Sat, 11 Dec 2021 00:49:09: 8000000 INFO @ Sat, 11 Dec 2021 00:49:10: 3000000 INFO @ Sat, 11 Dec 2021 00:49:12: 14000000 INFO @ Sat, 11 Dec 2021 00:49:16: 9000000 INFO @ Sat, 11 Dec 2021 00:49:17: 4000000 INFO @ Sat, 11 Dec 2021 00:49:18: 15000000 INFO @ Sat, 11 Dec 2021 00:49:22: 10000000 INFO @ Sat, 11 Dec 2021 00:49:24: 5000000 INFO @ Sat, 11 Dec 2021 00:49:25: 16000000 INFO @ Sat, 11 Dec 2021 00:49:29: 11000000 INFO @ Sat, 11 Dec 2021 00:49:31: 6000000 INFO @ Sat, 11 Dec 2021 00:49:32: 17000000 INFO @ Sat, 11 Dec 2021 00:49:35: 12000000 INFO @ Sat, 11 Dec 2021 00:49:38: 7000000 INFO @ Sat, 11 Dec 2021 00:49:38: 18000000 INFO @ Sat, 11 Dec 2021 00:49:42: 13000000 INFO @ Sat, 11 Dec 2021 00:49:45: 8000000 INFO @ Sat, 11 Dec 2021 00:49:45: 19000000 INFO @ Sat, 11 Dec 2021 00:49:49: 14000000 INFO @ Sat, 11 Dec 2021 00:49:52: 20000000 INFO @ Sat, 11 Dec 2021 00:49:52: 9000000 INFO @ Sat, 11 Dec 2021 00:49:55: 15000000 INFO @ Sat, 11 Dec 2021 00:49:58: 21000000 INFO @ Sat, 11 Dec 2021 00:49:59: 10000000 INFO @ Sat, 11 Dec 2021 00:50:02: 16000000 INFO @ Sat, 11 Dec 2021 00:50:05: 22000000 INFO @ Sat, 11 Dec 2021 00:50:06: 11000000 INFO @ Sat, 11 Dec 2021 00:50:08: 17000000 INFO @ Sat, 11 Dec 2021 00:50:12: 23000000 INFO @ Sat, 11 Dec 2021 00:50:13: 12000000 INFO @ Sat, 11 Dec 2021 00:50:15: 18000000 INFO @ Sat, 11 Dec 2021 00:50:19: 24000000 INFO @ Sat, 11 Dec 2021 00:50:20: 13000000 INFO @ Sat, 11 Dec 2021 00:50:21: 19000000 INFO @ Sat, 11 Dec 2021 00:50:25: 25000000 INFO @ Sat, 11 Dec 2021 00:50:27: 14000000 INFO @ Sat, 11 Dec 2021 00:50:28: 20000000 INFO @ Sat, 11 Dec 2021 00:50:32: 26000000 INFO @ Sat, 11 Dec 2021 00:50:34: 21000000 INFO @ Sat, 11 Dec 2021 00:50:34: 15000000 INFO @ Sat, 11 Dec 2021 00:50:39: 27000000 INFO @ Sat, 11 Dec 2021 00:50:41: 22000000 INFO @ Sat, 11 Dec 2021 00:50:41: 16000000 INFO @ Sat, 11 Dec 2021 00:50:45: 28000000 INFO @ Sat, 11 Dec 2021 00:50:47: 23000000 INFO @ Sat, 11 Dec 2021 00:50:48: 17000000 INFO @ Sat, 11 Dec 2021 00:50:52: 29000000 INFO @ Sat, 11 Dec 2021 00:50:54: 24000000 INFO @ Sat, 11 Dec 2021 00:50:55: 18000000 INFO @ Sat, 11 Dec 2021 00:50:58: 30000000 INFO @ Sat, 11 Dec 2021 00:51:00: 25000000 INFO @ Sat, 11 Dec 2021 00:51:02: 19000000 INFO @ Sat, 11 Dec 2021 00:51:05: 31000000 INFO @ Sat, 11 Dec 2021 00:51:07: 26000000 INFO @ Sat, 11 Dec 2021 00:51:09: 20000000 INFO @ Sat, 11 Dec 2021 00:51:11: 32000000 INFO @ Sat, 11 Dec 2021 00:51:13: 27000000 INFO @ Sat, 11 Dec 2021 00:51:16: 21000000 INFO @ Sat, 11 Dec 2021 00:51:18: 33000000 INFO @ Sat, 11 Dec 2021 00:51:20: 28000000 INFO @ Sat, 11 Dec 2021 00:51:23: 22000000 INFO @ Sat, 11 Dec 2021 00:51:25: 34000000 INFO @ Sat, 11 Dec 2021 00:51:26: 29000000 INFO @ Sat, 11 Dec 2021 00:51:30: 23000000 INFO @ Sat, 11 Dec 2021 00:51:31: 35000000 INFO @ Sat, 11 Dec 2021 00:51:33: 30000000 INFO @ Sat, 11 Dec 2021 00:51:37: 24000000 INFO @ Sat, 11 Dec 2021 00:51:38: 36000000 INFO @ Sat, 11 Dec 2021 00:51:39: 31000000 INFO @ Sat, 11 Dec 2021 00:51:44: 25000000 INFO @ Sat, 11 Dec 2021 00:51:45: 37000000 INFO @ Sat, 11 Dec 2021 00:51:46: 32000000 INFO @ Sat, 11 Dec 2021 00:51:51: 26000000 INFO @ Sat, 11 Dec 2021 00:51:52: 38000000 INFO @ Sat, 11 Dec 2021 00:51:53: 33000000 INFO @ Sat, 11 Dec 2021 00:51:58: 39000000 INFO @ Sat, 11 Dec 2021 00:51:58: 27000000 INFO @ Sat, 11 Dec 2021 00:51:59: 34000000 INFO @ Sat, 11 Dec 2021 00:52:05: 40000000 INFO @ Sat, 11 Dec 2021 00:52:05: 28000000 INFO @ Sat, 11 Dec 2021 00:52:06: 35000000 INFO @ Sat, 11 Dec 2021 00:52:12: 41000000 INFO @ Sat, 11 Dec 2021 00:52:12: 29000000 INFO @ Sat, 11 Dec 2021 00:52:13: 36000000 INFO @ Sat, 11 Dec 2021 00:52:18: 42000000 INFO @ Sat, 11 Dec 2021 00:52:19: 30000000 INFO @ Sat, 11 Dec 2021 00:52:19: 37000000 INFO @ Sat, 11 Dec 2021 00:52:25: 43000000 INFO @ Sat, 11 Dec 2021 00:52:26: 31000000 INFO @ Sat, 11 Dec 2021 00:52:26: 38000000 INFO @ Sat, 11 Dec 2021 00:52:32: 44000000 INFO @ Sat, 11 Dec 2021 00:52:33: 39000000 INFO @ Sat, 11 Dec 2021 00:52:33: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 00:52:39: 45000000 INFO @ Sat, 11 Dec 2021 00:52:39: 40000000 INFO @ Sat, 11 Dec 2021 00:52:40: 33000000 INFO @ Sat, 11 Dec 2021 00:52:45: 46000000 INFO @ Sat, 11 Dec 2021 00:52:46: 41000000 INFO @ Sat, 11 Dec 2021 00:52:47: 34000000 INFO @ Sat, 11 Dec 2021 00:52:52: 47000000 INFO @ Sat, 11 Dec 2021 00:52:53: 42000000 INFO @ Sat, 11 Dec 2021 00:52:54: 35000000 INFO @ Sat, 11 Dec 2021 00:52:59: 48000000 INFO @ Sat, 11 Dec 2021 00:53:00: 43000000 INFO @ Sat, 11 Dec 2021 00:53:01: 36000000 INFO @ Sat, 11 Dec 2021 00:53:06: 49000000 INFO @ Sat, 11 Dec 2021 00:53:06: 44000000 INFO @ Sat, 11 Dec 2021 00:53:08: 37000000 INFO @ Sat, 11 Dec 2021 00:53:12: 50000000 INFO @ Sat, 11 Dec 2021 00:53:13: 45000000 INFO @ Sat, 11 Dec 2021 00:53:15: 38000000 INFO @ Sat, 11 Dec 2021 00:53:19: 51000000 INFO @ Sat, 11 Dec 2021 00:53:20: 46000000 INFO @ Sat, 11 Dec 2021 00:53:22: 39000000 INFO @ Sat, 11 Dec 2021 00:53:25: 52000000 INFO @ Sat, 11 Dec 2021 00:53:27: 47000000 INFO @ Sat, 11 Dec 2021 00:53:29: 40000000 INFO @ Sat, 11 Dec 2021 00:53:32: 53000000 INFO @ Sat, 11 Dec 2021 00:53:33: 48000000 INFO @ Sat, 11 Dec 2021 00:53:36: 41000000 INFO @ Sat, 11 Dec 2021 00:53:38: 54000000 INFO @ Sat, 11 Dec 2021 00:53:40: 49000000 INFO @ Sat, 11 Dec 2021 00:53:43: 42000000 INFO @ Sat, 11 Dec 2021 00:53:45: 55000000 INFO @ Sat, 11 Dec 2021 00:53:46: 50000000 INFO @ Sat, 11 Dec 2021 00:53:50: 43000000 INFO @ Sat, 11 Dec 2021 00:53:51: 56000000 INFO @ Sat, 11 Dec 2021 00:53:53: 51000000 INFO @ Sat, 11 Dec 2021 00:53:56: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 00:53:56: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 00:53:56: #1 total tags in treatment: 24029927 INFO @ Sat, 11 Dec 2021 00:53:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 00:53:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 00:53:57: #1 tags after filtering in treatment: 21431102 INFO @ Sat, 11 Dec 2021 00:53:57: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 00:53:57: #1 finished! INFO @ Sat, 11 Dec 2021 00:53:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 00:53:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 00:53:58: 44000000 INFO @ Sat, 11 Dec 2021 00:53:58: #2 number of paired peaks: 126 WARNING @ Sat, 11 Dec 2021 00:53:58: Fewer paired peaks (126) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 126 pairs to build model! INFO @ Sat, 11 Dec 2021 00:53:58: start model_add_line... INFO @ Sat, 11 Dec 2021 00:53:58: start X-correlation... INFO @ Sat, 11 Dec 2021 00:53:58: end of X-cor INFO @ Sat, 11 Dec 2021 00:53:58: #2 finished! INFO @ Sat, 11 Dec 2021 00:53:58: #2 predicted fragment length is 129 bps INFO @ Sat, 11 Dec 2021 00:53:58: #2 alternative fragment length(s) may be 129 bps INFO @ Sat, 11 Dec 2021 00:53:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.05_model.r WARNING @ Sat, 11 Dec 2021 00:53:58: #2 Since the d (129) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 00:53:58: #2 You may need to consider one of the other alternative d(s): 129 WARNING @ Sat, 11 Dec 2021 00:53:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 00:53:58: #3 Call peaks... INFO @ Sat, 11 Dec 2021 00:53:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 00:54:00: 52000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 00:54:05: 45000000 INFO @ Sat, 11 Dec 2021 00:54:06: 53000000 INFO @ Sat, 11 Dec 2021 00:54:12: 46000000 INFO @ Sat, 11 Dec 2021 00:54:13: 54000000 INFO @ Sat, 11 Dec 2021 00:54:19: 47000000 INFO @ Sat, 11 Dec 2021 00:54:19: 55000000 INFO @ Sat, 11 Dec 2021 00:54:26: 56000000 INFO @ Sat, 11 Dec 2021 00:54:26: 48000000 INFO @ Sat, 11 Dec 2021 00:54:30: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 00:54:30: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 00:54:30: #1 total tags in treatment: 24029927 INFO @ Sat, 11 Dec 2021 00:54:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 00:54:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 00:54:31: #1 tags after filtering in treatment: 21431102 INFO @ Sat, 11 Dec 2021 00:54:31: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 00:54:31: #1 finished! INFO @ Sat, 11 Dec 2021 00:54:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 00:54:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 00:54:32: #2 number of paired peaks: 126 WARNING @ Sat, 11 Dec 2021 00:54:32: Fewer paired peaks (126) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 126 pairs to build model! INFO @ Sat, 11 Dec 2021 00:54:32: start model_add_line... INFO @ Sat, 11 Dec 2021 00:54:32: start X-correlation... INFO @ Sat, 11 Dec 2021 00:54:32: end of X-cor INFO @ Sat, 11 Dec 2021 00:54:32: #2 finished! INFO @ Sat, 11 Dec 2021 00:54:32: #2 predicted fragment length is 129 bps INFO @ Sat, 11 Dec 2021 00:54:32: #2 alternative fragment length(s) may be 129 bps INFO @ Sat, 11 Dec 2021 00:54:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.10_model.r WARNING @ Sat, 11 Dec 2021 00:54:32: #2 Since the d (129) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 00:54:32: #2 You may need to consider one of the other alternative d(s): 129 WARNING @ Sat, 11 Dec 2021 00:54:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 00:54:32: #3 Call peaks... INFO @ Sat, 11 Dec 2021 00:54:32: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 00:54:33: 49000000 INFO @ Sat, 11 Dec 2021 00:54:38: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 00:54:40: 50000000 INFO @ Sat, 11 Dec 2021 00:54:47: 51000000 INFO @ Sat, 11 Dec 2021 00:54:53: 52000000 INFO @ Sat, 11 Dec 2021 00:54:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.05_peaks.xls INFO @ Sat, 11 Dec 2021 00:54:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 00:54:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.05_summits.bed INFO @ Sat, 11 Dec 2021 00:54:59: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (12080 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 00:55:00: 53000000 INFO @ Sat, 11 Dec 2021 00:55:06: 54000000 INFO @ Sat, 11 Dec 2021 00:55:11: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 00:55:12: 55000000 INFO @ Sat, 11 Dec 2021 00:55:19: 56000000 INFO @ Sat, 11 Dec 2021 00:55:24: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 00:55:24: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 00:55:24: #1 total tags in treatment: 24029927 INFO @ Sat, 11 Dec 2021 00:55:24: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 00:55:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 00:55:24: #1 tags after filtering in treatment: 21431102 INFO @ Sat, 11 Dec 2021 00:55:24: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 00:55:24: #1 finished! INFO @ Sat, 11 Dec 2021 00:55:24: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 00:55:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 00:55:25: #2 number of paired peaks: 126 WARNING @ Sat, 11 Dec 2021 00:55:25: Fewer paired peaks (126) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 126 pairs to build model! INFO @ Sat, 11 Dec 2021 00:55:25: start model_add_line... INFO @ Sat, 11 Dec 2021 00:55:25: start X-correlation... INFO @ Sat, 11 Dec 2021 00:55:25: end of X-cor INFO @ Sat, 11 Dec 2021 00:55:25: #2 finished! INFO @ Sat, 11 Dec 2021 00:55:25: #2 predicted fragment length is 129 bps INFO @ Sat, 11 Dec 2021 00:55:25: #2 alternative fragment length(s) may be 129 bps INFO @ Sat, 11 Dec 2021 00:55:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.20_model.r WARNING @ Sat, 11 Dec 2021 00:55:25: #2 Since the d (129) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 00:55:25: #2 You may need to consider one of the other alternative d(s): 129 WARNING @ Sat, 11 Dec 2021 00:55:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 00:55:25: #3 Call peaks... INFO @ Sat, 11 Dec 2021 00:55:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 00:55:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.10_peaks.xls INFO @ Sat, 11 Dec 2021 00:55:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 00:55:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.10_summits.bed INFO @ Sat, 11 Dec 2021 00:55:31: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6323 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 00:56:05: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 00:56:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.20_peaks.xls INFO @ Sat, 11 Dec 2021 00:56:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 00:56:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978894/ERX3978894.20_summits.bed INFO @ Sat, 11 Dec 2021 00:56:26: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (2049 records, 4 fields): 5 millis CompletedMACS2peakCalling