Job ID = 14168754 SRX = ERX3978887 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 45829452 spots for ERR3975956/ERR3975956.sra Written 45829452 spots for ERR3975956/ERR3975956.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169964 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:08:40 45829452 reads; of these: 45829452 (100.00%) were paired; of these: 5480832 (11.96%) aligned concordantly 0 times 30626431 (66.83%) aligned concordantly exactly 1 time 9722189 (21.21%) aligned concordantly >1 times ---- 5480832 pairs aligned concordantly 0 times; of these: 883727 (16.12%) aligned discordantly 1 time ---- 4597105 pairs aligned 0 times concordantly or discordantly; of these: 9194210 mates make up the pairs; of these: 4624106 (50.29%) aligned 0 times 2154232 (23.43%) aligned exactly 1 time 2415872 (26.28%) aligned >1 times 94.96% overall alignment rate Time searching: 02:08:40 Overall time: 02:08:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 24278297 / 41035328 = 0.5916 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:29:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:29:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:29:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:29:23: 1000000 INFO @ Fri, 10 Dec 2021 22:29:29: 2000000 INFO @ Fri, 10 Dec 2021 22:29:35: 3000000 INFO @ Fri, 10 Dec 2021 22:29:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:29:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:29:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:29:47: 5000000 INFO @ Fri, 10 Dec 2021 22:29:55: 6000000 INFO @ Fri, 10 Dec 2021 22:29:56: 1000000 INFO @ Fri, 10 Dec 2021 22:30:03: 7000000 INFO @ Fri, 10 Dec 2021 22:30:05: 2000000 INFO @ Fri, 10 Dec 2021 22:30:12: 8000000 INFO @ Fri, 10 Dec 2021 22:30:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:30:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:30:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:30:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:30:20: 9000000 INFO @ Fri, 10 Dec 2021 22:30:23: 4000000 INFO @ Fri, 10 Dec 2021 22:30:26: 1000000 INFO @ Fri, 10 Dec 2021 22:30:28: 10000000 INFO @ Fri, 10 Dec 2021 22:30:33: 5000000 INFO @ Fri, 10 Dec 2021 22:30:34: 2000000 INFO @ Fri, 10 Dec 2021 22:30:36: 11000000 INFO @ Fri, 10 Dec 2021 22:30:42: 6000000 INFO @ Fri, 10 Dec 2021 22:30:43: 3000000 INFO @ Fri, 10 Dec 2021 22:30:44: 12000000 INFO @ Fri, 10 Dec 2021 22:30:51: 7000000 INFO @ Fri, 10 Dec 2021 22:30:52: 4000000 INFO @ Fri, 10 Dec 2021 22:30:52: 13000000 INFO @ Fri, 10 Dec 2021 22:30:59: 8000000 INFO @ Fri, 10 Dec 2021 22:31:01: 14000000 INFO @ Fri, 10 Dec 2021 22:31:01: 5000000 INFO @ Fri, 10 Dec 2021 22:31:09: 9000000 INFO @ Fri, 10 Dec 2021 22:31:09: 15000000 INFO @ Fri, 10 Dec 2021 22:31:10: 6000000 INFO @ Fri, 10 Dec 2021 22:31:17: 16000000 INFO @ Fri, 10 Dec 2021 22:31:18: 10000000 INFO @ Fri, 10 Dec 2021 22:31:19: 7000000 INFO @ Fri, 10 Dec 2021 22:31:25: 17000000 INFO @ Fri, 10 Dec 2021 22:31:27: 11000000 INFO @ Fri, 10 Dec 2021 22:31:28: 8000000 INFO @ Fri, 10 Dec 2021 22:31:33: 18000000 INFO @ Fri, 10 Dec 2021 22:31:36: 12000000 INFO @ Fri, 10 Dec 2021 22:31:37: 9000000 INFO @ Fri, 10 Dec 2021 22:31:41: 19000000 INFO @ Fri, 10 Dec 2021 22:31:45: 13000000 INFO @ Fri, 10 Dec 2021 22:31:45: 10000000 INFO @ Fri, 10 Dec 2021 22:31:49: 20000000 INFO @ Fri, 10 Dec 2021 22:31:54: 14000000 INFO @ Fri, 10 Dec 2021 22:31:54: 11000000 INFO @ Fri, 10 Dec 2021 22:31:57: 21000000 INFO @ Fri, 10 Dec 2021 22:32:03: 12000000 INFO @ Fri, 10 Dec 2021 22:32:03: 15000000 INFO @ Fri, 10 Dec 2021 22:32:05: 22000000 INFO @ Fri, 10 Dec 2021 22:32:12: 13000000 INFO @ Fri, 10 Dec 2021 22:32:12: 16000000 INFO @ Fri, 10 Dec 2021 22:32:13: 23000000 INFO @ Fri, 10 Dec 2021 22:32:21: 17000000 INFO @ Fri, 10 Dec 2021 22:32:21: 14000000 INFO @ Fri, 10 Dec 2021 22:32:21: 24000000 INFO @ Fri, 10 Dec 2021 22:32:30: 25000000 INFO @ Fri, 10 Dec 2021 22:32:30: 18000000 INFO @ Fri, 10 Dec 2021 22:32:30: 15000000 INFO @ Fri, 10 Dec 2021 22:32:38: 26000000 INFO @ Fri, 10 Dec 2021 22:32:39: 16000000 INFO @ Fri, 10 Dec 2021 22:32:39: 19000000 INFO @ Fri, 10 Dec 2021 22:32:46: 27000000 INFO @ Fri, 10 Dec 2021 22:32:48: 17000000 INFO @ Fri, 10 Dec 2021 22:32:48: 20000000 INFO @ Fri, 10 Dec 2021 22:32:54: 28000000 INFO @ Fri, 10 Dec 2021 22:32:57: 18000000 INFO @ Fri, 10 Dec 2021 22:32:57: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 22:33:02: 29000000 INFO @ Fri, 10 Dec 2021 22:33:05: 19000000 INFO @ Fri, 10 Dec 2021 22:33:06: 22000000 INFO @ Fri, 10 Dec 2021 22:33:11: 30000000 INFO @ Fri, 10 Dec 2021 22:33:14: 20000000 INFO @ Fri, 10 Dec 2021 22:33:16: 23000000 INFO @ Fri, 10 Dec 2021 22:33:19: 31000000 INFO @ Fri, 10 Dec 2021 22:33:23: 21000000 INFO @ Fri, 10 Dec 2021 22:33:25: 24000000 INFO @ Fri, 10 Dec 2021 22:33:27: 32000000 INFO @ Fri, 10 Dec 2021 22:33:31: 22000000 INFO @ Fri, 10 Dec 2021 22:33:34: 25000000 INFO @ Fri, 10 Dec 2021 22:33:36: 33000000 INFO @ Fri, 10 Dec 2021 22:33:40: 23000000 INFO @ Fri, 10 Dec 2021 22:33:44: 26000000 INFO @ Fri, 10 Dec 2021 22:33:44: 34000000 INFO @ Fri, 10 Dec 2021 22:33:49: 24000000 INFO @ Fri, 10 Dec 2021 22:33:52: 35000000 INFO @ Fri, 10 Dec 2021 22:33:53: 27000000 INFO @ Fri, 10 Dec 2021 22:33:58: 25000000 INFO @ Fri, 10 Dec 2021 22:34:01: 36000000 INFO @ Fri, 10 Dec 2021 22:34:02: 28000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 22:34:07: 26000000 INFO @ Fri, 10 Dec 2021 22:34:09: 37000000 INFO @ Fri, 10 Dec 2021 22:34:11: 29000000 INFO @ Fri, 10 Dec 2021 22:34:16: 27000000 INFO @ Fri, 10 Dec 2021 22:34:17: 38000000 INFO @ Fri, 10 Dec 2021 22:34:20: 30000000 INFO @ Fri, 10 Dec 2021 22:34:21: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 22:34:21: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 22:34:21: #1 total tags in treatment: 16493001 INFO @ Fri, 10 Dec 2021 22:34:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:34:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:34:21: #1 tags after filtering in treatment: 14867081 INFO @ Fri, 10 Dec 2021 22:34:21: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 22:34:21: #1 finished! INFO @ Fri, 10 Dec 2021 22:34:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:34:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:34:22: #2 number of paired peaks: 456 WARNING @ Fri, 10 Dec 2021 22:34:22: Fewer paired peaks (456) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 456 pairs to build model! INFO @ Fri, 10 Dec 2021 22:34:22: start model_add_line... INFO @ Fri, 10 Dec 2021 22:34:22: start X-correlation... INFO @ Fri, 10 Dec 2021 22:34:22: end of X-cor INFO @ Fri, 10 Dec 2021 22:34:22: #2 finished! INFO @ Fri, 10 Dec 2021 22:34:22: #2 predicted fragment length is 160 bps INFO @ Fri, 10 Dec 2021 22:34:22: #2 alternative fragment length(s) may be 160 bps INFO @ Fri, 10 Dec 2021 22:34:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.05_model.r WARNING @ Fri, 10 Dec 2021 22:34:22: #2 Since the d (160) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:34:22: #2 You may need to consider one of the other alternative d(s): 160 WARNING @ Fri, 10 Dec 2021 22:34:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:34:22: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:34:22: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:34:25: 28000000 INFO @ Fri, 10 Dec 2021 22:34:29: 31000000 INFO @ Fri, 10 Dec 2021 22:34:34: 29000000 INFO @ Fri, 10 Dec 2021 22:34:39: 32000000 INFO @ Fri, 10 Dec 2021 22:34:43: 30000000 INFO @ Fri, 10 Dec 2021 22:34:48: 33000000 INFO @ Fri, 10 Dec 2021 22:34:52: 31000000 INFO @ Fri, 10 Dec 2021 22:34:52: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:34:57: 34000000 INFO @ Fri, 10 Dec 2021 22:35:01: 32000000 INFO @ Fri, 10 Dec 2021 22:35:07: 35000000 INFO @ Fri, 10 Dec 2021 22:35:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.05_peaks.xls INFO @ Fri, 10 Dec 2021 22:35:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:35:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.05_summits.bed INFO @ Fri, 10 Dec 2021 22:35:07: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (9747 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:35:10: 33000000 INFO @ Fri, 10 Dec 2021 22:35:16: 36000000 INFO @ Fri, 10 Dec 2021 22:35:19: 34000000 INFO @ Fri, 10 Dec 2021 22:35:25: 37000000 INFO @ Fri, 10 Dec 2021 22:35:29: 35000000 INFO @ Fri, 10 Dec 2021 22:35:34: 38000000 INFO @ Fri, 10 Dec 2021 22:35:37: 36000000 INFO @ Fri, 10 Dec 2021 22:35:38: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 22:35:38: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 22:35:38: #1 total tags in treatment: 16493001 INFO @ Fri, 10 Dec 2021 22:35:38: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:35:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:35:38: #1 tags after filtering in treatment: 14867081 INFO @ Fri, 10 Dec 2021 22:35:38: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 22:35:38: #1 finished! INFO @ Fri, 10 Dec 2021 22:35:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:35:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:35:39: #2 number of paired peaks: 456 WARNING @ Fri, 10 Dec 2021 22:35:39: Fewer paired peaks (456) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 456 pairs to build model! INFO @ Fri, 10 Dec 2021 22:35:39: start model_add_line... INFO @ Fri, 10 Dec 2021 22:35:39: start X-correlation... INFO @ Fri, 10 Dec 2021 22:35:39: end of X-cor INFO @ Fri, 10 Dec 2021 22:35:39: #2 finished! INFO @ Fri, 10 Dec 2021 22:35:39: #2 predicted fragment length is 160 bps INFO @ Fri, 10 Dec 2021 22:35:39: #2 alternative fragment length(s) may be 160 bps INFO @ Fri, 10 Dec 2021 22:35:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.10_model.r WARNING @ Fri, 10 Dec 2021 22:35:39: #2 Since the d (160) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:35:39: #2 You may need to consider one of the other alternative d(s): 160 WARNING @ Fri, 10 Dec 2021 22:35:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:35:39: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:35:39: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:35:45: 37000000 INFO @ Fri, 10 Dec 2021 22:35:53: 38000000 INFO @ Fri, 10 Dec 2021 22:35:57: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 22:35:57: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 22:35:57: #1 total tags in treatment: 16493001 INFO @ Fri, 10 Dec 2021 22:35:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:35:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:35:57: #1 tags after filtering in treatment: 14867081 INFO @ Fri, 10 Dec 2021 22:35:57: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 22:35:57: #1 finished! INFO @ Fri, 10 Dec 2021 22:35:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:35:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:35:58: #2 number of paired peaks: 456 WARNING @ Fri, 10 Dec 2021 22:35:58: Fewer paired peaks (456) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 456 pairs to build model! INFO @ Fri, 10 Dec 2021 22:35:58: start model_add_line... INFO @ Fri, 10 Dec 2021 22:35:59: start X-correlation... INFO @ Fri, 10 Dec 2021 22:35:59: end of X-cor INFO @ Fri, 10 Dec 2021 22:35:59: #2 finished! INFO @ Fri, 10 Dec 2021 22:35:59: #2 predicted fragment length is 160 bps INFO @ Fri, 10 Dec 2021 22:35:59: #2 alternative fragment length(s) may be 160 bps INFO @ Fri, 10 Dec 2021 22:35:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.20_model.r WARNING @ Fri, 10 Dec 2021 22:35:59: #2 Since the d (160) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:35:59: #2 You may need to consider one of the other alternative d(s): 160 WARNING @ Fri, 10 Dec 2021 22:35:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:35:59: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:35:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:36:08: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:36:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.10_peaks.xls INFO @ Fri, 10 Dec 2021 22:36:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:36:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.10_summits.bed INFO @ Fri, 10 Dec 2021 22:36:23: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (5001 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:36:27: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:36:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.20_peaks.xls INFO @ Fri, 10 Dec 2021 22:36:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:36:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978887/ERX3978887.20_summits.bed INFO @ Fri, 10 Dec 2021 22:36:42: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (1997 records, 4 fields): 4 millis CompletedMACS2peakCalling