Job ID = 14168719 SRX = ERX3978881 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 38367909 spots for ERR3975950/ERR3975950.sra Written 38367909 spots for ERR3975950/ERR3975950.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170082 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 03:33:24 38367909 reads; of these: 38367909 (100.00%) were paired; of these: 7233357 (18.85%) aligned concordantly 0 times 17366617 (45.26%) aligned concordantly exactly 1 time 13767935 (35.88%) aligned concordantly >1 times ---- 7233357 pairs aligned concordantly 0 times; of these: 623041 (8.61%) aligned discordantly 1 time ---- 6610316 pairs aligned 0 times concordantly or discordantly; of these: 13220632 mates make up the pairs; of these: 5668870 (42.88%) aligned 0 times 2333293 (17.65%) aligned exactly 1 time 5218469 (39.47%) aligned >1 times 92.61% overall alignment rate Time searching: 03:33:25 Overall time: 03:33:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10359247 / 31410110 = 0.3298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 23:49:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 23:49:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 23:49:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 23:49:31: 1000000 INFO @ Fri, 10 Dec 2021 23:49:37: 2000000 INFO @ Fri, 10 Dec 2021 23:49:43: 3000000 INFO @ Fri, 10 Dec 2021 23:49:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 23:49:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 23:49:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 23:49:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 23:49:55: 5000000 INFO @ Fri, 10 Dec 2021 23:50:02: 1000000 INFO @ Fri, 10 Dec 2021 23:50:03: 6000000 INFO @ Fri, 10 Dec 2021 23:50:10: 7000000 INFO @ Fri, 10 Dec 2021 23:50:11: 2000000 INFO @ Fri, 10 Dec 2021 23:50:17: 8000000 INFO @ Fri, 10 Dec 2021 23:50:19: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 23:50:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 23:50:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 23:50:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 23:50:25: 9000000 INFO @ Fri, 10 Dec 2021 23:50:27: 4000000 INFO @ Fri, 10 Dec 2021 23:50:32: 1000000 INFO @ Fri, 10 Dec 2021 23:50:32: 10000000 INFO @ Fri, 10 Dec 2021 23:50:36: 5000000 INFO @ Fri, 10 Dec 2021 23:50:40: 2000000 INFO @ Fri, 10 Dec 2021 23:50:40: 11000000 INFO @ Fri, 10 Dec 2021 23:50:44: 6000000 INFO @ Fri, 10 Dec 2021 23:50:47: 3000000 INFO @ Fri, 10 Dec 2021 23:50:48: 12000000 INFO @ Fri, 10 Dec 2021 23:50:53: 7000000 INFO @ Fri, 10 Dec 2021 23:50:55: 4000000 INFO @ Fri, 10 Dec 2021 23:50:56: 13000000 INFO @ Fri, 10 Dec 2021 23:51:01: 8000000 INFO @ Fri, 10 Dec 2021 23:51:03: 5000000 INFO @ Fri, 10 Dec 2021 23:51:04: 14000000 INFO @ Fri, 10 Dec 2021 23:51:09: 9000000 INFO @ Fri, 10 Dec 2021 23:51:11: 6000000 INFO @ Fri, 10 Dec 2021 23:51:11: 15000000 INFO @ Fri, 10 Dec 2021 23:51:17: 10000000 INFO @ Fri, 10 Dec 2021 23:51:18: 7000000 INFO @ Fri, 10 Dec 2021 23:51:19: 16000000 INFO @ Fri, 10 Dec 2021 23:51:26: 11000000 INFO @ Fri, 10 Dec 2021 23:51:26: 8000000 INFO @ Fri, 10 Dec 2021 23:51:27: 17000000 INFO @ Fri, 10 Dec 2021 23:51:33: 9000000 INFO @ Fri, 10 Dec 2021 23:51:34: 12000000 INFO @ Fri, 10 Dec 2021 23:51:34: 18000000 INFO @ Fri, 10 Dec 2021 23:51:41: 10000000 INFO @ Fri, 10 Dec 2021 23:51:42: 19000000 INFO @ Fri, 10 Dec 2021 23:51:42: 13000000 INFO @ Fri, 10 Dec 2021 23:51:49: 11000000 INFO @ Fri, 10 Dec 2021 23:51:49: 20000000 INFO @ Fri, 10 Dec 2021 23:51:50: 14000000 INFO @ Fri, 10 Dec 2021 23:51:56: 12000000 INFO @ Fri, 10 Dec 2021 23:51:57: 21000000 INFO @ Fri, 10 Dec 2021 23:51:59: 15000000 INFO @ Fri, 10 Dec 2021 23:52:04: 13000000 INFO @ Fri, 10 Dec 2021 23:52:04: 22000000 INFO @ Fri, 10 Dec 2021 23:52:07: 16000000 INFO @ Fri, 10 Dec 2021 23:52:12: 14000000 INFO @ Fri, 10 Dec 2021 23:52:12: 23000000 INFO @ Fri, 10 Dec 2021 23:52:15: 17000000 INFO @ Fri, 10 Dec 2021 23:52:19: 15000000 INFO @ Fri, 10 Dec 2021 23:52:19: 24000000 INFO @ Fri, 10 Dec 2021 23:52:23: 18000000 INFO @ Fri, 10 Dec 2021 23:52:27: 16000000 INFO @ Fri, 10 Dec 2021 23:52:27: 25000000 INFO @ Fri, 10 Dec 2021 23:52:31: 19000000 INFO @ Fri, 10 Dec 2021 23:52:34: 17000000 INFO @ Fri, 10 Dec 2021 23:52:34: 26000000 INFO @ Fri, 10 Dec 2021 23:52:39: 20000000 INFO @ Fri, 10 Dec 2021 23:52:42: 18000000 INFO @ Fri, 10 Dec 2021 23:52:42: 27000000 INFO @ Fri, 10 Dec 2021 23:52:47: 21000000 INFO @ Fri, 10 Dec 2021 23:52:49: 19000000 INFO @ Fri, 10 Dec 2021 23:52:50: 28000000 INFO @ Fri, 10 Dec 2021 23:52:55: 22000000 INFO @ Fri, 10 Dec 2021 23:52:57: 20000000 INFO @ Fri, 10 Dec 2021 23:52:57: 29000000 INFO @ Fri, 10 Dec 2021 23:53:03: 23000000 INFO @ Fri, 10 Dec 2021 23:53:04: 21000000 INFO @ Fri, 10 Dec 2021 23:53:05: 30000000 INFO @ Fri, 10 Dec 2021 23:53:11: 24000000 INFO @ Fri, 10 Dec 2021 23:53:12: 22000000 INFO @ Fri, 10 Dec 2021 23:53:12: 31000000 INFO @ Fri, 10 Dec 2021 23:53:19: 25000000 INFO @ Fri, 10 Dec 2021 23:53:19: 23000000 INFO @ Fri, 10 Dec 2021 23:53:20: 32000000 INFO @ Fri, 10 Dec 2021 23:53:26: 24000000 INFO @ Fri, 10 Dec 2021 23:53:27: 26000000 INFO @ Fri, 10 Dec 2021 23:53:28: 33000000 INFO @ Fri, 10 Dec 2021 23:53:34: 25000000 INFO @ Fri, 10 Dec 2021 23:53:35: 34000000 INFO @ Fri, 10 Dec 2021 23:53:36: 27000000 INFO @ Fri, 10 Dec 2021 23:53:41: 26000000 INFO @ Fri, 10 Dec 2021 23:53:43: 35000000 INFO @ Fri, 10 Dec 2021 23:53:44: 28000000 INFO @ Fri, 10 Dec 2021 23:53:49: 27000000 INFO @ Fri, 10 Dec 2021 23:53:50: 36000000 INFO @ Fri, 10 Dec 2021 23:53:53: 29000000 INFO @ Fri, 10 Dec 2021 23:53:56: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 23:53:58: 37000000 INFO @ Fri, 10 Dec 2021 23:54:02: 30000000 INFO @ Fri, 10 Dec 2021 23:54:04: 29000000 INFO @ Fri, 10 Dec 2021 23:54:05: 38000000 INFO @ Fri, 10 Dec 2021 23:54:10: 31000000 INFO @ Fri, 10 Dec 2021 23:54:12: 30000000 INFO @ Fri, 10 Dec 2021 23:54:13: 39000000 INFO @ Fri, 10 Dec 2021 23:54:18: 32000000 INFO @ Fri, 10 Dec 2021 23:54:19: 31000000 INFO @ Fri, 10 Dec 2021 23:54:20: 40000000 INFO @ Fri, 10 Dec 2021 23:54:26: 32000000 INFO @ Fri, 10 Dec 2021 23:54:27: 33000000 INFO @ Fri, 10 Dec 2021 23:54:28: 41000000 INFO @ Fri, 10 Dec 2021 23:54:34: 33000000 INFO @ Fri, 10 Dec 2021 23:54:35: 42000000 INFO @ Fri, 10 Dec 2021 23:54:35: 34000000 INFO @ Fri, 10 Dec 2021 23:54:41: 34000000 INFO @ Fri, 10 Dec 2021 23:54:43: 43000000 INFO @ Fri, 10 Dec 2021 23:54:44: 35000000 INFO @ Fri, 10 Dec 2021 23:54:49: 35000000 INFO @ Fri, 10 Dec 2021 23:54:50: 44000000 INFO @ Fri, 10 Dec 2021 23:54:52: 36000000 INFO @ Fri, 10 Dec 2021 23:54:56: 36000000 INFO @ Fri, 10 Dec 2021 23:54:57: 45000000 INFO @ Fri, 10 Dec 2021 23:55:00: 37000000 INFO @ Fri, 10 Dec 2021 23:55:04: 37000000 INFO @ Fri, 10 Dec 2021 23:55:05: 46000000 INFO @ Fri, 10 Dec 2021 23:55:09: 38000000 INFO @ Fri, 10 Dec 2021 23:55:11: 38000000 INFO @ Fri, 10 Dec 2021 23:55:13: 47000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 23:55:17: 39000000 INFO @ Fri, 10 Dec 2021 23:55:19: 39000000 INFO @ Fri, 10 Dec 2021 23:55:20: 48000000 INFO @ Fri, 10 Dec 2021 23:55:25: 40000000 INFO @ Fri, 10 Dec 2021 23:55:27: 40000000 INFO @ Fri, 10 Dec 2021 23:55:28: 49000000 INFO @ Fri, 10 Dec 2021 23:55:34: 41000000 INFO @ Fri, 10 Dec 2021 23:55:35: 41000000 INFO @ Fri, 10 Dec 2021 23:55:35: 50000000 INFO @ Fri, 10 Dec 2021 23:55:38: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 23:55:38: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 23:55:38: #1 total tags in treatment: 20855977 INFO @ Fri, 10 Dec 2021 23:55:38: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 23:55:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 23:55:38: #1 tags after filtering in treatment: 17906431 INFO @ Fri, 10 Dec 2021 23:55:38: #1 Redundant rate of treatment: 0.14 INFO @ Fri, 10 Dec 2021 23:55:38: #1 finished! INFO @ Fri, 10 Dec 2021 23:55:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 23:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 23:55:40: #2 number of paired peaks: 249 WARNING @ Fri, 10 Dec 2021 23:55:40: Fewer paired peaks (249) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 249 pairs to build model! INFO @ Fri, 10 Dec 2021 23:55:40: start model_add_line... INFO @ Fri, 10 Dec 2021 23:55:40: start X-correlation... INFO @ Fri, 10 Dec 2021 23:55:40: end of X-cor INFO @ Fri, 10 Dec 2021 23:55:40: #2 finished! INFO @ Fri, 10 Dec 2021 23:55:40: #2 predicted fragment length is 116 bps INFO @ Fri, 10 Dec 2021 23:55:40: #2 alternative fragment length(s) may be 116 bps INFO @ Fri, 10 Dec 2021 23:55:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.05_model.r WARNING @ Fri, 10 Dec 2021 23:55:40: #2 Since the d (116) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 23:55:40: #2 You may need to consider one of the other alternative d(s): 116 WARNING @ Fri, 10 Dec 2021 23:55:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 23:55:40: #3 Call peaks... INFO @ Fri, 10 Dec 2021 23:55:40: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 23:55:42: 42000000 INFO @ Fri, 10 Dec 2021 23:55:43: 42000000 INFO @ Fri, 10 Dec 2021 23:55:51: 43000000 INFO @ Fri, 10 Dec 2021 23:55:51: 43000000 INFO @ Fri, 10 Dec 2021 23:55:59: 44000000 INFO @ Fri, 10 Dec 2021 23:55:59: 44000000 INFO @ Fri, 10 Dec 2021 23:56:07: 45000000 INFO @ Fri, 10 Dec 2021 23:56:08: 45000000 INFO @ Fri, 10 Dec 2021 23:56:12: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 23:56:16: 46000000 INFO @ Fri, 10 Dec 2021 23:56:16: 46000000 INFO @ Fri, 10 Dec 2021 23:56:24: 47000000 INFO @ Fri, 10 Dec 2021 23:56:25: 47000000 INFO @ Fri, 10 Dec 2021 23:56:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.05_peaks.xls INFO @ Fri, 10 Dec 2021 23:56:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 23:56:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.05_summits.bed INFO @ Fri, 10 Dec 2021 23:56:28: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (8133 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 23:56:32: 48000000 INFO @ Fri, 10 Dec 2021 23:56:33: 48000000 INFO @ Fri, 10 Dec 2021 23:56:41: 49000000 INFO @ Fri, 10 Dec 2021 23:56:41: 49000000 INFO @ Fri, 10 Dec 2021 23:56:49: 50000000 INFO @ Fri, 10 Dec 2021 23:56:49: 50000000 INFO @ Fri, 10 Dec 2021 23:56:52: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 23:56:52: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 23:56:52: #1 total tags in treatment: 20855977 INFO @ Fri, 10 Dec 2021 23:56:52: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 23:56:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 23:56:52: #1 tags after filtering in treatment: 17906431 INFO @ Fri, 10 Dec 2021 23:56:52: #1 Redundant rate of treatment: 0.14 INFO @ Fri, 10 Dec 2021 23:56:52: #1 finished! INFO @ Fri, 10 Dec 2021 23:56:52: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 23:56:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 23:56:52: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 23:56:52: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 23:56:52: #1 total tags in treatment: 20855977 INFO @ Fri, 10 Dec 2021 23:56:52: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 23:56:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 23:56:53: #1 tags after filtering in treatment: 17906431 INFO @ Fri, 10 Dec 2021 23:56:53: #1 Redundant rate of treatment: 0.14 INFO @ Fri, 10 Dec 2021 23:56:53: #1 finished! INFO @ Fri, 10 Dec 2021 23:56:53: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 23:56:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 23:56:53: #2 number of paired peaks: 249 WARNING @ Fri, 10 Dec 2021 23:56:53: Fewer paired peaks (249) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 249 pairs to build model! INFO @ Fri, 10 Dec 2021 23:56:53: start model_add_line... INFO @ Fri, 10 Dec 2021 23:56:53: start X-correlation... INFO @ Fri, 10 Dec 2021 23:56:53: end of X-cor INFO @ Fri, 10 Dec 2021 23:56:53: #2 finished! INFO @ Fri, 10 Dec 2021 23:56:53: #2 predicted fragment length is 116 bps INFO @ Fri, 10 Dec 2021 23:56:53: #2 alternative fragment length(s) may be 116 bps INFO @ Fri, 10 Dec 2021 23:56:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.10_model.r WARNING @ Fri, 10 Dec 2021 23:56:53: #2 Since the d (116) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 23:56:53: #2 You may need to consider one of the other alternative d(s): 116 WARNING @ Fri, 10 Dec 2021 23:56:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 23:56:53: #3 Call peaks... INFO @ Fri, 10 Dec 2021 23:56:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 23:56:54: #2 number of paired peaks: 249 WARNING @ Fri, 10 Dec 2021 23:56:54: Fewer paired peaks (249) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 249 pairs to build model! INFO @ Fri, 10 Dec 2021 23:56:54: start model_add_line... INFO @ Fri, 10 Dec 2021 23:56:54: start X-correlation... INFO @ Fri, 10 Dec 2021 23:56:54: end of X-cor INFO @ Fri, 10 Dec 2021 23:56:54: #2 finished! INFO @ Fri, 10 Dec 2021 23:56:54: #2 predicted fragment length is 116 bps INFO @ Fri, 10 Dec 2021 23:56:54: #2 alternative fragment length(s) may be 116 bps INFO @ Fri, 10 Dec 2021 23:56:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.20_model.r WARNING @ Fri, 10 Dec 2021 23:56:54: #2 Since the d (116) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 23:56:54: #2 You may need to consider one of the other alternative d(s): 116 WARNING @ Fri, 10 Dec 2021 23:56:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 23:56:54: #3 Call peaks... INFO @ Fri, 10 Dec 2021 23:56:54: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 23:57:26: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 23:57:27: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 23:57:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.20_peaks.xls INFO @ Fri, 10 Dec 2021 23:57:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 23:57:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.20_summits.bed INFO @ Fri, 10 Dec 2021 23:57:42: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1793 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 23:57:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.10_peaks.xls INFO @ Fri, 10 Dec 2021 23:57:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 23:57:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978881/ERX3978881.10_summits.bed INFO @ Fri, 10 Dec 2021 23:57:44: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4501 records, 4 fields): 7 millis CompletedMACS2peakCalling