Job ID = 14168268 SRX = ERX3978844 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 53982737 spots for ERR3975913/ERR3975913.sra Written 53982737 spots for ERR3975913/ERR3975913.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169933 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 04:59:26 53982737 reads; of these: 53982737 (100.00%) were paired; of these: 10066187 (18.65%) aligned concordantly 0 times 23950836 (44.37%) aligned concordantly exactly 1 time 19965714 (36.99%) aligned concordantly >1 times ---- 10066187 pairs aligned concordantly 0 times; of these: 928487 (9.22%) aligned discordantly 1 time ---- 9137700 pairs aligned 0 times concordantly or discordantly; of these: 18275400 mates make up the pairs; of these: 7771528 (42.52%) aligned 0 times 3334787 (18.25%) aligned exactly 1 time 7169085 (39.23%) aligned >1 times 92.80% overall alignment rate Time searching: 04:59:27 Overall time: 04:59:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 15321354 / 44343796 = 0.3455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:20:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:20:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:20:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:20:10: 1000000 INFO @ Fri, 10 Dec 2021 22:20:16: 2000000 INFO @ Fri, 10 Dec 2021 22:20:22: 3000000 INFO @ Fri, 10 Dec 2021 22:20:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:20:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:20:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:20:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:20:34: 5000000 INFO @ Fri, 10 Dec 2021 22:20:42: 6000000 INFO @ Fri, 10 Dec 2021 22:20:42: 1000000 INFO @ Fri, 10 Dec 2021 22:20:49: 7000000 INFO @ Fri, 10 Dec 2021 22:20:51: 2000000 INFO @ Fri, 10 Dec 2021 22:20:57: 8000000 INFO @ Fri, 10 Dec 2021 22:21:00: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:21:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:21:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:21:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:21:04: 9000000 INFO @ Fri, 10 Dec 2021 22:21:09: 4000000 INFO @ Fri, 10 Dec 2021 22:21:11: 1000000 INFO @ Fri, 10 Dec 2021 22:21:12: 10000000 INFO @ Fri, 10 Dec 2021 22:21:18: 5000000 INFO @ Fri, 10 Dec 2021 22:21:19: 2000000 INFO @ Fri, 10 Dec 2021 22:21:19: 11000000 INFO @ Fri, 10 Dec 2021 22:21:26: 3000000 INFO @ Fri, 10 Dec 2021 22:21:27: 6000000 INFO @ Fri, 10 Dec 2021 22:21:27: 12000000 INFO @ Fri, 10 Dec 2021 22:21:34: 4000000 INFO @ Fri, 10 Dec 2021 22:21:34: 13000000 INFO @ Fri, 10 Dec 2021 22:21:35: 7000000 INFO @ Fri, 10 Dec 2021 22:21:42: 5000000 INFO @ Fri, 10 Dec 2021 22:21:42: 14000000 INFO @ Fri, 10 Dec 2021 22:21:44: 8000000 INFO @ Fri, 10 Dec 2021 22:21:49: 6000000 INFO @ Fri, 10 Dec 2021 22:21:50: 15000000 INFO @ Fri, 10 Dec 2021 22:21:53: 9000000 INFO @ Fri, 10 Dec 2021 22:21:57: 7000000 INFO @ Fri, 10 Dec 2021 22:21:57: 16000000 INFO @ Fri, 10 Dec 2021 22:22:02: 10000000 INFO @ Fri, 10 Dec 2021 22:22:05: 8000000 INFO @ Fri, 10 Dec 2021 22:22:05: 17000000 INFO @ Fri, 10 Dec 2021 22:22:11: 11000000 INFO @ Fri, 10 Dec 2021 22:22:12: 9000000 INFO @ Fri, 10 Dec 2021 22:22:13: 18000000 INFO @ Fri, 10 Dec 2021 22:22:19: 12000000 INFO @ Fri, 10 Dec 2021 22:22:20: 10000000 INFO @ Fri, 10 Dec 2021 22:22:20: 19000000 INFO @ Fri, 10 Dec 2021 22:22:27: 11000000 INFO @ Fri, 10 Dec 2021 22:22:28: 20000000 INFO @ Fri, 10 Dec 2021 22:22:28: 13000000 INFO @ Fri, 10 Dec 2021 22:22:35: 12000000 INFO @ Fri, 10 Dec 2021 22:22:35: 21000000 INFO @ Fri, 10 Dec 2021 22:22:37: 14000000 INFO @ Fri, 10 Dec 2021 22:22:43: 13000000 INFO @ Fri, 10 Dec 2021 22:22:43: 22000000 INFO @ Fri, 10 Dec 2021 22:22:46: 15000000 INFO @ Fri, 10 Dec 2021 22:22:50: 14000000 INFO @ Fri, 10 Dec 2021 22:22:51: 23000000 INFO @ Fri, 10 Dec 2021 22:22:55: 16000000 INFO @ Fri, 10 Dec 2021 22:22:58: 15000000 INFO @ Fri, 10 Dec 2021 22:22:58: 24000000 INFO @ Fri, 10 Dec 2021 22:23:03: 17000000 INFO @ Fri, 10 Dec 2021 22:23:05: 16000000 INFO @ Fri, 10 Dec 2021 22:23:06: 25000000 INFO @ Fri, 10 Dec 2021 22:23:12: 18000000 INFO @ Fri, 10 Dec 2021 22:23:13: 17000000 INFO @ Fri, 10 Dec 2021 22:23:13: 26000000 INFO @ Fri, 10 Dec 2021 22:23:21: 18000000 INFO @ Fri, 10 Dec 2021 22:23:21: 27000000 INFO @ Fri, 10 Dec 2021 22:23:21: 19000000 INFO @ Fri, 10 Dec 2021 22:23:28: 19000000 INFO @ Fri, 10 Dec 2021 22:23:29: 28000000 INFO @ Fri, 10 Dec 2021 22:23:30: 20000000 INFO @ Fri, 10 Dec 2021 22:23:36: 20000000 INFO @ Fri, 10 Dec 2021 22:23:36: 29000000 INFO @ Fri, 10 Dec 2021 22:23:39: 21000000 INFO @ Fri, 10 Dec 2021 22:23:44: 21000000 INFO @ Fri, 10 Dec 2021 22:23:44: 30000000 INFO @ Fri, 10 Dec 2021 22:23:48: 22000000 INFO @ Fri, 10 Dec 2021 22:23:51: 22000000 INFO @ Fri, 10 Dec 2021 22:23:52: 31000000 INFO @ Fri, 10 Dec 2021 22:23:56: 23000000 INFO @ Fri, 10 Dec 2021 22:23:59: 23000000 INFO @ Fri, 10 Dec 2021 22:23:59: 32000000 INFO @ Fri, 10 Dec 2021 22:24:05: 24000000 INFO @ Fri, 10 Dec 2021 22:24:07: 24000000 INFO @ Fri, 10 Dec 2021 22:24:07: 33000000 INFO @ Fri, 10 Dec 2021 22:24:14: 25000000 INFO @ Fri, 10 Dec 2021 22:24:14: 34000000 INFO @ Fri, 10 Dec 2021 22:24:15: 25000000 INFO @ Fri, 10 Dec 2021 22:24:22: 35000000 INFO @ Fri, 10 Dec 2021 22:24:23: 26000000 INFO @ Fri, 10 Dec 2021 22:24:24: 26000000 INFO @ Fri, 10 Dec 2021 22:24:29: 36000000 INFO @ Fri, 10 Dec 2021 22:24:33: 27000000 INFO @ Fri, 10 Dec 2021 22:24:33: 27000000 INFO @ Fri, 10 Dec 2021 22:24:37: 37000000 INFO @ Fri, 10 Dec 2021 22:24:42: 28000000 INFO @ Fri, 10 Dec 2021 22:24:43: 28000000 INFO @ Fri, 10 Dec 2021 22:24:44: 38000000 INFO @ Fri, 10 Dec 2021 22:24:50: 29000000 INFO @ Fri, 10 Dec 2021 22:24:52: 29000000 INFO @ Fri, 10 Dec 2021 22:24:52: 39000000 INFO @ Fri, 10 Dec 2021 22:24:58: 30000000 INFO @ Fri, 10 Dec 2021 22:24:59: 40000000 INFO @ Fri, 10 Dec 2021 22:25:00: 30000000 INFO @ Fri, 10 Dec 2021 22:25:05: 31000000 INFO @ Fri, 10 Dec 2021 22:25:07: 41000000 INFO @ Fri, 10 Dec 2021 22:25:09: 31000000 INFO @ Fri, 10 Dec 2021 22:25:13: 32000000 INFO @ Fri, 10 Dec 2021 22:25:15: 42000000 INFO @ Fri, 10 Dec 2021 22:25:18: 32000000 INFO @ Fri, 10 Dec 2021 22:25:20: 33000000 INFO @ Fri, 10 Dec 2021 22:25:22: 43000000 INFO @ Fri, 10 Dec 2021 22:25:27: 33000000 INFO @ Fri, 10 Dec 2021 22:25:28: 34000000 INFO @ Fri, 10 Dec 2021 22:25:30: 44000000 INFO @ Fri, 10 Dec 2021 22:25:35: 34000000 INFO @ Fri, 10 Dec 2021 22:25:36: 35000000 INFO @ Fri, 10 Dec 2021 22:25:38: 45000000 INFO @ Fri, 10 Dec 2021 22:25:43: 36000000 INFO @ Fri, 10 Dec 2021 22:25:44: 35000000 INFO @ Fri, 10 Dec 2021 22:25:45: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 22:25:51: 37000000 INFO @ Fri, 10 Dec 2021 22:25:53: 47000000 INFO @ Fri, 10 Dec 2021 22:25:53: 36000000 INFO @ Fri, 10 Dec 2021 22:25:58: 38000000 INFO @ Fri, 10 Dec 2021 22:26:01: 48000000 INFO @ Fri, 10 Dec 2021 22:26:02: 37000000 INFO @ Fri, 10 Dec 2021 22:26:06: 39000000 INFO @ Fri, 10 Dec 2021 22:26:08: 49000000 INFO @ Fri, 10 Dec 2021 22:26:11: 38000000 INFO @ Fri, 10 Dec 2021 22:26:14: 40000000 INFO @ Fri, 10 Dec 2021 22:26:16: 50000000 INFO @ Fri, 10 Dec 2021 22:26:19: 39000000 INFO @ Fri, 10 Dec 2021 22:26:22: 41000000 INFO @ Fri, 10 Dec 2021 22:26:24: 51000000 INFO @ Fri, 10 Dec 2021 22:26:28: 40000000 INFO @ Fri, 10 Dec 2021 22:26:29: 42000000 INFO @ Fri, 10 Dec 2021 22:26:31: 52000000 INFO @ Fri, 10 Dec 2021 22:26:36: 43000000 INFO @ Fri, 10 Dec 2021 22:26:37: 41000000 INFO @ Fri, 10 Dec 2021 22:26:39: 53000000 INFO @ Fri, 10 Dec 2021 22:26:44: 44000000 INFO @ Fri, 10 Dec 2021 22:26:46: 42000000 INFO @ Fri, 10 Dec 2021 22:26:46: 54000000 INFO @ Fri, 10 Dec 2021 22:26:52: 45000000 INFO @ Fri, 10 Dec 2021 22:26:54: 43000000 INFO @ Fri, 10 Dec 2021 22:26:54: 55000000 INFO @ Fri, 10 Dec 2021 22:26:59: 46000000 INFO @ Fri, 10 Dec 2021 22:27:02: 56000000 INFO @ Fri, 10 Dec 2021 22:27:02: 44000000 INFO @ Fri, 10 Dec 2021 22:27:07: 47000000 INFO @ Fri, 10 Dec 2021 22:27:10: 57000000 INFO @ Fri, 10 Dec 2021 22:27:10: 45000000 INFO @ Fri, 10 Dec 2021 22:27:15: 48000000 INFO @ Fri, 10 Dec 2021 22:27:17: 58000000 INFO @ Fri, 10 Dec 2021 22:27:19: 46000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 22:27:22: 49000000 INFO @ Fri, 10 Dec 2021 22:27:25: 59000000 INFO @ Fri, 10 Dec 2021 22:27:27: 47000000 INFO @ Fri, 10 Dec 2021 22:27:30: 50000000 INFO @ Fri, 10 Dec 2021 22:27:33: 60000000 INFO @ Fri, 10 Dec 2021 22:27:35: 48000000 INFO @ Fri, 10 Dec 2021 22:27:38: 51000000 INFO @ Fri, 10 Dec 2021 22:27:40: 61000000 INFO @ Fri, 10 Dec 2021 22:27:44: 49000000 INFO @ Fri, 10 Dec 2021 22:27:45: 52000000 INFO @ Fri, 10 Dec 2021 22:27:48: 62000000 INFO @ Fri, 10 Dec 2021 22:27:52: 50000000 INFO @ Fri, 10 Dec 2021 22:27:53: 53000000 INFO @ Fri, 10 Dec 2021 22:27:56: 63000000 INFO @ Fri, 10 Dec 2021 22:28:00: 54000000 INFO @ Fri, 10 Dec 2021 22:28:01: 51000000 INFO @ Fri, 10 Dec 2021 22:28:03: 64000000 INFO @ Fri, 10 Dec 2021 22:28:08: 55000000 INFO @ Fri, 10 Dec 2021 22:28:09: 52000000 INFO @ Fri, 10 Dec 2021 22:28:11: 65000000 INFO @ Fri, 10 Dec 2021 22:28:16: 56000000 INFO @ Fri, 10 Dec 2021 22:28:17: 53000000 INFO @ Fri, 10 Dec 2021 22:28:19: 66000000 INFO @ Fri, 10 Dec 2021 22:28:24: 57000000 INFO @ Fri, 10 Dec 2021 22:28:26: 54000000 INFO @ Fri, 10 Dec 2021 22:28:26: 67000000 INFO @ Fri, 10 Dec 2021 22:28:31: 58000000 INFO @ Fri, 10 Dec 2021 22:28:34: 68000000 INFO @ Fri, 10 Dec 2021 22:28:34: 55000000 INFO @ Fri, 10 Dec 2021 22:28:39: 59000000 INFO @ Fri, 10 Dec 2021 22:28:41: 69000000 INFO @ Fri, 10 Dec 2021 22:28:43: 56000000 INFO @ Fri, 10 Dec 2021 22:28:46: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 22:28:46: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 22:28:46: #1 total tags in treatment: 28733285 INFO @ Fri, 10 Dec 2021 22:28:46: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:28:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:28:46: #1 tags after filtering in treatment: 23946985 INFO @ Fri, 10 Dec 2021 22:28:46: #1 Redundant rate of treatment: 0.17 INFO @ Fri, 10 Dec 2021 22:28:46: #1 finished! INFO @ Fri, 10 Dec 2021 22:28:46: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:28:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:28:47: 60000000 INFO @ Fri, 10 Dec 2021 22:28:48: #2 number of paired peaks: 143 WARNING @ Fri, 10 Dec 2021 22:28:48: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Fri, 10 Dec 2021 22:28:48: start model_add_line... INFO @ Fri, 10 Dec 2021 22:28:48: start X-correlation... INFO @ Fri, 10 Dec 2021 22:28:48: end of X-cor INFO @ Fri, 10 Dec 2021 22:28:48: #2 finished! INFO @ Fri, 10 Dec 2021 22:28:48: #2 predicted fragment length is 109 bps INFO @ Fri, 10 Dec 2021 22:28:48: #2 alternative fragment length(s) may be 109 bps INFO @ Fri, 10 Dec 2021 22:28:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.05_model.r WARNING @ Fri, 10 Dec 2021 22:28:48: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:28:48: #2 You may need to consider one of the other alternative d(s): 109 WARNING @ Fri, 10 Dec 2021 22:28:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:28:48: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:28:48: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:28:51: 57000000 INFO @ Fri, 10 Dec 2021 22:28:54: 61000000 INFO @ Fri, 10 Dec 2021 22:29:00: 58000000 INFO @ Fri, 10 Dec 2021 22:29:02: 62000000 INFO @ Fri, 10 Dec 2021 22:29:08: 59000000 INFO @ Fri, 10 Dec 2021 22:29:09: 63000000 INFO @ Fri, 10 Dec 2021 22:29:16: 60000000 INFO @ Fri, 10 Dec 2021 22:29:17: 64000000 INFO @ Fri, 10 Dec 2021 22:29:24: 61000000 INFO @ Fri, 10 Dec 2021 22:29:24: 65000000 INFO @ Fri, 10 Dec 2021 22:29:28: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:29:32: 66000000 INFO @ Fri, 10 Dec 2021 22:29:32: 62000000 INFO @ Fri, 10 Dec 2021 22:29:39: 67000000 INFO @ Fri, 10 Dec 2021 22:29:41: 63000000 INFO @ Fri, 10 Dec 2021 22:29:47: 68000000 INFO @ Fri, 10 Dec 2021 22:29:49: 64000000 INFO @ Fri, 10 Dec 2021 22:29:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.05_peaks.xls INFO @ Fri, 10 Dec 2021 22:29:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:29:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.05_summits.bed INFO @ Fri, 10 Dec 2021 22:29:50: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (9351 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:29:54: 69000000 INFO @ Fri, 10 Dec 2021 22:29:58: 65000000 INFO @ Fri, 10 Dec 2021 22:29:59: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 22:29:59: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 22:29:59: #1 total tags in treatment: 28733285 INFO @ Fri, 10 Dec 2021 22:29:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:29:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:29:59: #1 tags after filtering in treatment: 23946985 INFO @ Fri, 10 Dec 2021 22:29:59: #1 Redundant rate of treatment: 0.17 INFO @ Fri, 10 Dec 2021 22:29:59: #1 finished! INFO @ Fri, 10 Dec 2021 22:29:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:29:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:30:01: #2 number of paired peaks: 143 WARNING @ Fri, 10 Dec 2021 22:30:01: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Fri, 10 Dec 2021 22:30:01: start model_add_line... INFO @ Fri, 10 Dec 2021 22:30:01: start X-correlation... INFO @ Fri, 10 Dec 2021 22:30:01: end of X-cor INFO @ Fri, 10 Dec 2021 22:30:01: #2 finished! INFO @ Fri, 10 Dec 2021 22:30:01: #2 predicted fragment length is 109 bps INFO @ Fri, 10 Dec 2021 22:30:01: #2 alternative fragment length(s) may be 109 bps INFO @ Fri, 10 Dec 2021 22:30:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.20_model.r WARNING @ Fri, 10 Dec 2021 22:30:01: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:30:01: #2 You may need to consider one of the other alternative d(s): 109 WARNING @ Fri, 10 Dec 2021 22:30:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:30:01: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:30:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:30:05: 66000000 INFO @ Fri, 10 Dec 2021 22:30:13: 67000000 INFO @ Fri, 10 Dec 2021 22:30:20: 68000000 INFO @ Fri, 10 Dec 2021 22:30:28: 69000000 INFO @ Fri, 10 Dec 2021 22:30:32: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 22:30:32: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 22:30:32: #1 total tags in treatment: 28733285 INFO @ Fri, 10 Dec 2021 22:30:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:30:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:30:33: #1 tags after filtering in treatment: 23946985 INFO @ Fri, 10 Dec 2021 22:30:33: #1 Redundant rate of treatment: 0.17 INFO @ Fri, 10 Dec 2021 22:30:33: #1 finished! INFO @ Fri, 10 Dec 2021 22:30:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:30:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:30:34: #2 number of paired peaks: 143 WARNING @ Fri, 10 Dec 2021 22:30:34: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Fri, 10 Dec 2021 22:30:34: start model_add_line... INFO @ Fri, 10 Dec 2021 22:30:34: start X-correlation... INFO @ Fri, 10 Dec 2021 22:30:34: end of X-cor INFO @ Fri, 10 Dec 2021 22:30:34: #2 finished! INFO @ Fri, 10 Dec 2021 22:30:34: #2 predicted fragment length is 109 bps INFO @ Fri, 10 Dec 2021 22:30:34: #2 alternative fragment length(s) may be 109 bps INFO @ Fri, 10 Dec 2021 22:30:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.10_model.r WARNING @ Fri, 10 Dec 2021 22:30:34: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:30:34: #2 You may need to consider one of the other alternative d(s): 109 WARNING @ Fri, 10 Dec 2021 22:30:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:30:34: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:30:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:30:42: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:31:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.20_peaks.xls INFO @ Fri, 10 Dec 2021 22:31:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:31:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.20_summits.bed INFO @ Fri, 10 Dec 2021 22:31:03: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (2332 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:31:15: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:31:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.10_peaks.xls INFO @ Fri, 10 Dec 2021 22:31:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:31:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978844/ERX3978844.10_summits.bed INFO @ Fri, 10 Dec 2021 22:31:36: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (5640 records, 4 fields): 7 millis CompletedMACS2peakCalling