Job ID = 14168031 SRX = ERX3978818 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 23272029 spots for ERR3975865/ERR3975865.sra Written 23272029 spots for ERR3975865/ERR3975865.sra Read 23004617 spots for ERR3975872/ERR3975872.sra Written 23004617 spots for ERR3975872/ERR3975872.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169322 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:43:18 46276646 reads; of these: 46276646 (100.00%) were paired; of these: 11906622 (25.73%) aligned concordantly 0 times 26044032 (56.28%) aligned concordantly exactly 1 time 8325992 (17.99%) aligned concordantly >1 times ---- 11906622 pairs aligned concordantly 0 times; of these: 5174485 (43.46%) aligned discordantly 1 time ---- 6732137 pairs aligned 0 times concordantly or discordantly; of these: 13464274 mates make up the pairs; of these: 8901891 (66.11%) aligned 0 times 1130700 (8.40%) aligned exactly 1 time 3431683 (25.49%) aligned >1 times 90.38% overall alignment rate Time searching: 02:43:19 Overall time: 02:43:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4865442 / 39103428 = 0.1244 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:04:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:04:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:04:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:04:37: 1000000 INFO @ Fri, 10 Dec 2021 18:04:48: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:04:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:04:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:04:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:05:01: 3000000 INFO @ Fri, 10 Dec 2021 18:05:07: 1000000 INFO @ Fri, 10 Dec 2021 18:05:13: 4000000 INFO @ Fri, 10 Dec 2021 18:05:20: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:05:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:05:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:05:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:05:25: 5000000 INFO @ Fri, 10 Dec 2021 18:05:32: 3000000 INFO @ Fri, 10 Dec 2021 18:05:37: 1000000 INFO @ Fri, 10 Dec 2021 18:05:38: 6000000 INFO @ Fri, 10 Dec 2021 18:05:44: 4000000 INFO @ Fri, 10 Dec 2021 18:05:50: 2000000 INFO @ Fri, 10 Dec 2021 18:05:50: 7000000 INFO @ Fri, 10 Dec 2021 18:05:55: 5000000 INFO @ Fri, 10 Dec 2021 18:06:02: 3000000 INFO @ Fri, 10 Dec 2021 18:06:03: 8000000 INFO @ Fri, 10 Dec 2021 18:06:07: 6000000 INFO @ Fri, 10 Dec 2021 18:06:15: 4000000 INFO @ Fri, 10 Dec 2021 18:06:15: 9000000 INFO @ Fri, 10 Dec 2021 18:06:19: 7000000 INFO @ Fri, 10 Dec 2021 18:06:27: 5000000 INFO @ Fri, 10 Dec 2021 18:06:27: 10000000 INFO @ Fri, 10 Dec 2021 18:06:31: 8000000 INFO @ Fri, 10 Dec 2021 18:06:40: 6000000 INFO @ Fri, 10 Dec 2021 18:06:40: 11000000 INFO @ Fri, 10 Dec 2021 18:06:42: 9000000 INFO @ Fri, 10 Dec 2021 18:06:52: 12000000 INFO @ Fri, 10 Dec 2021 18:06:52: 7000000 INFO @ Fri, 10 Dec 2021 18:06:54: 10000000 INFO @ Fri, 10 Dec 2021 18:07:04: 13000000 INFO @ Fri, 10 Dec 2021 18:07:05: 8000000 INFO @ Fri, 10 Dec 2021 18:07:06: 11000000 INFO @ Fri, 10 Dec 2021 18:07:16: 14000000 INFO @ Fri, 10 Dec 2021 18:07:17: 9000000 INFO @ Fri, 10 Dec 2021 18:07:18: 12000000 INFO @ Fri, 10 Dec 2021 18:07:28: 15000000 INFO @ Fri, 10 Dec 2021 18:07:29: 10000000 INFO @ Fri, 10 Dec 2021 18:07:30: 13000000 INFO @ Fri, 10 Dec 2021 18:07:40: 16000000 INFO @ Fri, 10 Dec 2021 18:07:42: 14000000 INFO @ Fri, 10 Dec 2021 18:07:42: 11000000 INFO @ Fri, 10 Dec 2021 18:07:53: 17000000 INFO @ Fri, 10 Dec 2021 18:07:53: 15000000 INFO @ Fri, 10 Dec 2021 18:07:54: 12000000 INFO @ Fri, 10 Dec 2021 18:08:05: 16000000 INFO @ Fri, 10 Dec 2021 18:08:05: 18000000 INFO @ Fri, 10 Dec 2021 18:08:07: 13000000 INFO @ Fri, 10 Dec 2021 18:08:17: 17000000 INFO @ Fri, 10 Dec 2021 18:08:17: 19000000 INFO @ Fri, 10 Dec 2021 18:08:19: 14000000 INFO @ Fri, 10 Dec 2021 18:08:29: 18000000 INFO @ Fri, 10 Dec 2021 18:08:30: 20000000 INFO @ Fri, 10 Dec 2021 18:08:31: 15000000 INFO @ Fri, 10 Dec 2021 18:08:41: 19000000 INFO @ Fri, 10 Dec 2021 18:08:42: 21000000 INFO @ Fri, 10 Dec 2021 18:08:44: 16000000 INFO @ Fri, 10 Dec 2021 18:08:52: 20000000 INFO @ Fri, 10 Dec 2021 18:08:55: 22000000 INFO @ Fri, 10 Dec 2021 18:08:57: 17000000 INFO @ Fri, 10 Dec 2021 18:09:04: 21000000 INFO @ Fri, 10 Dec 2021 18:09:07: 23000000 INFO @ Fri, 10 Dec 2021 18:09:09: 18000000 INFO @ Fri, 10 Dec 2021 18:09:17: 22000000 INFO @ Fri, 10 Dec 2021 18:09:20: 24000000 INFO @ Fri, 10 Dec 2021 18:09:22: 19000000 INFO @ Fri, 10 Dec 2021 18:09:29: 23000000 INFO @ Fri, 10 Dec 2021 18:09:33: 25000000 INFO @ Fri, 10 Dec 2021 18:09:34: 20000000 INFO @ Fri, 10 Dec 2021 18:09:41: 24000000 INFO @ Fri, 10 Dec 2021 18:09:45: 26000000 INFO @ Fri, 10 Dec 2021 18:09:47: 21000000 INFO @ Fri, 10 Dec 2021 18:09:54: 25000000 INFO @ Fri, 10 Dec 2021 18:09:58: 27000000 INFO @ Fri, 10 Dec 2021 18:10:00: 22000000 INFO @ Fri, 10 Dec 2021 18:10:06: 26000000 INFO @ Fri, 10 Dec 2021 18:10:10: 28000000 INFO @ Fri, 10 Dec 2021 18:10:13: 23000000 INFO @ Fri, 10 Dec 2021 18:10:18: 27000000 INFO @ Fri, 10 Dec 2021 18:10:23: 29000000 INFO @ Fri, 10 Dec 2021 18:10:25: 24000000 INFO @ Fri, 10 Dec 2021 18:10:30: 28000000 INFO @ Fri, 10 Dec 2021 18:10:35: 30000000 INFO @ Fri, 10 Dec 2021 18:10:38: 25000000 INFO @ Fri, 10 Dec 2021 18:10:42: 29000000 INFO @ Fri, 10 Dec 2021 18:10:48: 31000000 INFO @ Fri, 10 Dec 2021 18:10:50: 26000000 INFO @ Fri, 10 Dec 2021 18:10:54: 30000000 INFO @ Fri, 10 Dec 2021 18:11:00: 32000000 INFO @ Fri, 10 Dec 2021 18:11:03: 27000000 INFO @ Fri, 10 Dec 2021 18:11:06: 31000000 INFO @ Fri, 10 Dec 2021 18:11:12: 33000000 INFO @ Fri, 10 Dec 2021 18:11:16: 28000000 INFO @ Fri, 10 Dec 2021 18:11:18: 32000000 INFO @ Fri, 10 Dec 2021 18:11:24: 34000000 INFO @ Fri, 10 Dec 2021 18:11:28: 29000000 INFO @ Fri, 10 Dec 2021 18:11:30: 33000000 INFO @ Fri, 10 Dec 2021 18:11:36: 35000000 INFO @ Fri, 10 Dec 2021 18:11:40: 30000000 INFO @ Fri, 10 Dec 2021 18:11:42: 34000000 INFO @ Fri, 10 Dec 2021 18:11:48: 36000000 INFO @ Fri, 10 Dec 2021 18:11:53: 31000000 INFO @ Fri, 10 Dec 2021 18:11:53: 35000000 INFO @ Fri, 10 Dec 2021 18:11:59: 37000000 INFO @ Fri, 10 Dec 2021 18:12:05: 36000000 INFO @ Fri, 10 Dec 2021 18:12:06: 32000000 INFO @ Fri, 10 Dec 2021 18:12:11: 38000000 INFO @ Fri, 10 Dec 2021 18:12:16: 37000000 INFO @ Fri, 10 Dec 2021 18:12:18: 33000000 INFO @ Fri, 10 Dec 2021 18:12:23: 39000000 INFO @ Fri, 10 Dec 2021 18:12:28: 38000000 INFO @ Fri, 10 Dec 2021 18:12:31: 34000000 INFO @ Fri, 10 Dec 2021 18:12:35: 40000000 INFO @ Fri, 10 Dec 2021 18:12:39: 39000000 INFO @ Fri, 10 Dec 2021 18:12:43: 35000000 INFO @ Fri, 10 Dec 2021 18:12:47: 41000000 INFO @ Fri, 10 Dec 2021 18:12:51: 40000000 INFO @ Fri, 10 Dec 2021 18:12:55: 36000000 INFO @ Fri, 10 Dec 2021 18:12:59: 42000000 INFO @ Fri, 10 Dec 2021 18:13:03: 41000000 INFO @ Fri, 10 Dec 2021 18:13:07: 37000000 INFO @ Fri, 10 Dec 2021 18:13:11: 43000000 INFO @ Fri, 10 Dec 2021 18:13:14: 42000000 INFO @ Fri, 10 Dec 2021 18:13:19: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 18:13:23: 44000000 INFO @ Fri, 10 Dec 2021 18:13:26: 43000000 INFO @ Fri, 10 Dec 2021 18:13:32: 39000000 INFO @ Fri, 10 Dec 2021 18:13:34: 45000000 INFO @ Fri, 10 Dec 2021 18:13:38: 44000000 INFO @ Fri, 10 Dec 2021 18:13:44: 40000000 INFO @ Fri, 10 Dec 2021 18:13:45: 46000000 INFO @ Fri, 10 Dec 2021 18:13:49: 45000000 INFO @ Fri, 10 Dec 2021 18:13:56: 41000000 INFO @ Fri, 10 Dec 2021 18:13:56: 47000000 INFO @ Fri, 10 Dec 2021 18:14:00: 46000000 INFO @ Fri, 10 Dec 2021 18:14:07: 48000000 INFO @ Fri, 10 Dec 2021 18:14:08: 42000000 INFO @ Fri, 10 Dec 2021 18:14:10: 47000000 INFO @ Fri, 10 Dec 2021 18:14:18: 49000000 INFO @ Fri, 10 Dec 2021 18:14:21: 43000000 INFO @ Fri, 10 Dec 2021 18:14:21: 48000000 INFO @ Fri, 10 Dec 2021 18:14:29: 50000000 INFO @ Fri, 10 Dec 2021 18:14:32: 49000000 INFO @ Fri, 10 Dec 2021 18:14:33: 44000000 INFO @ Fri, 10 Dec 2021 18:14:40: 51000000 INFO @ Fri, 10 Dec 2021 18:14:42: 50000000 INFO @ Fri, 10 Dec 2021 18:14:45: 45000000 INFO @ Fri, 10 Dec 2021 18:14:51: 52000000 INFO @ Fri, 10 Dec 2021 18:14:53: 51000000 INFO @ Fri, 10 Dec 2021 18:14:56: 46000000 INFO @ Fri, 10 Dec 2021 18:15:02: 53000000 INFO @ Fri, 10 Dec 2021 18:15:03: 52000000 INFO @ Fri, 10 Dec 2021 18:15:08: 47000000 INFO @ Fri, 10 Dec 2021 18:15:13: 54000000 INFO @ Fri, 10 Dec 2021 18:15:14: 53000000 INFO @ Fri, 10 Dec 2021 18:15:19: 48000000 INFO @ Fri, 10 Dec 2021 18:15:23: 55000000 INFO @ Fri, 10 Dec 2021 18:15:24: 54000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 18:15:30: 49000000 INFO @ Fri, 10 Dec 2021 18:15:34: 56000000 INFO @ Fri, 10 Dec 2021 18:15:35: 55000000 INFO @ Fri, 10 Dec 2021 18:15:41: 50000000 INFO @ Fri, 10 Dec 2021 18:15:45: 57000000 INFO @ Fri, 10 Dec 2021 18:15:45: 56000000 INFO @ Fri, 10 Dec 2021 18:15:53: 51000000 INFO @ Fri, 10 Dec 2021 18:15:56: 57000000 INFO @ Fri, 10 Dec 2021 18:15:56: 58000000 INFO @ Fri, 10 Dec 2021 18:16:04: 52000000 INFO @ Fri, 10 Dec 2021 18:16:07: 58000000 INFO @ Fri, 10 Dec 2021 18:16:08: 59000000 INFO @ Fri, 10 Dec 2021 18:16:15: 53000000 INFO @ Fri, 10 Dec 2021 18:16:17: 59000000 INFO @ Fri, 10 Dec 2021 18:16:19: 60000000 INFO @ Fri, 10 Dec 2021 18:16:26: 54000000 INFO @ Fri, 10 Dec 2021 18:16:28: 60000000 INFO @ Fri, 10 Dec 2021 18:16:30: 61000000 INFO @ Fri, 10 Dec 2021 18:16:37: 55000000 INFO @ Fri, 10 Dec 2021 18:16:38: 61000000 INFO @ Fri, 10 Dec 2021 18:16:40: 62000000 INFO @ Fri, 10 Dec 2021 18:16:48: 56000000 INFO @ Fri, 10 Dec 2021 18:16:49: 62000000 INFO @ Fri, 10 Dec 2021 18:16:51: 63000000 INFO @ Fri, 10 Dec 2021 18:16:59: 57000000 INFO @ Fri, 10 Dec 2021 18:17:00: 63000000 INFO @ Fri, 10 Dec 2021 18:17:02: 64000000 INFO @ Fri, 10 Dec 2021 18:17:10: 58000000 INFO @ Fri, 10 Dec 2021 18:17:10: 64000000 INFO @ Fri, 10 Dec 2021 18:17:13: 65000000 INFO @ Fri, 10 Dec 2021 18:17:21: 65000000 INFO @ Fri, 10 Dec 2021 18:17:22: 59000000 INFO @ Fri, 10 Dec 2021 18:17:24: 66000000 INFO @ Fri, 10 Dec 2021 18:17:32: 66000000 INFO @ Fri, 10 Dec 2021 18:17:33: 60000000 INFO @ Fri, 10 Dec 2021 18:17:35: 67000000 INFO @ Fri, 10 Dec 2021 18:17:42: 67000000 INFO @ Fri, 10 Dec 2021 18:17:44: 61000000 INFO @ Fri, 10 Dec 2021 18:17:46: 68000000 INFO @ Fri, 10 Dec 2021 18:17:53: 68000000 INFO @ Fri, 10 Dec 2021 18:17:55: 62000000 INFO @ Fri, 10 Dec 2021 18:17:57: 69000000 INFO @ Fri, 10 Dec 2021 18:18:04: 69000000 INFO @ Fri, 10 Dec 2021 18:18:06: 63000000 INFO @ Fri, 10 Dec 2021 18:18:08: 70000000 INFO @ Fri, 10 Dec 2021 18:18:14: 70000000 INFO @ Fri, 10 Dec 2021 18:18:18: 64000000 INFO @ Fri, 10 Dec 2021 18:18:19: 71000000 INFO @ Fri, 10 Dec 2021 18:18:25: 71000000 INFO @ Fri, 10 Dec 2021 18:18:29: 65000000 INFO @ Fri, 10 Dec 2021 18:18:29: 72000000 INFO @ Fri, 10 Dec 2021 18:18:36: 72000000 INFO @ Fri, 10 Dec 2021 18:18:40: 66000000 INFO @ Fri, 10 Dec 2021 18:18:40: 73000000 INFO @ Fri, 10 Dec 2021 18:18:46: 73000000 INFO @ Fri, 10 Dec 2021 18:18:50: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 18:18:50: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 18:18:50: #1 total tags in treatment: 30035350 INFO @ Fri, 10 Dec 2021 18:18:50: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:18:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:18:51: 67000000 INFO @ Fri, 10 Dec 2021 18:18:51: #1 tags after filtering in treatment: 23354920 INFO @ Fri, 10 Dec 2021 18:18:51: #1 Redundant rate of treatment: 0.22 INFO @ Fri, 10 Dec 2021 18:18:51: #1 finished! INFO @ Fri, 10 Dec 2021 18:18:51: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:18:53: #2 number of paired peaks: 229 WARNING @ Fri, 10 Dec 2021 18:18:53: Fewer paired peaks (229) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 229 pairs to build model! INFO @ Fri, 10 Dec 2021 18:18:53: start model_add_line... INFO @ Fri, 10 Dec 2021 18:18:53: start X-correlation... INFO @ Fri, 10 Dec 2021 18:18:53: end of X-cor INFO @ Fri, 10 Dec 2021 18:18:53: #2 finished! INFO @ Fri, 10 Dec 2021 18:18:53: #2 predicted fragment length is 101 bps INFO @ Fri, 10 Dec 2021 18:18:53: #2 alternative fragment length(s) may be 4,101 bps INFO @ Fri, 10 Dec 2021 18:18:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.05_model.r WARNING @ Fri, 10 Dec 2021 18:18:53: #2 Since the d (101) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 18:18:53: #2 You may need to consider one of the other alternative d(s): 4,101 WARNING @ Fri, 10 Dec 2021 18:18:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 18:18:53: #3 Call peaks... INFO @ Fri, 10 Dec 2021 18:18:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 18:18:56: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 18:18:56: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 18:18:56: #1 total tags in treatment: 30035350 INFO @ Fri, 10 Dec 2021 18:18:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:18:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:18:57: #1 tags after filtering in treatment: 23354920 INFO @ Fri, 10 Dec 2021 18:18:57: #1 Redundant rate of treatment: 0.22 INFO @ Fri, 10 Dec 2021 18:18:57: #1 finished! INFO @ Fri, 10 Dec 2021 18:18:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:18:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:18:59: #2 number of paired peaks: 229 WARNING @ Fri, 10 Dec 2021 18:18:59: Fewer paired peaks (229) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 229 pairs to build model! INFO @ Fri, 10 Dec 2021 18:18:59: start model_add_line... INFO @ Fri, 10 Dec 2021 18:18:59: start X-correlation... INFO @ Fri, 10 Dec 2021 18:18:59: end of X-cor INFO @ Fri, 10 Dec 2021 18:18:59: #2 finished! INFO @ Fri, 10 Dec 2021 18:18:59: #2 predicted fragment length is 101 bps INFO @ Fri, 10 Dec 2021 18:18:59: #2 alternative fragment length(s) may be 4,101 bps INFO @ Fri, 10 Dec 2021 18:18:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.10_model.r WARNING @ Fri, 10 Dec 2021 18:18:59: #2 Since the d (101) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 18:18:59: #2 You may need to consider one of the other alternative d(s): 4,101 WARNING @ Fri, 10 Dec 2021 18:18:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 18:18:59: #3 Call peaks... INFO @ Fri, 10 Dec 2021 18:18:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 18:19:01: 68000000 INFO @ Fri, 10 Dec 2021 18:19:12: 69000000 INFO @ Fri, 10 Dec 2021 18:19:23: 70000000 INFO @ Fri, 10 Dec 2021 18:19:34: 71000000 INFO @ Fri, 10 Dec 2021 18:19:44: 72000000 INFO @ Fri, 10 Dec 2021 18:19:52: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 18:19:55: 73000000 INFO @ Fri, 10 Dec 2021 18:20:00: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 18:20:06: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 18:20:06: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 18:20:06: #1 total tags in treatment: 30035350 INFO @ Fri, 10 Dec 2021 18:20:06: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:20:06: #1 tags after filtering in treatment: 23354920 INFO @ Fri, 10 Dec 2021 18:20:06: #1 Redundant rate of treatment: 0.22 INFO @ Fri, 10 Dec 2021 18:20:06: #1 finished! INFO @ Fri, 10 Dec 2021 18:20:06: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:20:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:20:08: #2 number of paired peaks: 229 WARNING @ Fri, 10 Dec 2021 18:20:08: Fewer paired peaks (229) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 229 pairs to build model! INFO @ Fri, 10 Dec 2021 18:20:08: start model_add_line... INFO @ Fri, 10 Dec 2021 18:20:08: start X-correlation... INFO @ Fri, 10 Dec 2021 18:20:08: end of X-cor INFO @ Fri, 10 Dec 2021 18:20:08: #2 finished! INFO @ Fri, 10 Dec 2021 18:20:08: #2 predicted fragment length is 101 bps INFO @ Fri, 10 Dec 2021 18:20:08: #2 alternative fragment length(s) may be 4,101 bps INFO @ Fri, 10 Dec 2021 18:20:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.20_model.r WARNING @ Fri, 10 Dec 2021 18:20:09: #2 Since the d (101) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 18:20:09: #2 You may need to consider one of the other alternative d(s): 4,101 WARNING @ Fri, 10 Dec 2021 18:20:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 18:20:09: #3 Call peaks... INFO @ Fri, 10 Dec 2021 18:20:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 18:20:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.05_peaks.xls INFO @ Fri, 10 Dec 2021 18:20:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 18:20:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.05_summits.bed INFO @ Fri, 10 Dec 2021 18:20:21: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (11625 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:20:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.10_peaks.xls INFO @ Fri, 10 Dec 2021 18:20:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 18:20:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.10_summits.bed INFO @ Fri, 10 Dec 2021 18:20:31: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (7823 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:21:08: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 18:21:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.20_peaks.xls INFO @ Fri, 10 Dec 2021 18:21:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 18:21:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978818/ERX3978818.20_summits.bed INFO @ Fri, 10 Dec 2021 18:21:36: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (2890 records, 4 fields): 10 millis CompletedMACS2peakCalling