Job ID = 14168030 SRX = ERX3978817 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18240205 spots for ERR3975862/ERR3975862.sra Written 18240205 spots for ERR3975862/ERR3975862.sra Read 23012450 spots for ERR3975871/ERR3975871.sra Written 23012450 spots for ERR3975871/ERR3975871.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169020 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:37:04 41252655 reads; of these: 41252655 (100.00%) were paired; of these: 5603548 (13.58%) aligned concordantly 0 times 29051571 (70.42%) aligned concordantly exactly 1 time 6597536 (15.99%) aligned concordantly >1 times ---- 5603548 pairs aligned concordantly 0 times; of these: 2220395 (39.62%) aligned discordantly 1 time ---- 3383153 pairs aligned 0 times concordantly or discordantly; of these: 6766306 mates make up the pairs; of these: 4541613 (67.12%) aligned 0 times 1173534 (17.34%) aligned exactly 1 time 1051159 (15.54%) aligned >1 times 94.50% overall alignment rate Time searching: 01:37:05 Overall time: 01:37:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8436821 / 37667813 = 0.2240 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:41:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:41:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:41:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:41:37: 1000000 INFO @ Fri, 10 Dec 2021 16:41:46: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:41:56: 3000000 INFO @ Fri, 10 Dec 2021 16:41:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:41:57: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:41:57: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:42:07: 4000000 INFO @ Fri, 10 Dec 2021 16:42:08: 1000000 INFO @ Fri, 10 Dec 2021 16:42:18: 5000000 INFO @ Fri, 10 Dec 2021 16:42:18: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:42:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:42:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:42:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:42:29: 6000000 INFO @ Fri, 10 Dec 2021 16:42:29: 3000000 INFO @ Fri, 10 Dec 2021 16:42:38: 1000000 INFO @ Fri, 10 Dec 2021 16:42:39: 7000000 INFO @ Fri, 10 Dec 2021 16:42:39: 4000000 INFO @ Fri, 10 Dec 2021 16:42:48: 2000000 INFO @ Fri, 10 Dec 2021 16:42:50: 5000000 INFO @ Fri, 10 Dec 2021 16:42:50: 8000000 INFO @ Fri, 10 Dec 2021 16:42:59: 3000000 INFO @ Fri, 10 Dec 2021 16:43:01: 6000000 INFO @ Fri, 10 Dec 2021 16:43:01: 9000000 INFO @ Fri, 10 Dec 2021 16:43:10: 4000000 INFO @ Fri, 10 Dec 2021 16:43:12: 7000000 INFO @ Fri, 10 Dec 2021 16:43:12: 10000000 INFO @ Fri, 10 Dec 2021 16:43:20: 5000000 INFO @ Fri, 10 Dec 2021 16:43:23: 8000000 INFO @ Fri, 10 Dec 2021 16:43:23: 11000000 INFO @ Fri, 10 Dec 2021 16:43:31: 6000000 INFO @ Fri, 10 Dec 2021 16:43:34: 9000000 INFO @ Fri, 10 Dec 2021 16:43:34: 12000000 INFO @ Fri, 10 Dec 2021 16:43:42: 7000000 INFO @ Fri, 10 Dec 2021 16:43:45: 10000000 INFO @ Fri, 10 Dec 2021 16:43:46: 13000000 INFO @ Fri, 10 Dec 2021 16:43:52: 8000000 INFO @ Fri, 10 Dec 2021 16:43:55: 11000000 INFO @ Fri, 10 Dec 2021 16:43:57: 14000000 INFO @ Fri, 10 Dec 2021 16:44:03: 9000000 INFO @ Fri, 10 Dec 2021 16:44:06: 12000000 INFO @ Fri, 10 Dec 2021 16:44:08: 15000000 INFO @ Fri, 10 Dec 2021 16:44:14: 10000000 INFO @ Fri, 10 Dec 2021 16:44:17: 13000000 INFO @ Fri, 10 Dec 2021 16:44:19: 16000000 INFO @ Fri, 10 Dec 2021 16:44:24: 11000000 INFO @ Fri, 10 Dec 2021 16:44:28: 14000000 INFO @ Fri, 10 Dec 2021 16:44:30: 17000000 INFO @ Fri, 10 Dec 2021 16:44:35: 12000000 INFO @ Fri, 10 Dec 2021 16:44:39: 15000000 INFO @ Fri, 10 Dec 2021 16:44:41: 18000000 INFO @ Fri, 10 Dec 2021 16:44:45: 13000000 INFO @ Fri, 10 Dec 2021 16:44:50: 16000000 INFO @ Fri, 10 Dec 2021 16:44:52: 19000000 INFO @ Fri, 10 Dec 2021 16:44:56: 14000000 INFO @ Fri, 10 Dec 2021 16:45:01: 17000000 INFO @ Fri, 10 Dec 2021 16:45:04: 20000000 INFO @ Fri, 10 Dec 2021 16:45:07: 15000000 INFO @ Fri, 10 Dec 2021 16:45:12: 18000000 INFO @ Fri, 10 Dec 2021 16:45:15: 21000000 INFO @ Fri, 10 Dec 2021 16:45:17: 16000000 INFO @ Fri, 10 Dec 2021 16:45:23: 19000000 INFO @ Fri, 10 Dec 2021 16:45:26: 22000000 INFO @ Fri, 10 Dec 2021 16:45:28: 17000000 INFO @ Fri, 10 Dec 2021 16:45:34: 20000000 INFO @ Fri, 10 Dec 2021 16:45:37: 23000000 INFO @ Fri, 10 Dec 2021 16:45:39: 18000000 INFO @ Fri, 10 Dec 2021 16:45:45: 21000000 INFO @ Fri, 10 Dec 2021 16:45:48: 24000000 INFO @ Fri, 10 Dec 2021 16:45:50: 19000000 INFO @ Fri, 10 Dec 2021 16:45:56: 22000000 INFO @ Fri, 10 Dec 2021 16:45:59: 25000000 INFO @ Fri, 10 Dec 2021 16:46:01: 20000000 INFO @ Fri, 10 Dec 2021 16:46:07: 23000000 INFO @ Fri, 10 Dec 2021 16:46:10: 26000000 INFO @ Fri, 10 Dec 2021 16:46:12: 21000000 INFO @ Fri, 10 Dec 2021 16:46:18: 24000000 INFO @ Fri, 10 Dec 2021 16:46:21: 27000000 INFO @ Fri, 10 Dec 2021 16:46:22: 22000000 INFO @ Fri, 10 Dec 2021 16:46:29: 25000000 INFO @ Fri, 10 Dec 2021 16:46:32: 28000000 INFO @ Fri, 10 Dec 2021 16:46:33: 23000000 INFO @ Fri, 10 Dec 2021 16:46:40: 26000000 INFO @ Fri, 10 Dec 2021 16:46:43: 29000000 INFO @ Fri, 10 Dec 2021 16:46:44: 24000000 INFO @ Fri, 10 Dec 2021 16:46:51: 27000000 INFO @ Fri, 10 Dec 2021 16:46:54: 30000000 INFO @ Fri, 10 Dec 2021 16:46:55: 25000000 INFO @ Fri, 10 Dec 2021 16:47:02: 28000000 INFO @ Fri, 10 Dec 2021 16:47:04: 31000000 INFO @ Fri, 10 Dec 2021 16:47:06: 26000000 INFO @ Fri, 10 Dec 2021 16:47:13: 29000000 INFO @ Fri, 10 Dec 2021 16:47:15: 32000000 INFO @ Fri, 10 Dec 2021 16:47:16: 27000000 INFO @ Fri, 10 Dec 2021 16:47:24: 30000000 INFO @ Fri, 10 Dec 2021 16:47:26: 33000000 INFO @ Fri, 10 Dec 2021 16:47:27: 28000000 INFO @ Fri, 10 Dec 2021 16:47:34: 31000000 INFO @ Fri, 10 Dec 2021 16:47:37: 34000000 INFO @ Fri, 10 Dec 2021 16:47:38: 29000000 INFO @ Fri, 10 Dec 2021 16:47:45: 32000000 INFO @ Fri, 10 Dec 2021 16:47:47: 35000000 INFO @ Fri, 10 Dec 2021 16:47:49: 30000000 INFO @ Fri, 10 Dec 2021 16:47:55: 33000000 INFO @ Fri, 10 Dec 2021 16:47:58: 36000000 INFO @ Fri, 10 Dec 2021 16:47:59: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:48:06: 34000000 INFO @ Fri, 10 Dec 2021 16:48:09: 37000000 INFO @ Fri, 10 Dec 2021 16:48:10: 32000000 INFO @ Fri, 10 Dec 2021 16:48:17: 35000000 INFO @ Fri, 10 Dec 2021 16:48:19: 38000000 INFO @ Fri, 10 Dec 2021 16:48:20: 33000000 INFO @ Fri, 10 Dec 2021 16:48:27: 36000000 INFO @ Fri, 10 Dec 2021 16:48:30: 39000000 INFO @ Fri, 10 Dec 2021 16:48:31: 34000000 INFO @ Fri, 10 Dec 2021 16:48:38: 37000000 INFO @ Fri, 10 Dec 2021 16:48:41: 40000000 INFO @ Fri, 10 Dec 2021 16:48:42: 35000000 INFO @ Fri, 10 Dec 2021 16:48:48: 38000000 INFO @ Fri, 10 Dec 2021 16:48:52: 36000000 INFO @ Fri, 10 Dec 2021 16:48:53: 41000000 INFO @ Fri, 10 Dec 2021 16:48:59: 39000000 INFO @ Fri, 10 Dec 2021 16:49:03: 37000000 INFO @ Fri, 10 Dec 2021 16:49:03: 42000000 INFO @ Fri, 10 Dec 2021 16:49:10: 40000000 INFO @ Fri, 10 Dec 2021 16:49:13: 38000000 INFO @ Fri, 10 Dec 2021 16:49:14: 43000000 INFO @ Fri, 10 Dec 2021 16:49:21: 41000000 INFO @ Fri, 10 Dec 2021 16:49:24: 39000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:49:25: 44000000 INFO @ Fri, 10 Dec 2021 16:49:32: 42000000 INFO @ Fri, 10 Dec 2021 16:49:35: 40000000 INFO @ Fri, 10 Dec 2021 16:49:36: 45000000 INFO @ Fri, 10 Dec 2021 16:49:43: 43000000 INFO @ Fri, 10 Dec 2021 16:49:45: 41000000 INFO @ Fri, 10 Dec 2021 16:49:47: 46000000 INFO @ Fri, 10 Dec 2021 16:49:54: 44000000 INFO @ Fri, 10 Dec 2021 16:49:56: 42000000 INFO @ Fri, 10 Dec 2021 16:49:58: 47000000 INFO @ Fri, 10 Dec 2021 16:50:05: 45000000 INFO @ Fri, 10 Dec 2021 16:50:07: 43000000 INFO @ Fri, 10 Dec 2021 16:50:09: 48000000 INFO @ Fri, 10 Dec 2021 16:50:15: 46000000 INFO @ Fri, 10 Dec 2021 16:50:18: 44000000 INFO @ Fri, 10 Dec 2021 16:50:21: 49000000 INFO @ Fri, 10 Dec 2021 16:50:26: 47000000 INFO @ Fri, 10 Dec 2021 16:50:28: 45000000 INFO @ Fri, 10 Dec 2021 16:50:32: 50000000 INFO @ Fri, 10 Dec 2021 16:50:37: 48000000 INFO @ Fri, 10 Dec 2021 16:50:39: 46000000 INFO @ Fri, 10 Dec 2021 16:50:43: 51000000 INFO @ Fri, 10 Dec 2021 16:50:48: 49000000 INFO @ Fri, 10 Dec 2021 16:50:50: 47000000 INFO @ Fri, 10 Dec 2021 16:50:54: 52000000 INFO @ Fri, 10 Dec 2021 16:50:59: 50000000 INFO @ Fri, 10 Dec 2021 16:51:00: 48000000 INFO @ Fri, 10 Dec 2021 16:51:05: 53000000 INFO @ Fri, 10 Dec 2021 16:51:10: 51000000 INFO @ Fri, 10 Dec 2021 16:51:11: 49000000 INFO @ Fri, 10 Dec 2021 16:51:16: 54000000 INFO @ Fri, 10 Dec 2021 16:51:21: 52000000 INFO @ Fri, 10 Dec 2021 16:51:22: 50000000 INFO @ Fri, 10 Dec 2021 16:51:27: 55000000 INFO @ Fri, 10 Dec 2021 16:51:31: 53000000 INFO @ Fri, 10 Dec 2021 16:51:33: 51000000 INFO @ Fri, 10 Dec 2021 16:51:38: 56000000 INFO @ Fri, 10 Dec 2021 16:51:42: 54000000 INFO @ Fri, 10 Dec 2021 16:51:44: 52000000 INFO @ Fri, 10 Dec 2021 16:51:49: 57000000 INFO @ Fri, 10 Dec 2021 16:51:53: 55000000 INFO @ Fri, 10 Dec 2021 16:51:55: 53000000 INFO @ Fri, 10 Dec 2021 16:52:00: 58000000 INFO @ Fri, 10 Dec 2021 16:52:04: 56000000 INFO @ Fri, 10 Dec 2021 16:52:05: 54000000 INFO @ Fri, 10 Dec 2021 16:52:11: 59000000 INFO @ Fri, 10 Dec 2021 16:52:15: 57000000 INFO @ Fri, 10 Dec 2021 16:52:16: 55000000 INFO @ Fri, 10 Dec 2021 16:52:22: 60000000 INFO @ Fri, 10 Dec 2021 16:52:26: 58000000 INFO @ Fri, 10 Dec 2021 16:52:27: 56000000 INFO @ Fri, 10 Dec 2021 16:52:33: 61000000 INFO @ Fri, 10 Dec 2021 16:52:34: #1 tag size is determined as 119 bps INFO @ Fri, 10 Dec 2021 16:52:34: #1 tag size = 119 INFO @ Fri, 10 Dec 2021 16:52:34: #1 total tags in treatment: 27492727 INFO @ Fri, 10 Dec 2021 16:52:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:52:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:52:35: #1 tags after filtering in treatment: 20534068 INFO @ Fri, 10 Dec 2021 16:52:35: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 10 Dec 2021 16:52:35: #1 finished! INFO @ Fri, 10 Dec 2021 16:52:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:52:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:52:36: #2 number of paired peaks: 961 WARNING @ Fri, 10 Dec 2021 16:52:36: Fewer paired peaks (961) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 961 pairs to build model! INFO @ Fri, 10 Dec 2021 16:52:36: start model_add_line... INFO @ Fri, 10 Dec 2021 16:52:36: 59000000 INFO @ Fri, 10 Dec 2021 16:52:36: start X-correlation... INFO @ Fri, 10 Dec 2021 16:52:36: end of X-cor INFO @ Fri, 10 Dec 2021 16:52:36: #2 finished! INFO @ Fri, 10 Dec 2021 16:52:36: #2 predicted fragment length is 225 bps INFO @ Fri, 10 Dec 2021 16:52:36: #2 alternative fragment length(s) may be 2,225 bps INFO @ Fri, 10 Dec 2021 16:52:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.05_model.r WARNING @ Fri, 10 Dec 2021 16:52:36: #2 Since the d (225) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:52:36: #2 You may need to consider one of the other alternative d(s): 2,225 WARNING @ Fri, 10 Dec 2021 16:52:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:52:36: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:52:36: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:52:37: 57000000 INFO @ Fri, 10 Dec 2021 16:52:47: 60000000 INFO @ Fri, 10 Dec 2021 16:52:48: 58000000 INFO @ Fri, 10 Dec 2021 16:52:58: 61000000 INFO @ Fri, 10 Dec 2021 16:52:58: 59000000 INFO @ Fri, 10 Dec 2021 16:52:59: #1 tag size is determined as 119 bps INFO @ Fri, 10 Dec 2021 16:52:59: #1 tag size = 119 INFO @ Fri, 10 Dec 2021 16:52:59: #1 total tags in treatment: 27492727 INFO @ Fri, 10 Dec 2021 16:52:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:52:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:52:59: #1 tags after filtering in treatment: 20534068 INFO @ Fri, 10 Dec 2021 16:52:59: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 10 Dec 2021 16:52:59: #1 finished! INFO @ Fri, 10 Dec 2021 16:52:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:52:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:53:01: #2 number of paired peaks: 961 WARNING @ Fri, 10 Dec 2021 16:53:01: Fewer paired peaks (961) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 961 pairs to build model! INFO @ Fri, 10 Dec 2021 16:53:01: start model_add_line... INFO @ Fri, 10 Dec 2021 16:53:01: start X-correlation... INFO @ Fri, 10 Dec 2021 16:53:01: end of X-cor INFO @ Fri, 10 Dec 2021 16:53:01: #2 finished! INFO @ Fri, 10 Dec 2021 16:53:01: #2 predicted fragment length is 225 bps INFO @ Fri, 10 Dec 2021 16:53:01: #2 alternative fragment length(s) may be 2,225 bps INFO @ Fri, 10 Dec 2021 16:53:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.10_model.r WARNING @ Fri, 10 Dec 2021 16:53:01: #2 Since the d (225) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:53:01: #2 You may need to consider one of the other alternative d(s): 2,225 WARNING @ Fri, 10 Dec 2021 16:53:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:53:01: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:53:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:53:08: 60000000 INFO @ Fri, 10 Dec 2021 16:53:17: 61000000 INFO @ Fri, 10 Dec 2021 16:53:18: #1 tag size is determined as 119 bps INFO @ Fri, 10 Dec 2021 16:53:18: #1 tag size = 119 INFO @ Fri, 10 Dec 2021 16:53:18: #1 total tags in treatment: 27492727 INFO @ Fri, 10 Dec 2021 16:53:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:53:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:53:19: #1 tags after filtering in treatment: 20534068 INFO @ Fri, 10 Dec 2021 16:53:19: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 10 Dec 2021 16:53:19: #1 finished! INFO @ Fri, 10 Dec 2021 16:53:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:53:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:53:20: #2 number of paired peaks: 961 WARNING @ Fri, 10 Dec 2021 16:53:20: Fewer paired peaks (961) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 961 pairs to build model! INFO @ Fri, 10 Dec 2021 16:53:20: start model_add_line... INFO @ Fri, 10 Dec 2021 16:53:21: start X-correlation... INFO @ Fri, 10 Dec 2021 16:53:21: end of X-cor INFO @ Fri, 10 Dec 2021 16:53:21: #2 finished! INFO @ Fri, 10 Dec 2021 16:53:21: #2 predicted fragment length is 225 bps INFO @ Fri, 10 Dec 2021 16:53:21: #2 alternative fragment length(s) may be 2,225 bps INFO @ Fri, 10 Dec 2021 16:53:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.20_model.r WARNING @ Fri, 10 Dec 2021 16:53:21: #2 Since the d (225) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:53:21: #2 You may need to consider one of the other alternative d(s): 2,225 WARNING @ Fri, 10 Dec 2021 16:53:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:53:21: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:53:21: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:53:28: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:53:52: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:53:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.05_peaks.xls INFO @ Fri, 10 Dec 2021 16:53:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:53:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.05_summits.bed INFO @ Fri, 10 Dec 2021 16:53:54: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (12738 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:54:11: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:54:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.10_peaks.xls INFO @ Fri, 10 Dec 2021 16:54:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:54:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.10_summits.bed INFO @ Fri, 10 Dec 2021 16:54:18: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (9564 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:54:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.20_peaks.xls INFO @ Fri, 10 Dec 2021 16:54:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:54:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978817/ERX3978817.20_summits.bed INFO @ Fri, 10 Dec 2021 16:54:37: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (5892 records, 4 fields): 8 millis CompletedMACS2peakCalling