Job ID = 14167963 SRX = ERX3978808 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25000428 spots for ERR3975847/ERR3975847.sra Written 25000428 spots for ERR3975847/ERR3975847.sra Read 24694706 spots for ERR3975877/ERR3975877.sra Written 24694706 spots for ERR3975877/ERR3975877.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169003 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:03:28 49695134 reads; of these: 49695134 (100.00%) were paired; of these: 11331335 (22.80%) aligned concordantly 0 times 28193666 (56.73%) aligned concordantly exactly 1 time 10170133 (20.47%) aligned concordantly >1 times ---- 11331335 pairs aligned concordantly 0 times; of these: 5540672 (48.90%) aligned discordantly 1 time ---- 5790663 pairs aligned 0 times concordantly or discordantly; of these: 11581326 mates make up the pairs; of these: 6329292 (54.65%) aligned 0 times 1093324 (9.44%) aligned exactly 1 time 4158710 (35.91%) aligned >1 times 93.63% overall alignment rate Time searching: 02:03:29 Overall time: 02:03:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 5248140 / 43381823 = 0.1210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:36:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:36:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:36:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:36:55: 1000000 INFO @ Fri, 10 Dec 2021 16:37:01: 2000000 INFO @ Fri, 10 Dec 2021 16:37:07: 3000000 INFO @ Fri, 10 Dec 2021 16:37:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:37:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:37:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:37:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:37:19: 5000000 INFO @ Fri, 10 Dec 2021 16:37:26: 6000000 INFO @ Fri, 10 Dec 2021 16:37:27: 1000000 INFO @ Fri, 10 Dec 2021 16:37:32: 7000000 INFO @ Fri, 10 Dec 2021 16:37:35: 2000000 INFO @ Fri, 10 Dec 2021 16:37:39: 8000000 INFO @ Fri, 10 Dec 2021 16:37:43: 3000000 INFO @ Fri, 10 Dec 2021 16:37:46: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:37:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:37:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:37:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:37:50: 4000000 INFO @ Fri, 10 Dec 2021 16:37:53: 10000000 INFO @ Fri, 10 Dec 2021 16:37:57: 1000000 INFO @ Fri, 10 Dec 2021 16:37:58: 5000000 INFO @ Fri, 10 Dec 2021 16:38:00: 11000000 INFO @ Fri, 10 Dec 2021 16:38:04: 2000000 INFO @ Fri, 10 Dec 2021 16:38:06: 6000000 INFO @ Fri, 10 Dec 2021 16:38:07: 12000000 INFO @ Fri, 10 Dec 2021 16:38:12: 3000000 INFO @ Fri, 10 Dec 2021 16:38:13: 13000000 INFO @ Fri, 10 Dec 2021 16:38:14: 7000000 INFO @ Fri, 10 Dec 2021 16:38:20: 4000000 INFO @ Fri, 10 Dec 2021 16:38:20: 14000000 INFO @ Fri, 10 Dec 2021 16:38:22: 8000000 INFO @ Fri, 10 Dec 2021 16:38:27: 15000000 INFO @ Fri, 10 Dec 2021 16:38:27: 5000000 INFO @ Fri, 10 Dec 2021 16:38:30: 9000000 INFO @ Fri, 10 Dec 2021 16:38:34: 16000000 INFO @ Fri, 10 Dec 2021 16:38:35: 6000000 INFO @ Fri, 10 Dec 2021 16:38:37: 10000000 INFO @ Fri, 10 Dec 2021 16:38:41: 17000000 INFO @ Fri, 10 Dec 2021 16:38:43: 7000000 INFO @ Fri, 10 Dec 2021 16:38:45: 11000000 INFO @ Fri, 10 Dec 2021 16:38:48: 18000000 INFO @ Fri, 10 Dec 2021 16:38:50: 8000000 INFO @ Fri, 10 Dec 2021 16:38:53: 12000000 INFO @ Fri, 10 Dec 2021 16:38:55: 19000000 INFO @ Fri, 10 Dec 2021 16:38:58: 9000000 INFO @ Fri, 10 Dec 2021 16:39:00: 13000000 INFO @ Fri, 10 Dec 2021 16:39:02: 20000000 INFO @ Fri, 10 Dec 2021 16:39:06: 10000000 INFO @ Fri, 10 Dec 2021 16:39:08: 14000000 INFO @ Fri, 10 Dec 2021 16:39:09: 21000000 INFO @ Fri, 10 Dec 2021 16:39:14: 11000000 INFO @ Fri, 10 Dec 2021 16:39:16: 15000000 INFO @ Fri, 10 Dec 2021 16:39:16: 22000000 INFO @ Fri, 10 Dec 2021 16:39:21: 12000000 INFO @ Fri, 10 Dec 2021 16:39:23: 23000000 INFO @ Fri, 10 Dec 2021 16:39:23: 16000000 INFO @ Fri, 10 Dec 2021 16:39:29: 13000000 INFO @ Fri, 10 Dec 2021 16:39:30: 24000000 INFO @ Fri, 10 Dec 2021 16:39:31: 17000000 INFO @ Fri, 10 Dec 2021 16:39:37: 14000000 INFO @ Fri, 10 Dec 2021 16:39:37: 25000000 INFO @ Fri, 10 Dec 2021 16:39:39: 18000000 INFO @ Fri, 10 Dec 2021 16:39:44: 26000000 INFO @ Fri, 10 Dec 2021 16:39:44: 15000000 INFO @ Fri, 10 Dec 2021 16:39:46: 19000000 INFO @ Fri, 10 Dec 2021 16:39:50: 27000000 INFO @ Fri, 10 Dec 2021 16:39:52: 16000000 INFO @ Fri, 10 Dec 2021 16:39:54: 20000000 INFO @ Fri, 10 Dec 2021 16:39:57: 28000000 INFO @ Fri, 10 Dec 2021 16:40:00: 17000000 INFO @ Fri, 10 Dec 2021 16:40:02: 21000000 INFO @ Fri, 10 Dec 2021 16:40:04: 29000000 INFO @ Fri, 10 Dec 2021 16:40:07: 18000000 INFO @ Fri, 10 Dec 2021 16:40:10: 22000000 INFO @ Fri, 10 Dec 2021 16:40:11: 30000000 INFO @ Fri, 10 Dec 2021 16:40:15: 19000000 INFO @ Fri, 10 Dec 2021 16:40:17: 23000000 INFO @ Fri, 10 Dec 2021 16:40:17: 31000000 INFO @ Fri, 10 Dec 2021 16:40:23: 20000000 INFO @ Fri, 10 Dec 2021 16:40:24: 32000000 INFO @ Fri, 10 Dec 2021 16:40:25: 24000000 INFO @ Fri, 10 Dec 2021 16:40:31: 21000000 INFO @ Fri, 10 Dec 2021 16:40:31: 33000000 INFO @ Fri, 10 Dec 2021 16:40:32: 25000000 INFO @ Fri, 10 Dec 2021 16:40:38: 34000000 INFO @ Fri, 10 Dec 2021 16:40:38: 22000000 INFO @ Fri, 10 Dec 2021 16:40:40: 26000000 INFO @ Fri, 10 Dec 2021 16:40:44: 35000000 INFO @ Fri, 10 Dec 2021 16:40:46: 23000000 INFO @ Fri, 10 Dec 2021 16:40:47: 27000000 INFO @ Fri, 10 Dec 2021 16:40:51: 36000000 INFO @ Fri, 10 Dec 2021 16:40:54: 24000000 INFO @ Fri, 10 Dec 2021 16:40:55: 28000000 INFO @ Fri, 10 Dec 2021 16:40:58: 37000000 INFO @ Fri, 10 Dec 2021 16:41:01: 25000000 INFO @ Fri, 10 Dec 2021 16:41:02: 29000000 INFO @ Fri, 10 Dec 2021 16:41:04: 38000000 INFO @ Fri, 10 Dec 2021 16:41:09: 26000000 INFO @ Fri, 10 Dec 2021 16:41:10: 30000000 INFO @ Fri, 10 Dec 2021 16:41:11: 39000000 INFO @ Fri, 10 Dec 2021 16:41:16: 27000000 INFO @ Fri, 10 Dec 2021 16:41:17: 31000000 INFO @ Fri, 10 Dec 2021 16:41:18: 40000000 INFO @ Fri, 10 Dec 2021 16:41:24: 28000000 INFO @ Fri, 10 Dec 2021 16:41:24: 41000000 INFO @ Fri, 10 Dec 2021 16:41:25: 32000000 INFO @ Fri, 10 Dec 2021 16:41:31: 42000000 INFO @ Fri, 10 Dec 2021 16:41:31: 29000000 INFO @ Fri, 10 Dec 2021 16:41:32: 33000000 INFO @ Fri, 10 Dec 2021 16:41:38: 43000000 INFO @ Fri, 10 Dec 2021 16:41:39: 30000000 INFO @ Fri, 10 Dec 2021 16:41:40: 34000000 INFO @ Fri, 10 Dec 2021 16:41:44: 44000000 INFO @ Fri, 10 Dec 2021 16:41:46: 31000000 INFO @ Fri, 10 Dec 2021 16:41:48: 35000000 INFO @ Fri, 10 Dec 2021 16:41:51: 45000000 INFO @ Fri, 10 Dec 2021 16:41:54: 32000000 INFO @ Fri, 10 Dec 2021 16:41:55: 36000000 INFO @ Fri, 10 Dec 2021 16:41:58: 46000000 INFO @ Fri, 10 Dec 2021 16:42:02: 33000000 INFO @ Fri, 10 Dec 2021 16:42:03: 37000000 INFO @ Fri, 10 Dec 2021 16:42:05: 47000000 INFO @ Fri, 10 Dec 2021 16:42:10: 34000000 INFO @ Fri, 10 Dec 2021 16:42:11: 38000000 INFO @ Fri, 10 Dec 2021 16:42:11: 48000000 INFO @ Fri, 10 Dec 2021 16:42:17: 35000000 INFO @ Fri, 10 Dec 2021 16:42:18: 49000000 INFO @ Fri, 10 Dec 2021 16:42:19: 39000000 INFO @ Fri, 10 Dec 2021 16:42:25: 50000000 INFO @ Fri, 10 Dec 2021 16:42:25: 36000000 INFO @ Fri, 10 Dec 2021 16:42:27: 40000000 INFO @ Fri, 10 Dec 2021 16:42:32: 51000000 INFO @ Fri, 10 Dec 2021 16:42:33: 37000000 INFO @ Fri, 10 Dec 2021 16:42:35: 41000000 INFO @ Fri, 10 Dec 2021 16:42:38: 52000000 INFO @ Fri, 10 Dec 2021 16:42:40: 38000000 INFO @ Fri, 10 Dec 2021 16:42:43: 42000000 INFO @ Fri, 10 Dec 2021 16:42:45: 53000000 INFO @ Fri, 10 Dec 2021 16:42:48: 39000000 INFO @ Fri, 10 Dec 2021 16:42:50: 43000000 INFO @ Fri, 10 Dec 2021 16:42:51: 54000000 INFO @ Fri, 10 Dec 2021 16:42:55: 40000000 INFO @ Fri, 10 Dec 2021 16:42:58: 55000000 INFO @ Fri, 10 Dec 2021 16:42:58: 44000000 INFO @ Fri, 10 Dec 2021 16:43:03: 41000000 INFO @ Fri, 10 Dec 2021 16:43:05: 56000000 INFO @ Fri, 10 Dec 2021 16:43:06: 45000000 INFO @ Fri, 10 Dec 2021 16:43:10: 42000000 INFO @ Fri, 10 Dec 2021 16:43:11: 57000000 INFO @ Fri, 10 Dec 2021 16:43:13: 46000000 INFO @ Fri, 10 Dec 2021 16:43:18: 58000000 INFO @ Fri, 10 Dec 2021 16:43:18: 43000000 INFO @ Fri, 10 Dec 2021 16:43:21: 47000000 INFO @ Fri, 10 Dec 2021 16:43:24: 59000000 INFO @ Fri, 10 Dec 2021 16:43:26: 44000000 INFO @ Fri, 10 Dec 2021 16:43:29: 48000000 INFO @ Fri, 10 Dec 2021 16:43:30: 60000000 INFO @ Fri, 10 Dec 2021 16:43:33: 45000000 INFO @ Fri, 10 Dec 2021 16:43:36: 49000000 INFO @ Fri, 10 Dec 2021 16:43:37: 61000000 INFO @ Fri, 10 Dec 2021 16:43:41: 46000000 INFO @ Fri, 10 Dec 2021 16:43:43: 62000000 INFO @ Fri, 10 Dec 2021 16:43:44: 50000000 INFO @ Fri, 10 Dec 2021 16:43:49: 47000000 INFO @ Fri, 10 Dec 2021 16:43:50: 63000000 INFO @ Fri, 10 Dec 2021 16:43:52: 51000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:43:56: 64000000 INFO @ Fri, 10 Dec 2021 16:43:57: 48000000 INFO @ Fri, 10 Dec 2021 16:43:59: 52000000 INFO @ Fri, 10 Dec 2021 16:44:03: 65000000 INFO @ Fri, 10 Dec 2021 16:44:04: 49000000 INFO @ Fri, 10 Dec 2021 16:44:07: 53000000 INFO @ Fri, 10 Dec 2021 16:44:10: 66000000 INFO @ Fri, 10 Dec 2021 16:44:12: 50000000 INFO @ Fri, 10 Dec 2021 16:44:14: 54000000 INFO @ Fri, 10 Dec 2021 16:44:16: 67000000 INFO @ Fri, 10 Dec 2021 16:44:19: 51000000 INFO @ Fri, 10 Dec 2021 16:44:22: 55000000 INFO @ Fri, 10 Dec 2021 16:44:23: 68000000 INFO @ Fri, 10 Dec 2021 16:44:27: 52000000 INFO @ Fri, 10 Dec 2021 16:44:29: 69000000 INFO @ Fri, 10 Dec 2021 16:44:29: 56000000 INFO @ Fri, 10 Dec 2021 16:44:35: 53000000 INFO @ Fri, 10 Dec 2021 16:44:36: 70000000 INFO @ Fri, 10 Dec 2021 16:44:37: 57000000 INFO @ Fri, 10 Dec 2021 16:44:42: 54000000 INFO @ Fri, 10 Dec 2021 16:44:42: 71000000 INFO @ Fri, 10 Dec 2021 16:44:44: 58000000 INFO @ Fri, 10 Dec 2021 16:44:49: 72000000 INFO @ Fri, 10 Dec 2021 16:44:50: 55000000 INFO @ Fri, 10 Dec 2021 16:44:52: 59000000 INFO @ Fri, 10 Dec 2021 16:44:56: 73000000 INFO @ Fri, 10 Dec 2021 16:44:58: 56000000 INFO @ Fri, 10 Dec 2021 16:44:59: 60000000 INFO @ Fri, 10 Dec 2021 16:45:02: 74000000 INFO @ Fri, 10 Dec 2021 16:45:05: 57000000 INFO @ Fri, 10 Dec 2021 16:45:06: 61000000 INFO @ Fri, 10 Dec 2021 16:45:09: 75000000 INFO @ Fri, 10 Dec 2021 16:45:13: 58000000 INFO @ Fri, 10 Dec 2021 16:45:14: 62000000 INFO @ Fri, 10 Dec 2021 16:45:15: 76000000 INFO @ Fri, 10 Dec 2021 16:45:20: 59000000 INFO @ Fri, 10 Dec 2021 16:45:21: 63000000 INFO @ Fri, 10 Dec 2021 16:45:22: 77000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:45:27: 60000000 INFO @ Fri, 10 Dec 2021 16:45:29: 78000000 INFO @ Fri, 10 Dec 2021 16:45:29: 64000000 INFO @ Fri, 10 Dec 2021 16:45:35: 61000000 INFO @ Fri, 10 Dec 2021 16:45:35: 79000000 INFO @ Fri, 10 Dec 2021 16:45:36: 65000000 INFO @ Fri, 10 Dec 2021 16:45:42: 80000000 INFO @ Fri, 10 Dec 2021 16:45:43: 62000000 INFO @ Fri, 10 Dec 2021 16:45:44: 66000000 INFO @ Fri, 10 Dec 2021 16:45:48: 81000000 INFO @ Fri, 10 Dec 2021 16:45:50: 63000000 INFO @ Fri, 10 Dec 2021 16:45:52: 67000000 INFO @ Fri, 10 Dec 2021 16:45:55: 82000000 INFO @ Fri, 10 Dec 2021 16:45:58: 64000000 INFO @ Fri, 10 Dec 2021 16:45:59: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:45:59: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:45:59: #1 total tags in treatment: 33604015 INFO @ Fri, 10 Dec 2021 16:45:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:45:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:45:59: 68000000 INFO @ Fri, 10 Dec 2021 16:45:59: #1 tags after filtering in treatment: 25139231 INFO @ Fri, 10 Dec 2021 16:45:59: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 10 Dec 2021 16:45:59: #1 finished! INFO @ Fri, 10 Dec 2021 16:45:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:45:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:46:01: #2 number of paired peaks: 286 WARNING @ Fri, 10 Dec 2021 16:46:01: Fewer paired peaks (286) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 286 pairs to build model! INFO @ Fri, 10 Dec 2021 16:46:01: start model_add_line... INFO @ Fri, 10 Dec 2021 16:46:01: start X-correlation... INFO @ Fri, 10 Dec 2021 16:46:01: end of X-cor INFO @ Fri, 10 Dec 2021 16:46:01: #2 finished! INFO @ Fri, 10 Dec 2021 16:46:01: #2 predicted fragment length is 97 bps INFO @ Fri, 10 Dec 2021 16:46:01: #2 alternative fragment length(s) may be 4,97,102 bps INFO @ Fri, 10 Dec 2021 16:46:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.05_model.r WARNING @ Fri, 10 Dec 2021 16:46:01: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:46:01: #2 You may need to consider one of the other alternative d(s): 4,97,102 WARNING @ Fri, 10 Dec 2021 16:46:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:46:01: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:46:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:46:05: 65000000 INFO @ Fri, 10 Dec 2021 16:46:07: 69000000 INFO @ Fri, 10 Dec 2021 16:46:13: 66000000 INFO @ Fri, 10 Dec 2021 16:46:14: 70000000 INFO @ Fri, 10 Dec 2021 16:46:20: 67000000 INFO @ Fri, 10 Dec 2021 16:46:22: 71000000 INFO @ Fri, 10 Dec 2021 16:46:28: 68000000 INFO @ Fri, 10 Dec 2021 16:46:29: 72000000 INFO @ Fri, 10 Dec 2021 16:46:35: 69000000 INFO @ Fri, 10 Dec 2021 16:46:37: 73000000 INFO @ Fri, 10 Dec 2021 16:46:43: 70000000 INFO @ Fri, 10 Dec 2021 16:46:43: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:46:44: 74000000 INFO @ Fri, 10 Dec 2021 16:46:51: 71000000 INFO @ Fri, 10 Dec 2021 16:46:52: 75000000 INFO @ Fri, 10 Dec 2021 16:46:58: 72000000 INFO @ Fri, 10 Dec 2021 16:46:59: 76000000 INFO @ Fri, 10 Dec 2021 16:47:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.05_peaks.xls INFO @ Fri, 10 Dec 2021 16:47:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:47:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.05_summits.bed INFO @ Fri, 10 Dec 2021 16:47:06: 73000000 INFO @ Fri, 10 Dec 2021 16:47:06: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (12849 records, 4 fields): 199 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:47:07: 77000000 INFO @ Fri, 10 Dec 2021 16:47:13: 74000000 INFO @ Fri, 10 Dec 2021 16:47:14: 78000000 INFO @ Fri, 10 Dec 2021 16:47:21: 75000000 INFO @ Fri, 10 Dec 2021 16:47:22: 79000000 INFO @ Fri, 10 Dec 2021 16:47:28: 76000000 INFO @ Fri, 10 Dec 2021 16:47:29: 80000000 INFO @ Fri, 10 Dec 2021 16:47:36: 77000000 INFO @ Fri, 10 Dec 2021 16:47:37: 81000000 INFO @ Fri, 10 Dec 2021 16:47:43: 78000000 INFO @ Fri, 10 Dec 2021 16:47:44: 82000000 INFO @ Fri, 10 Dec 2021 16:47:49: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:47:49: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:47:49: #1 total tags in treatment: 33604015 INFO @ Fri, 10 Dec 2021 16:47:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:47:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:47:49: #1 tags after filtering in treatment: 25139231 INFO @ Fri, 10 Dec 2021 16:47:49: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 10 Dec 2021 16:47:49: #1 finished! INFO @ Fri, 10 Dec 2021 16:47:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:47:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:47:51: #2 number of paired peaks: 286 WARNING @ Fri, 10 Dec 2021 16:47:51: Fewer paired peaks (286) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 286 pairs to build model! INFO @ Fri, 10 Dec 2021 16:47:51: start model_add_line... INFO @ Fri, 10 Dec 2021 16:47:51: 79000000 INFO @ Fri, 10 Dec 2021 16:47:51: start X-correlation... INFO @ Fri, 10 Dec 2021 16:47:51: end of X-cor INFO @ Fri, 10 Dec 2021 16:47:51: #2 finished! INFO @ Fri, 10 Dec 2021 16:47:51: #2 predicted fragment length is 97 bps INFO @ Fri, 10 Dec 2021 16:47:51: #2 alternative fragment length(s) may be 4,97,102 bps INFO @ Fri, 10 Dec 2021 16:47:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.10_model.r WARNING @ Fri, 10 Dec 2021 16:47:51: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:47:51: #2 You may need to consider one of the other alternative d(s): 4,97,102 WARNING @ Fri, 10 Dec 2021 16:47:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:47:51: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:47:51: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:47:58: 80000000 INFO @ Fri, 10 Dec 2021 16:48:06: 81000000 INFO @ Fri, 10 Dec 2021 16:48:13: 82000000 INFO @ Fri, 10 Dec 2021 16:48:17: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:48:17: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:48:17: #1 total tags in treatment: 33604015 INFO @ Fri, 10 Dec 2021 16:48:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:48:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:48:18: #1 tags after filtering in treatment: 25139231 INFO @ Fri, 10 Dec 2021 16:48:18: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 10 Dec 2021 16:48:18: #1 finished! INFO @ Fri, 10 Dec 2021 16:48:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:48:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:48:20: #2 number of paired peaks: 286 WARNING @ Fri, 10 Dec 2021 16:48:20: Fewer paired peaks (286) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 286 pairs to build model! INFO @ Fri, 10 Dec 2021 16:48:20: start model_add_line... INFO @ Fri, 10 Dec 2021 16:48:20: start X-correlation... INFO @ Fri, 10 Dec 2021 16:48:20: end of X-cor INFO @ Fri, 10 Dec 2021 16:48:20: #2 finished! INFO @ Fri, 10 Dec 2021 16:48:20: #2 predicted fragment length is 97 bps INFO @ Fri, 10 Dec 2021 16:48:20: #2 alternative fragment length(s) may be 4,97,102 bps INFO @ Fri, 10 Dec 2021 16:48:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.20_model.r WARNING @ Fri, 10 Dec 2021 16:48:20: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:48:20: #2 You may need to consider one of the other alternative d(s): 4,97,102 WARNING @ Fri, 10 Dec 2021 16:48:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:48:20: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:48:20: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:48:33: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:48:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.10_peaks.xls INFO @ Fri, 10 Dec 2021 16:48:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:48:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.10_summits.bed INFO @ Fri, 10 Dec 2021 16:48:55: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (8830 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:49:03: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:49:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.20_peaks.xls INFO @ Fri, 10 Dec 2021 16:49:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:49:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978808/ERX3978808.20_summits.bed INFO @ Fri, 10 Dec 2021 16:49:24: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (3859 records, 4 fields): 17 millis CompletedMACS2peakCalling