Job ID = 14169723 SRX = ERX3978787 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 24041036 spots for ERR3975816/ERR3975816.sra Written 24041036 spots for ERR3975816/ERR3975816.sra Read 26431966 spots for ERR3975891/ERR3975891.sra Written 26431966 spots for ERR3975891/ERR3975891.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170690 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:21:04 50473002 reads; of these: 50473002 (100.00%) were paired; of these: 16903661 (33.49%) aligned concordantly 0 times 19910045 (39.45%) aligned concordantly exactly 1 time 13659296 (27.06%) aligned concordantly >1 times ---- 16903661 pairs aligned concordantly 0 times; of these: 6049005 (35.79%) aligned discordantly 1 time ---- 10854656 pairs aligned 0 times concordantly or discordantly; of these: 21709312 mates make up the pairs; of these: 12317672 (56.74%) aligned 0 times 957741 (4.41%) aligned exactly 1 time 8433899 (38.85%) aligned >1 times 87.80% overall alignment rate Time searching: 02:21:05 Overall time: 02:21:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 9249810 / 38983534 = 0.2373 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:21:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:21:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:21:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:21:10: 1000000 INFO @ Sat, 11 Dec 2021 06:21:19: 2000000 INFO @ Sat, 11 Dec 2021 06:21:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:21:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:21:31: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:21:31: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:21:37: 4000000 INFO @ Sat, 11 Dec 2021 06:21:38: 1000000 INFO @ Sat, 11 Dec 2021 06:21:44: 2000000 INFO @ Sat, 11 Dec 2021 06:21:45: 5000000 INFO @ Sat, 11 Dec 2021 06:21:50: 3000000 INFO @ Sat, 11 Dec 2021 06:21:53: 6000000 INFO @ Sat, 11 Dec 2021 06:21:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:22:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:22:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:22:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:22:01: 7000000 INFO @ Sat, 11 Dec 2021 06:22:03: 5000000 INFO @ Sat, 11 Dec 2021 06:22:08: 1000000 INFO @ Sat, 11 Dec 2021 06:22:10: 6000000 INFO @ Sat, 11 Dec 2021 06:22:10: 8000000 INFO @ Sat, 11 Dec 2021 06:22:15: 2000000 INFO @ Sat, 11 Dec 2021 06:22:16: 7000000 INFO @ Sat, 11 Dec 2021 06:22:18: 9000000 INFO @ Sat, 11 Dec 2021 06:22:22: 3000000 INFO @ Sat, 11 Dec 2021 06:22:23: 8000000 INFO @ Sat, 11 Dec 2021 06:22:27: 10000000 INFO @ Sat, 11 Dec 2021 06:22:29: 4000000 INFO @ Sat, 11 Dec 2021 06:22:30: 9000000 INFO @ Sat, 11 Dec 2021 06:22:35: 11000000 INFO @ Sat, 11 Dec 2021 06:22:36: 5000000 INFO @ Sat, 11 Dec 2021 06:22:37: 10000000 INFO @ Sat, 11 Dec 2021 06:22:43: 6000000 INFO @ Sat, 11 Dec 2021 06:22:44: 11000000 INFO @ Sat, 11 Dec 2021 06:22:45: 12000000 INFO @ Sat, 11 Dec 2021 06:22:50: 7000000 INFO @ Sat, 11 Dec 2021 06:22:50: 12000000 INFO @ Sat, 11 Dec 2021 06:22:54: 13000000 INFO @ Sat, 11 Dec 2021 06:22:57: 8000000 INFO @ Sat, 11 Dec 2021 06:22:57: 13000000 INFO @ Sat, 11 Dec 2021 06:23:02: 14000000 INFO @ Sat, 11 Dec 2021 06:23:03: 9000000 INFO @ Sat, 11 Dec 2021 06:23:04: 14000000 INFO @ Sat, 11 Dec 2021 06:23:10: 10000000 INFO @ Sat, 11 Dec 2021 06:23:11: 15000000 INFO @ Sat, 11 Dec 2021 06:23:11: 15000000 INFO @ Sat, 11 Dec 2021 06:23:17: 11000000 INFO @ Sat, 11 Dec 2021 06:23:19: 16000000 INFO @ Sat, 11 Dec 2021 06:23:19: 16000000 INFO @ Sat, 11 Dec 2021 06:23:24: 12000000 INFO @ Sat, 11 Dec 2021 06:23:28: 17000000 INFO @ Sat, 11 Dec 2021 06:23:28: 17000000 INFO @ Sat, 11 Dec 2021 06:23:31: 13000000 INFO @ Sat, 11 Dec 2021 06:23:36: 18000000 INFO @ Sat, 11 Dec 2021 06:23:36: 18000000 INFO @ Sat, 11 Dec 2021 06:23:37: 14000000 INFO @ Sat, 11 Dec 2021 06:23:44: 15000000 INFO @ Sat, 11 Dec 2021 06:23:44: 19000000 INFO @ Sat, 11 Dec 2021 06:23:45: 19000000 INFO @ Sat, 11 Dec 2021 06:23:51: 16000000 INFO @ Sat, 11 Dec 2021 06:23:51: 20000000 INFO @ Sat, 11 Dec 2021 06:23:52: 20000000 INFO @ Sat, 11 Dec 2021 06:23:58: 17000000 INFO @ Sat, 11 Dec 2021 06:23:59: 21000000 INFO @ Sat, 11 Dec 2021 06:24:00: 21000000 INFO @ Sat, 11 Dec 2021 06:24:04: 18000000 INFO @ Sat, 11 Dec 2021 06:24:06: 22000000 INFO @ Sat, 11 Dec 2021 06:24:08: 22000000 INFO @ Sat, 11 Dec 2021 06:24:11: 19000000 INFO @ Sat, 11 Dec 2021 06:24:14: 23000000 INFO @ Sat, 11 Dec 2021 06:24:15: 23000000 INFO @ Sat, 11 Dec 2021 06:24:18: 20000000 INFO @ Sat, 11 Dec 2021 06:24:22: 24000000 INFO @ Sat, 11 Dec 2021 06:24:23: 24000000 INFO @ Sat, 11 Dec 2021 06:24:24: 21000000 INFO @ Sat, 11 Dec 2021 06:24:30: 25000000 INFO @ Sat, 11 Dec 2021 06:24:31: 25000000 INFO @ Sat, 11 Dec 2021 06:24:31: 22000000 INFO @ Sat, 11 Dec 2021 06:24:37: 26000000 INFO @ Sat, 11 Dec 2021 06:24:38: 23000000 INFO @ Sat, 11 Dec 2021 06:24:38: 26000000 INFO @ Sat, 11 Dec 2021 06:24:44: 27000000 INFO @ Sat, 11 Dec 2021 06:24:45: 24000000 INFO @ Sat, 11 Dec 2021 06:24:46: 27000000 INFO @ Sat, 11 Dec 2021 06:24:51: 28000000 INFO @ Sat, 11 Dec 2021 06:24:52: 25000000 INFO @ Sat, 11 Dec 2021 06:24:54: 28000000 INFO @ Sat, 11 Dec 2021 06:24:58: 29000000 INFO @ Sat, 11 Dec 2021 06:24:58: 26000000 INFO @ Sat, 11 Dec 2021 06:25:02: 29000000 INFO @ Sat, 11 Dec 2021 06:25:05: 27000000 INFO @ Sat, 11 Dec 2021 06:25:06: 30000000 INFO @ Sat, 11 Dec 2021 06:25:10: 30000000 INFO @ Sat, 11 Dec 2021 06:25:12: 28000000 INFO @ Sat, 11 Dec 2021 06:25:12: 31000000 INFO @ Sat, 11 Dec 2021 06:25:17: 31000000 INFO @ Sat, 11 Dec 2021 06:25:19: 29000000 INFO @ Sat, 11 Dec 2021 06:25:19: 32000000 INFO @ Sat, 11 Dec 2021 06:25:25: 32000000 INFO @ Sat, 11 Dec 2021 06:25:26: 30000000 INFO @ Sat, 11 Dec 2021 06:25:27: 33000000 INFO @ Sat, 11 Dec 2021 06:25:32: 31000000 INFO @ Sat, 11 Dec 2021 06:25:33: 33000000 INFO @ Sat, 11 Dec 2021 06:25:35: 34000000 INFO @ Sat, 11 Dec 2021 06:25:39: 32000000 INFO @ Sat, 11 Dec 2021 06:25:40: 34000000 INFO @ Sat, 11 Dec 2021 06:25:41: 35000000 INFO @ Sat, 11 Dec 2021 06:25:46: 33000000 INFO @ Sat, 11 Dec 2021 06:25:48: 35000000 INFO @ Sat, 11 Dec 2021 06:25:48: 36000000 INFO @ Sat, 11 Dec 2021 06:25:52: 34000000 INFO @ Sat, 11 Dec 2021 06:25:55: 37000000 INFO @ Sat, 11 Dec 2021 06:25:55: 36000000 INFO @ Sat, 11 Dec 2021 06:25:59: 35000000 INFO @ Sat, 11 Dec 2021 06:26:01: 38000000 INFO @ Sat, 11 Dec 2021 06:26:03: 37000000 INFO @ Sat, 11 Dec 2021 06:26:06: 36000000 INFO @ Sat, 11 Dec 2021 06:26:08: 39000000 INFO @ Sat, 11 Dec 2021 06:26:10: 38000000 INFO @ Sat, 11 Dec 2021 06:26:12: 37000000 INFO @ Sat, 11 Dec 2021 06:26:15: 40000000 INFO @ Sat, 11 Dec 2021 06:26:17: 39000000 INFO @ Sat, 11 Dec 2021 06:26:19: 38000000 INFO @ Sat, 11 Dec 2021 06:26:21: 41000000 INFO @ Sat, 11 Dec 2021 06:26:25: 40000000 INFO @ Sat, 11 Dec 2021 06:26:26: 39000000 INFO @ Sat, 11 Dec 2021 06:26:28: 42000000 INFO @ Sat, 11 Dec 2021 06:26:32: 41000000 INFO @ Sat, 11 Dec 2021 06:26:33: 40000000 INFO @ Sat, 11 Dec 2021 06:26:35: 43000000 INFO @ Sat, 11 Dec 2021 06:26:39: 41000000 INFO @ Sat, 11 Dec 2021 06:26:40: 42000000 INFO @ Sat, 11 Dec 2021 06:26:42: 44000000 INFO @ Sat, 11 Dec 2021 06:26:46: 42000000 INFO @ Sat, 11 Dec 2021 06:26:48: 43000000 INFO @ Sat, 11 Dec 2021 06:26:49: 45000000 INFO @ Sat, 11 Dec 2021 06:26:54: 43000000 INFO @ Sat, 11 Dec 2021 06:26:55: 44000000 INFO @ Sat, 11 Dec 2021 06:26:55: 46000000 INFO @ Sat, 11 Dec 2021 06:27:01: 44000000 INFO @ Sat, 11 Dec 2021 06:27:02: 47000000 INFO @ Sat, 11 Dec 2021 06:27:02: 45000000 INFO @ Sat, 11 Dec 2021 06:27:07: 45000000 INFO @ Sat, 11 Dec 2021 06:27:08: 48000000 INFO @ Sat, 11 Dec 2021 06:27:09: 46000000 INFO @ Sat, 11 Dec 2021 06:27:14: 46000000 INFO @ Sat, 11 Dec 2021 06:27:15: 49000000 INFO @ Sat, 11 Dec 2021 06:27:16: 47000000 INFO @ Sat, 11 Dec 2021 06:27:21: 47000000 INFO @ Sat, 11 Dec 2021 06:27:22: 50000000 INFO @ Sat, 11 Dec 2021 06:27:24: 48000000 INFO @ Sat, 11 Dec 2021 06:27:27: 48000000 INFO @ Sat, 11 Dec 2021 06:27:28: 51000000 INFO @ Sat, 11 Dec 2021 06:27:31: 49000000 INFO @ Sat, 11 Dec 2021 06:27:34: 49000000 INFO @ Sat, 11 Dec 2021 06:27:35: 52000000 INFO @ Sat, 11 Dec 2021 06:27:38: 50000000 INFO @ Sat, 11 Dec 2021 06:27:41: 50000000 INFO @ Sat, 11 Dec 2021 06:27:41: 53000000 INFO @ Sat, 11 Dec 2021 06:27:45: 51000000 INFO @ Sat, 11 Dec 2021 06:27:48: 51000000 INFO @ Sat, 11 Dec 2021 06:27:48: 54000000 INFO @ Sat, 11 Dec 2021 06:27:52: 52000000 INFO @ Sat, 11 Dec 2021 06:27:54: 52000000 INFO @ Sat, 11 Dec 2021 06:27:55: 55000000 INFO @ Sat, 11 Dec 2021 06:27:59: 53000000 INFO @ Sat, 11 Dec 2021 06:28:01: 53000000 INFO @ Sat, 11 Dec 2021 06:28:01: 56000000 INFO @ Sat, 11 Dec 2021 06:28:06: 54000000 INFO @ Sat, 11 Dec 2021 06:28:07: 54000000 INFO @ Sat, 11 Dec 2021 06:28:08: 57000000 INFO @ Sat, 11 Dec 2021 06:28:13: 55000000 INFO @ Sat, 11 Dec 2021 06:28:14: 55000000 INFO @ Sat, 11 Dec 2021 06:28:15: 58000000 INFO @ Sat, 11 Dec 2021 06:28:20: 56000000 INFO @ Sat, 11 Dec 2021 06:28:21: 56000000 INFO @ Sat, 11 Dec 2021 06:28:22: 59000000 INFO @ Sat, 11 Dec 2021 06:28:28: 57000000 INFO @ Sat, 11 Dec 2021 06:28:28: 57000000 INFO @ Sat, 11 Dec 2021 06:28:29: 60000000 INFO @ Sat, 11 Dec 2021 06:28:36: 58000000 INFO @ Sat, 11 Dec 2021 06:28:36: 58000000 INFO @ Sat, 11 Dec 2021 06:28:36: 61000000 INFO @ Sat, 11 Dec 2021 06:28:43: 62000000 INFO @ Sat, 11 Dec 2021 06:28:43: 59000000 INFO @ Sat, 11 Dec 2021 06:28:44: 59000000 INFO @ Sat, 11 Dec 2021 06:28:50: 63000000 INFO @ Sat, 11 Dec 2021 06:28:50: 60000000 INFO @ Sat, 11 Dec 2021 06:28:51: 60000000 INFO @ Sat, 11 Dec 2021 06:28:57: 64000000 INFO @ Sat, 11 Dec 2021 06:28:58: 61000000 INFO @ Sat, 11 Dec 2021 06:28:58: 61000000 INFO @ Sat, 11 Dec 2021 06:29:04: 65000000 INFO @ Sat, 11 Dec 2021 06:29:05: 62000000 INFO @ Sat, 11 Dec 2021 06:29:06: 62000000 INFO @ Sat, 11 Dec 2021 06:29:10: 66000000 INFO @ Sat, 11 Dec 2021 06:29:11: 63000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:29:13: 63000000 INFO @ Sat, 11 Dec 2021 06:29:17: 67000000 INFO @ Sat, 11 Dec 2021 06:29:18: 64000000 INFO @ Sat, 11 Dec 2021 06:29:21: 64000000 INFO @ Sat, 11 Dec 2021 06:29:24: 68000000 INFO @ Sat, 11 Dec 2021 06:29:25: 65000000 INFO @ Sat, 11 Dec 2021 06:29:28: 65000000 INFO @ Sat, 11 Dec 2021 06:29:31: 69000000 INFO @ Sat, 11 Dec 2021 06:29:32: 66000000 INFO @ Sat, 11 Dec 2021 06:29:35: 66000000 INFO @ Sat, 11 Dec 2021 06:29:38: 70000000 INFO @ Sat, 11 Dec 2021 06:29:39: 67000000 INFO @ Sat, 11 Dec 2021 06:29:39: #1 tag size is determined as 118 bps INFO @ Sat, 11 Dec 2021 06:29:39: #1 tag size = 118 INFO @ Sat, 11 Dec 2021 06:29:39: #1 total tags in treatment: 24632064 INFO @ Sat, 11 Dec 2021 06:29:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:29:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:29:40: #1 tags after filtering in treatment: 18756643 INFO @ Sat, 11 Dec 2021 06:29:40: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 11 Dec 2021 06:29:40: #1 finished! INFO @ Sat, 11 Dec 2021 06:29:40: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:29:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:29:41: #2 number of paired peaks: 870 WARNING @ Sat, 11 Dec 2021 06:29:41: Fewer paired peaks (870) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 870 pairs to build model! INFO @ Sat, 11 Dec 2021 06:29:41: start model_add_line... INFO @ Sat, 11 Dec 2021 06:29:41: start X-correlation... INFO @ Sat, 11 Dec 2021 06:29:42: end of X-cor INFO @ Sat, 11 Dec 2021 06:29:42: #2 finished! INFO @ Sat, 11 Dec 2021 06:29:42: #2 predicted fragment length is 122 bps INFO @ Sat, 11 Dec 2021 06:29:42: #2 alternative fragment length(s) may be 122 bps INFO @ Sat, 11 Dec 2021 06:29:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.10_model.r WARNING @ Sat, 11 Dec 2021 06:29:42: #2 Since the d (122) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:29:42: #2 You may need to consider one of the other alternative d(s): 122 WARNING @ Sat, 11 Dec 2021 06:29:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:29:42: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:29:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:29:43: 67000000 INFO @ Sat, 11 Dec 2021 06:29:45: 68000000 INFO @ Sat, 11 Dec 2021 06:29:50: 68000000 INFO @ Sat, 11 Dec 2021 06:29:52: 69000000 INFO @ Sat, 11 Dec 2021 06:29:58: 69000000 INFO @ Sat, 11 Dec 2021 06:29:58: 70000000 INFO @ Sat, 11 Dec 2021 06:29:59: #1 tag size is determined as 118 bps INFO @ Sat, 11 Dec 2021 06:29:59: #1 tag size = 118 INFO @ Sat, 11 Dec 2021 06:29:59: #1 total tags in treatment: 24632064 INFO @ Sat, 11 Dec 2021 06:29:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:29:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:30:00: #1 tags after filtering in treatment: 18756643 INFO @ Sat, 11 Dec 2021 06:30:00: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 11 Dec 2021 06:30:00: #1 finished! INFO @ Sat, 11 Dec 2021 06:30:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:30:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:30:01: #2 number of paired peaks: 870 WARNING @ Sat, 11 Dec 2021 06:30:01: Fewer paired peaks (870) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 870 pairs to build model! INFO @ Sat, 11 Dec 2021 06:30:01: start model_add_line... INFO @ Sat, 11 Dec 2021 06:30:01: start X-correlation... INFO @ Sat, 11 Dec 2021 06:30:01: end of X-cor INFO @ Sat, 11 Dec 2021 06:30:01: #2 finished! INFO @ Sat, 11 Dec 2021 06:30:01: #2 predicted fragment length is 122 bps INFO @ Sat, 11 Dec 2021 06:30:01: #2 alternative fragment length(s) may be 122 bps INFO @ Sat, 11 Dec 2021 06:30:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.20_model.r WARNING @ Sat, 11 Dec 2021 06:30:01: #2 Since the d (122) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:30:01: #2 You may need to consider one of the other alternative d(s): 122 WARNING @ Sat, 11 Dec 2021 06:30:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:30:01: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:30:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:30:05: 70000000 INFO @ Sat, 11 Dec 2021 06:30:06: #1 tag size is determined as 118 bps INFO @ Sat, 11 Dec 2021 06:30:06: #1 tag size = 118 INFO @ Sat, 11 Dec 2021 06:30:06: #1 total tags in treatment: 24632064 INFO @ Sat, 11 Dec 2021 06:30:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:30:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:30:07: #1 tags after filtering in treatment: 18756643 INFO @ Sat, 11 Dec 2021 06:30:07: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 11 Dec 2021 06:30:07: #1 finished! INFO @ Sat, 11 Dec 2021 06:30:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:30:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:30:08: #2 number of paired peaks: 870 WARNING @ Sat, 11 Dec 2021 06:30:08: Fewer paired peaks (870) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 870 pairs to build model! INFO @ Sat, 11 Dec 2021 06:30:08: start model_add_line... INFO @ Sat, 11 Dec 2021 06:30:08: start X-correlation... INFO @ Sat, 11 Dec 2021 06:30:08: end of X-cor INFO @ Sat, 11 Dec 2021 06:30:08: #2 finished! INFO @ Sat, 11 Dec 2021 06:30:08: #2 predicted fragment length is 122 bps INFO @ Sat, 11 Dec 2021 06:30:08: #2 alternative fragment length(s) may be 122 bps INFO @ Sat, 11 Dec 2021 06:30:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.05_model.r WARNING @ Sat, 11 Dec 2021 06:30:09: #2 Since the d (122) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:30:09: #2 You may need to consider one of the other alternative d(s): 122 WARNING @ Sat, 11 Dec 2021 06:30:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:30:09: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:30:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:30:18: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:30:38: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:30:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.10_peaks.xls INFO @ Sat, 11 Dec 2021 06:30:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:30:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.10_summits.bed INFO @ Sat, 11 Dec 2021 06:30:38: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (9940 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:30:46: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:30:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.20_peaks.xls INFO @ Sat, 11 Dec 2021 06:30:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:30:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.20_summits.bed INFO @ Sat, 11 Dec 2021 06:30:58: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (5059 records, 4 fields): 8 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:31:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.05_peaks.xls INFO @ Sat, 11 Dec 2021 06:31:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:31:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978787/ERX3978787.05_summits.bed INFO @ Sat, 11 Dec 2021 06:31:07: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (14387 records, 4 fields): 18 millis CompletedMACS2peakCalling