Job ID = 6527438 SRX = ERX2075302 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-29T12:27:07 prefetch.2.10.7: 1) Downloading 'ERR2015720'... 2020-06-29T12:27:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:32:53 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:32:53 prefetch.2.10.7: 1) 'ERR2015720' was downloaded successfully Read 27526859 spots for ERR2015720/ERR2015720.sra Written 27526859 spots for ERR2015720/ERR2015720.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:04:55 27526859 reads; of these: 27526859 (100.00%) were paired; of these: 2022622 (7.35%) aligned concordantly 0 times 18505450 (67.23%) aligned concordantly exactly 1 time 6998787 (25.43%) aligned concordantly >1 times ---- 2022622 pairs aligned concordantly 0 times; of these: 370431 (18.31%) aligned discordantly 1 time ---- 1652191 pairs aligned 0 times concordantly or discordantly; of these: 3304382 mates make up the pairs; of these: 2064266 (62.47%) aligned 0 times 699261 (21.16%) aligned exactly 1 time 540855 (16.37%) aligned >1 times 96.25% overall alignment rate Time searching: 01:04:56 Overall time: 01:04:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1260973 / 25652902 = 0.0492 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:07:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:07:48: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:07:48: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:07:53: 1000000 INFO @ Mon, 29 Jun 2020 23:07:59: 2000000 INFO @ Mon, 29 Jun 2020 23:08:05: 3000000 INFO @ Mon, 29 Jun 2020 23:08:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:08:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:08:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:08:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:08:18: 5000000 INFO @ Mon, 29 Jun 2020 23:08:26: 1000000 INFO @ Mon, 29 Jun 2020 23:08:26: 6000000 INFO @ Mon, 29 Jun 2020 23:08:34: 7000000 INFO @ Mon, 29 Jun 2020 23:08:34: 2000000 INFO @ Mon, 29 Jun 2020 23:08:41: 8000000 INFO @ Mon, 29 Jun 2020 23:08:42: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:08:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:08:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:08:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:08:49: 9000000 INFO @ Mon, 29 Jun 2020 23:08:50: 4000000 INFO @ Mon, 29 Jun 2020 23:08:56: 1000000 INFO @ Mon, 29 Jun 2020 23:08:58: 10000000 INFO @ Mon, 29 Jun 2020 23:08:59: 5000000 INFO @ Mon, 29 Jun 2020 23:09:04: 2000000 INFO @ Mon, 29 Jun 2020 23:09:06: 11000000 INFO @ Mon, 29 Jun 2020 23:09:07: 6000000 INFO @ Mon, 29 Jun 2020 23:09:12: 3000000 INFO @ Mon, 29 Jun 2020 23:09:14: 12000000 INFO @ Mon, 29 Jun 2020 23:09:15: 7000000 INFO @ Mon, 29 Jun 2020 23:09:20: 4000000 INFO @ Mon, 29 Jun 2020 23:09:22: 13000000 INFO @ Mon, 29 Jun 2020 23:09:24: 8000000 INFO @ Mon, 29 Jun 2020 23:09:28: 5000000 INFO @ Mon, 29 Jun 2020 23:09:30: 14000000 INFO @ Mon, 29 Jun 2020 23:09:32: 9000000 INFO @ Mon, 29 Jun 2020 23:09:37: 6000000 INFO @ Mon, 29 Jun 2020 23:09:39: 15000000 INFO @ Mon, 29 Jun 2020 23:09:40: 10000000 INFO @ Mon, 29 Jun 2020 23:09:45: 7000000 INFO @ Mon, 29 Jun 2020 23:09:47: 16000000 INFO @ Mon, 29 Jun 2020 23:09:48: 11000000 INFO @ Mon, 29 Jun 2020 23:09:53: 8000000 INFO @ Mon, 29 Jun 2020 23:09:55: 17000000 INFO @ Mon, 29 Jun 2020 23:09:57: 12000000 INFO @ Mon, 29 Jun 2020 23:10:01: 9000000 INFO @ Mon, 29 Jun 2020 23:10:03: 18000000 INFO @ Mon, 29 Jun 2020 23:10:05: 13000000 INFO @ Mon, 29 Jun 2020 23:10:09: 10000000 INFO @ Mon, 29 Jun 2020 23:10:11: 19000000 INFO @ Mon, 29 Jun 2020 23:10:13: 14000000 INFO @ Mon, 29 Jun 2020 23:10:17: 11000000 INFO @ Mon, 29 Jun 2020 23:10:19: 20000000 INFO @ Mon, 29 Jun 2020 23:10:21: 15000000 INFO @ Mon, 29 Jun 2020 23:10:26: 12000000 INFO @ Mon, 29 Jun 2020 23:10:28: 21000000 INFO @ Mon, 29 Jun 2020 23:10:30: 16000000 INFO @ Mon, 29 Jun 2020 23:10:34: 13000000 INFO @ Mon, 29 Jun 2020 23:10:36: 22000000 INFO @ Mon, 29 Jun 2020 23:10:38: 17000000 INFO @ Mon, 29 Jun 2020 23:10:42: 14000000 INFO @ Mon, 29 Jun 2020 23:10:44: 23000000 INFO @ Mon, 29 Jun 2020 23:10:46: 18000000 INFO @ Mon, 29 Jun 2020 23:10:50: 15000000 INFO @ Mon, 29 Jun 2020 23:10:52: 24000000 INFO @ Mon, 29 Jun 2020 23:10:54: 19000000 INFO @ Mon, 29 Jun 2020 23:10:58: 16000000 INFO @ Mon, 29 Jun 2020 23:11:00: 25000000 INFO @ Mon, 29 Jun 2020 23:11:03: 20000000 INFO @ Mon, 29 Jun 2020 23:11:07: 17000000 INFO @ Mon, 29 Jun 2020 23:11:09: 26000000 INFO @ Mon, 29 Jun 2020 23:11:11: 21000000 INFO @ Mon, 29 Jun 2020 23:11:15: 18000000 INFO @ Mon, 29 Jun 2020 23:11:17: 27000000 INFO @ Mon, 29 Jun 2020 23:11:19: 22000000 INFO @ Mon, 29 Jun 2020 23:11:23: 19000000 INFO @ Mon, 29 Jun 2020 23:11:25: 28000000 INFO @ Mon, 29 Jun 2020 23:11:27: 23000000 INFO @ Mon, 29 Jun 2020 23:11:31: 20000000 INFO @ Mon, 29 Jun 2020 23:11:33: 29000000 INFO @ Mon, 29 Jun 2020 23:11:36: 24000000 INFO @ Mon, 29 Jun 2020 23:11:39: 21000000 INFO @ Mon, 29 Jun 2020 23:11:41: 30000000 INFO @ Mon, 29 Jun 2020 23:11:44: 25000000 INFO @ Mon, 29 Jun 2020 23:11:48: 22000000 INFO @ Mon, 29 Jun 2020 23:11:49: 31000000 INFO @ Mon, 29 Jun 2020 23:11:52: 26000000 INFO @ Mon, 29 Jun 2020 23:11:56: 23000000 INFO @ Mon, 29 Jun 2020 23:11:58: 32000000 INFO @ Mon, 29 Jun 2020 23:12:00: 27000000 INFO @ Mon, 29 Jun 2020 23:12:04: 24000000 INFO @ Mon, 29 Jun 2020 23:12:06: 33000000 INFO @ Mon, 29 Jun 2020 23:12:08: 28000000 INFO @ Mon, 29 Jun 2020 23:12:12: 25000000 INFO @ Mon, 29 Jun 2020 23:12:14: 34000000 INFO @ Mon, 29 Jun 2020 23:12:16: 29000000 INFO @ Mon, 29 Jun 2020 23:12:21: 26000000 INFO @ Mon, 29 Jun 2020 23:12:22: 35000000 INFO @ Mon, 29 Jun 2020 23:12:25: 30000000 INFO @ Mon, 29 Jun 2020 23:12:29: 27000000 INFO @ Mon, 29 Jun 2020 23:12:30: 36000000 INFO @ Mon, 29 Jun 2020 23:12:33: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:12:37: 28000000 INFO @ Mon, 29 Jun 2020 23:12:39: 37000000 INFO @ Mon, 29 Jun 2020 23:12:41: 32000000 INFO @ Mon, 29 Jun 2020 23:12:45: 29000000 INFO @ Mon, 29 Jun 2020 23:12:47: 38000000 INFO @ Mon, 29 Jun 2020 23:12:49: 33000000 INFO @ Mon, 29 Jun 2020 23:12:53: 30000000 INFO @ Mon, 29 Jun 2020 23:12:55: 39000000 INFO @ Mon, 29 Jun 2020 23:12:57: 34000000 INFO @ Mon, 29 Jun 2020 23:13:02: 31000000 INFO @ Mon, 29 Jun 2020 23:13:04: 40000000 INFO @ Mon, 29 Jun 2020 23:13:05: 35000000 INFO @ Mon, 29 Jun 2020 23:13:10: 32000000 INFO @ Mon, 29 Jun 2020 23:13:12: 41000000 INFO @ Mon, 29 Jun 2020 23:13:14: 36000000 INFO @ Mon, 29 Jun 2020 23:13:18: 33000000 INFO @ Mon, 29 Jun 2020 23:13:21: 42000000 INFO @ Mon, 29 Jun 2020 23:13:22: 37000000 INFO @ Mon, 29 Jun 2020 23:13:26: 34000000 INFO @ Mon, 29 Jun 2020 23:13:29: 43000000 INFO @ Mon, 29 Jun 2020 23:13:30: 38000000 INFO @ Mon, 29 Jun 2020 23:13:34: 35000000 INFO @ Mon, 29 Jun 2020 23:13:37: 44000000 INFO @ Mon, 29 Jun 2020 23:13:38: 39000000 INFO @ Mon, 29 Jun 2020 23:13:42: 36000000 INFO @ Mon, 29 Jun 2020 23:13:45: 45000000 INFO @ Mon, 29 Jun 2020 23:13:46: 40000000 INFO @ Mon, 29 Jun 2020 23:13:50: 37000000 INFO @ Mon, 29 Jun 2020 23:13:53: 46000000 INFO @ Mon, 29 Jun 2020 23:13:55: 41000000 INFO @ Mon, 29 Jun 2020 23:13:58: 38000000 INFO @ Mon, 29 Jun 2020 23:14:01: 47000000 INFO @ Mon, 29 Jun 2020 23:14:03: 42000000 INFO @ Mon, 29 Jun 2020 23:14:06: 39000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:14:09: 48000000 INFO @ Mon, 29 Jun 2020 23:14:11: 43000000 INFO @ Mon, 29 Jun 2020 23:14:15: 40000000 INFO @ Mon, 29 Jun 2020 23:14:17: 49000000 INFO @ Mon, 29 Jun 2020 23:14:20: 44000000 INFO @ Mon, 29 Jun 2020 23:14:23: 41000000 INFO @ Mon, 29 Jun 2020 23:14:25: 50000000 INFO @ Mon, 29 Jun 2020 23:14:28: 45000000 INFO @ Mon, 29 Jun 2020 23:14:29: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 23:14:29: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 23:14:29: #1 total tags in treatment: 24249398 INFO @ Mon, 29 Jun 2020 23:14:29: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:14:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:14:29: #1 tags after filtering in treatment: 22295072 INFO @ Mon, 29 Jun 2020 23:14:29: #1 Redundant rate of treatment: 0.08 INFO @ Mon, 29 Jun 2020 23:14:29: #1 finished! INFO @ Mon, 29 Jun 2020 23:14:29: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:14:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:14:31: 42000000 INFO @ Mon, 29 Jun 2020 23:14:31: #2 number of paired peaks: 97 WARNING @ Mon, 29 Jun 2020 23:14:31: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:14:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:14:36: 46000000 INFO @ Mon, 29 Jun 2020 23:14:39: 43000000 INFO @ Mon, 29 Jun 2020 23:14:44: 47000000 INFO @ Mon, 29 Jun 2020 23:14:46: 44000000 INFO @ Mon, 29 Jun 2020 23:14:53: 48000000 INFO @ Mon, 29 Jun 2020 23:14:54: 45000000 INFO @ Mon, 29 Jun 2020 23:15:01: 49000000 INFO @ Mon, 29 Jun 2020 23:15:02: 46000000 INFO @ Mon, 29 Jun 2020 23:15:09: 50000000 INFO @ Mon, 29 Jun 2020 23:15:09: 47000000 INFO @ Mon, 29 Jun 2020 23:15:13: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 23:15:13: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 23:15:13: #1 total tags in treatment: 24249398 INFO @ Mon, 29 Jun 2020 23:15:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:15:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:15:13: #1 tags after filtering in treatment: 22295072 INFO @ Mon, 29 Jun 2020 23:15:13: #1 Redundant rate of treatment: 0.08 INFO @ Mon, 29 Jun 2020 23:15:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:15:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:15:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:15:15: #2 number of paired peaks: 97 WARNING @ Mon, 29 Jun 2020 23:15:15: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:15:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:15:16: 48000000 INFO @ Mon, 29 Jun 2020 23:15:22: 49000000 INFO @ Mon, 29 Jun 2020 23:15:28: 50000000 INFO @ Mon, 29 Jun 2020 23:15:31: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 23:15:31: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 23:15:31: #1 total tags in treatment: 24249398 INFO @ Mon, 29 Jun 2020 23:15:31: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:15:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:15:31: #1 tags after filtering in treatment: 22295072 INFO @ Mon, 29 Jun 2020 23:15:31: #1 Redundant rate of treatment: 0.08 INFO @ Mon, 29 Jun 2020 23:15:31: #1 finished! INFO @ Mon, 29 Jun 2020 23:15:31: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:15:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:15:33: #2 number of paired peaks: 97 WARNING @ Mon, 29 Jun 2020 23:15:33: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:15:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX2075302/ERX2075302.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling