Job ID = 6527431 SRX = ERX1818278 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-29T12:35:26 prefetch.2.10.7: 1) Downloading 'ERR1749081'... 2020-06-29T12:35:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:43:48 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:43:48 prefetch.2.10.7: 1) 'ERR1749081' was downloaded successfully Read 32036737 spots for ERR1749081/ERR1749081.sra Written 32036737 spots for ERR1749081/ERR1749081.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:11:36 32036737 reads; of these: 32036737 (100.00%) were paired; of these: 4639841 (14.48%) aligned concordantly 0 times 20197715 (63.05%) aligned concordantly exactly 1 time 7199181 (22.47%) aligned concordantly >1 times ---- 4639841 pairs aligned concordantly 0 times; of these: 191818 (4.13%) aligned discordantly 1 time ---- 4448023 pairs aligned 0 times concordantly or discordantly; of these: 8896046 mates make up the pairs; of these: 7016804 (78.88%) aligned 0 times 938380 (10.55%) aligned exactly 1 time 940862 (10.58%) aligned >1 times 89.05% overall alignment rate Time searching: 01:11:36 Overall time: 01:11:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3091906 / 27489665 = 0.1125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:35:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:35:57: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:35:57: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:36:03: 1000000 INFO @ Mon, 29 Jun 2020 23:36:10: 2000000 INFO @ Mon, 29 Jun 2020 23:36:16: 3000000 INFO @ Mon, 29 Jun 2020 23:36:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:36:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:36:27: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:36:27: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:36:28: 5000000 INFO @ Mon, 29 Jun 2020 23:36:32: 1000000 INFO @ Mon, 29 Jun 2020 23:36:35: 6000000 INFO @ Mon, 29 Jun 2020 23:36:38: 2000000 INFO @ Mon, 29 Jun 2020 23:36:41: 7000000 INFO @ Mon, 29 Jun 2020 23:36:43: 3000000 INFO @ Mon, 29 Jun 2020 23:36:47: 8000000 INFO @ Mon, 29 Jun 2020 23:36:49: 4000000 INFO @ Mon, 29 Jun 2020 23:36:53: 9000000 INFO @ Mon, 29 Jun 2020 23:36:54: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:36:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:36:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:36:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:37:00: 6000000 INFO @ Mon, 29 Jun 2020 23:37:00: 10000000 INFO @ Mon, 29 Jun 2020 23:37:02: 1000000 INFO @ Mon, 29 Jun 2020 23:37:05: 7000000 INFO @ Mon, 29 Jun 2020 23:37:06: 11000000 INFO @ Mon, 29 Jun 2020 23:37:07: 2000000 INFO @ Mon, 29 Jun 2020 23:37:10: 8000000 INFO @ Mon, 29 Jun 2020 23:37:12: 12000000 INFO @ Mon, 29 Jun 2020 23:37:13: 3000000 INFO @ Mon, 29 Jun 2020 23:37:16: 9000000 INFO @ Mon, 29 Jun 2020 23:37:18: 4000000 INFO @ Mon, 29 Jun 2020 23:37:18: 13000000 INFO @ Mon, 29 Jun 2020 23:37:21: 10000000 INFO @ Mon, 29 Jun 2020 23:37:24: 5000000 INFO @ Mon, 29 Jun 2020 23:37:25: 14000000 INFO @ Mon, 29 Jun 2020 23:37:27: 11000000 INFO @ Mon, 29 Jun 2020 23:37:29: 6000000 INFO @ Mon, 29 Jun 2020 23:37:31: 15000000 INFO @ Mon, 29 Jun 2020 23:37:32: 12000000 INFO @ Mon, 29 Jun 2020 23:37:35: 7000000 INFO @ Mon, 29 Jun 2020 23:37:38: 16000000 INFO @ Mon, 29 Jun 2020 23:37:38: 13000000 INFO @ Mon, 29 Jun 2020 23:37:40: 8000000 INFO @ Mon, 29 Jun 2020 23:37:43: 14000000 INFO @ Mon, 29 Jun 2020 23:37:44: 17000000 INFO @ Mon, 29 Jun 2020 23:37:46: 9000000 INFO @ Mon, 29 Jun 2020 23:37:49: 15000000 INFO @ Mon, 29 Jun 2020 23:37:51: 18000000 INFO @ Mon, 29 Jun 2020 23:37:51: 10000000 INFO @ Mon, 29 Jun 2020 23:37:55: 16000000 INFO @ Mon, 29 Jun 2020 23:37:57: 11000000 INFO @ Mon, 29 Jun 2020 23:37:57: 19000000 INFO @ Mon, 29 Jun 2020 23:38:00: 17000000 INFO @ Mon, 29 Jun 2020 23:38:03: 12000000 INFO @ Mon, 29 Jun 2020 23:38:04: 20000000 INFO @ Mon, 29 Jun 2020 23:38:06: 18000000 INFO @ Mon, 29 Jun 2020 23:38:08: 13000000 INFO @ Mon, 29 Jun 2020 23:38:10: 21000000 INFO @ Mon, 29 Jun 2020 23:38:12: 19000000 INFO @ Mon, 29 Jun 2020 23:38:14: 14000000 INFO @ Mon, 29 Jun 2020 23:38:16: 22000000 INFO @ Mon, 29 Jun 2020 23:38:17: 20000000 INFO @ Mon, 29 Jun 2020 23:38:20: 15000000 INFO @ Mon, 29 Jun 2020 23:38:22: 23000000 INFO @ Mon, 29 Jun 2020 23:38:23: 21000000 INFO @ Mon, 29 Jun 2020 23:38:26: 16000000 INFO @ Mon, 29 Jun 2020 23:38:28: 24000000 INFO @ Mon, 29 Jun 2020 23:38:29: 22000000 INFO @ Mon, 29 Jun 2020 23:38:31: 17000000 INFO @ Mon, 29 Jun 2020 23:38:34: 25000000 INFO @ Mon, 29 Jun 2020 23:38:35: 23000000 INFO @ Mon, 29 Jun 2020 23:38:37: 18000000 INFO @ Mon, 29 Jun 2020 23:38:40: 24000000 INFO @ Mon, 29 Jun 2020 23:38:40: 26000000 INFO @ Mon, 29 Jun 2020 23:38:42: 19000000 INFO @ Mon, 29 Jun 2020 23:38:46: 25000000 INFO @ Mon, 29 Jun 2020 23:38:47: 27000000 INFO @ Mon, 29 Jun 2020 23:38:48: 20000000 INFO @ Mon, 29 Jun 2020 23:38:52: 26000000 INFO @ Mon, 29 Jun 2020 23:38:53: 28000000 INFO @ Mon, 29 Jun 2020 23:38:54: 21000000 INFO @ Mon, 29 Jun 2020 23:38:57: 27000000 INFO @ Mon, 29 Jun 2020 23:38:59: 29000000 INFO @ Mon, 29 Jun 2020 23:39:00: 22000000 INFO @ Mon, 29 Jun 2020 23:39:03: 28000000 INFO @ Mon, 29 Jun 2020 23:39:05: 30000000 INFO @ Mon, 29 Jun 2020 23:39:05: 23000000 INFO @ Mon, 29 Jun 2020 23:39:09: 29000000 INFO @ Mon, 29 Jun 2020 23:39:11: 31000000 INFO @ Mon, 29 Jun 2020 23:39:11: 24000000 INFO @ Mon, 29 Jun 2020 23:39:14: 30000000 INFO @ Mon, 29 Jun 2020 23:39:16: 25000000 INFO @ Mon, 29 Jun 2020 23:39:16: 32000000 INFO @ Mon, 29 Jun 2020 23:39:20: 31000000 INFO @ Mon, 29 Jun 2020 23:39:22: 26000000 INFO @ Mon, 29 Jun 2020 23:39:22: 33000000 INFO @ Mon, 29 Jun 2020 23:39:25: 32000000 INFO @ Mon, 29 Jun 2020 23:39:27: 27000000 INFO @ Mon, 29 Jun 2020 23:39:29: 34000000 INFO @ Mon, 29 Jun 2020 23:39:31: 33000000 INFO @ Mon, 29 Jun 2020 23:39:33: 28000000 INFO @ Mon, 29 Jun 2020 23:39:35: 35000000 INFO @ Mon, 29 Jun 2020 23:39:36: 34000000 INFO @ Mon, 29 Jun 2020 23:39:39: 29000000 INFO @ Mon, 29 Jun 2020 23:39:41: 36000000 INFO @ Mon, 29 Jun 2020 23:39:41: 35000000 INFO @ Mon, 29 Jun 2020 23:39:44: 30000000 INFO @ Mon, 29 Jun 2020 23:39:47: 37000000 INFO @ Mon, 29 Jun 2020 23:39:47: 36000000 INFO @ Mon, 29 Jun 2020 23:39:50: 31000000 INFO @ Mon, 29 Jun 2020 23:39:52: 37000000 INFO @ Mon, 29 Jun 2020 23:39:53: 38000000 INFO @ Mon, 29 Jun 2020 23:39:56: 32000000 INFO @ Mon, 29 Jun 2020 23:39:58: 38000000 INFO @ Mon, 29 Jun 2020 23:39:59: 39000000 INFO @ Mon, 29 Jun 2020 23:40:01: 33000000 INFO @ Mon, 29 Jun 2020 23:40:03: 39000000 INFO @ Mon, 29 Jun 2020 23:40:06: 40000000 INFO @ Mon, 29 Jun 2020 23:40:07: 34000000 INFO @ Mon, 29 Jun 2020 23:40:09: 40000000 INFO @ Mon, 29 Jun 2020 23:40:12: 41000000 INFO @ Mon, 29 Jun 2020 23:40:12: 35000000 INFO @ Mon, 29 Jun 2020 23:40:14: 41000000 INFO @ Mon, 29 Jun 2020 23:40:17: 36000000 INFO @ Mon, 29 Jun 2020 23:40:18: 42000000 INFO @ Mon, 29 Jun 2020 23:40:20: 42000000 INFO @ Mon, 29 Jun 2020 23:40:23: 37000000 INFO @ Mon, 29 Jun 2020 23:40:24: 43000000 INFO @ Mon, 29 Jun 2020 23:40:25: 43000000 INFO @ Mon, 29 Jun 2020 23:40:28: 38000000 INFO @ Mon, 29 Jun 2020 23:40:30: 44000000 INFO @ Mon, 29 Jun 2020 23:40:31: 44000000 INFO @ Mon, 29 Jun 2020 23:40:34: 39000000 INFO @ Mon, 29 Jun 2020 23:40:36: 45000000 INFO @ Mon, 29 Jun 2020 23:40:37: 45000000 INFO @ Mon, 29 Jun 2020 23:40:39: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:40:42: 46000000 INFO @ Mon, 29 Jun 2020 23:40:43: 46000000 INFO @ Mon, 29 Jun 2020 23:40:45: 41000000 INFO @ Mon, 29 Jun 2020 23:40:48: 47000000 INFO @ Mon, 29 Jun 2020 23:40:49: 47000000 INFO @ Mon, 29 Jun 2020 23:40:50: 42000000 INFO @ Mon, 29 Jun 2020 23:40:54: 48000000 INFO @ Mon, 29 Jun 2020 23:40:55: 48000000 INFO @ Mon, 29 Jun 2020 23:40:55: 43000000 INFO @ Mon, 29 Jun 2020 23:41:00: 49000000 INFO @ Mon, 29 Jun 2020 23:41:01: 44000000 INFO @ Mon, 29 Jun 2020 23:41:01: 49000000 INFO @ Mon, 29 Jun 2020 23:41:06: 45000000 INFO @ Mon, 29 Jun 2020 23:41:07: 50000000 INFO @ Mon, 29 Jun 2020 23:41:07: 50000000 INFO @ Mon, 29 Jun 2020 23:41:11: 46000000 INFO @ Mon, 29 Jun 2020 23:41:12: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 23:41:12: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 23:41:12: #1 total tags in treatment: 24311237 INFO @ Mon, 29 Jun 2020 23:41:12: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:13: #1 tags after filtering in treatment: 22593500 INFO @ Mon, 29 Jun 2020 23:41:13: #1 Redundant rate of treatment: 0.07 INFO @ Mon, 29 Jun 2020 23:41:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:13: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 23:41:13: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 23:41:13: #1 total tags in treatment: 24311237 INFO @ Mon, 29 Jun 2020 23:41:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:13: #1 tags after filtering in treatment: 22593500 INFO @ Mon, 29 Jun 2020 23:41:13: #1 Redundant rate of treatment: 0.07 INFO @ Mon, 29 Jun 2020 23:41:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:14: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:41:14: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:41:15: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:41:15: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:41:16: 47000000 INFO @ Mon, 29 Jun 2020 23:41:21: 48000000 INFO @ Mon, 29 Jun 2020 23:41:26: 49000000 INFO @ Mon, 29 Jun 2020 23:41:31: 50000000 INFO @ Mon, 29 Jun 2020 23:41:36: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 23:41:36: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 23:41:36: #1 total tags in treatment: 24311237 INFO @ Mon, 29 Jun 2020 23:41:36: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:36: #1 tags after filtering in treatment: 22593500 INFO @ Mon, 29 Jun 2020 23:41:36: #1 Redundant rate of treatment: 0.07 INFO @ Mon, 29 Jun 2020 23:41:36: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:36: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:38: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:41:38: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818278/ERX1818278.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。