Job ID = 5720357 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T15:00:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 15,637,546 reads read : 31,275,092 reads written : 31,275,092 2020-04-15T15:36:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 15,535,983 reads read : 31,071,966 reads written : 31,071,966 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:12:25 31173529 reads; of these: 31173529 (100.00%) were paired; of these: 2752065 (8.83%) aligned concordantly 0 times 21400238 (68.65%) aligned concordantly exactly 1 time 7021226 (22.52%) aligned concordantly >1 times ---- 2752065 pairs aligned concordantly 0 times; of these: 830706 (30.18%) aligned discordantly 1 time ---- 1921359 pairs aligned 0 times concordantly or discordantly; of these: 3842718 mates make up the pairs; of these: 2050907 (53.37%) aligned 0 times 1046995 (27.25%) aligned exactly 1 time 744816 (19.38%) aligned >1 times 96.71% overall alignment rate Time searching: 01:12:25 Overall time: 01:12:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3241580 / 29141773 = 0.1112 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 02:29:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 02:29:37: #1 read tag files... INFO @ Thu, 16 Apr 2020 02:29:37: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 02:29:42: 1000000 INFO @ Thu, 16 Apr 2020 02:29:48: 2000000 INFO @ Thu, 16 Apr 2020 02:29:53: 3000000 INFO @ Thu, 16 Apr 2020 02:29:58: 4000000 INFO @ Thu, 16 Apr 2020 02:30:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 02:30:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 02:30:08: #1 read tag files... INFO @ Thu, 16 Apr 2020 02:30:08: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 02:30:08: 6000000 INFO @ Thu, 16 Apr 2020 02:30:13: 7000000 INFO @ Thu, 16 Apr 2020 02:30:13: 1000000 INFO @ Thu, 16 Apr 2020 02:30:19: 8000000 INFO @ Thu, 16 Apr 2020 02:30:19: 2000000 INFO @ Thu, 16 Apr 2020 02:30:24: 9000000 INFO @ Thu, 16 Apr 2020 02:30:24: 3000000 INFO @ Thu, 16 Apr 2020 02:30:29: 10000000 INFO @ Thu, 16 Apr 2020 02:30:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 02:30:34: 11000000 INFO @ Thu, 16 Apr 2020 02:30:35: 5000000 INFO @ Thu, 16 Apr 2020 02:30:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 02:30:38: #1 read tag files... INFO @ Thu, 16 Apr 2020 02:30:38: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 02:30:40: 12000000 INFO @ Thu, 16 Apr 2020 02:30:40: 6000000 INFO @ Thu, 16 Apr 2020 02:30:44: 1000000 INFO @ Thu, 16 Apr 2020 02:30:45: 13000000 INFO @ Thu, 16 Apr 2020 02:30:45: 7000000 INFO @ Thu, 16 Apr 2020 02:30:50: 2000000 INFO @ Thu, 16 Apr 2020 02:30:50: 14000000 INFO @ Thu, 16 Apr 2020 02:30:51: 8000000 INFO @ Thu, 16 Apr 2020 02:30:56: 15000000 INFO @ Thu, 16 Apr 2020 02:30:56: 9000000 INFO @ Thu, 16 Apr 2020 02:30:56: 3000000 INFO @ Thu, 16 Apr 2020 02:31:01: 16000000 INFO @ Thu, 16 Apr 2020 02:31:01: 10000000 INFO @ Thu, 16 Apr 2020 02:31:02: 4000000 INFO @ Thu, 16 Apr 2020 02:31:06: 17000000 INFO @ Thu, 16 Apr 2020 02:31:06: 11000000 INFO @ Thu, 16 Apr 2020 02:31:09: 5000000 INFO @ Thu, 16 Apr 2020 02:31:11: 18000000 INFO @ Thu, 16 Apr 2020 02:31:12: 12000000 INFO @ Thu, 16 Apr 2020 02:31:15: 6000000 INFO @ Thu, 16 Apr 2020 02:31:17: 19000000 INFO @ Thu, 16 Apr 2020 02:31:17: 13000000 INFO @ Thu, 16 Apr 2020 02:31:21: 7000000 INFO @ Thu, 16 Apr 2020 02:31:22: 20000000 INFO @ Thu, 16 Apr 2020 02:31:23: 14000000 INFO @ Thu, 16 Apr 2020 02:31:28: 8000000 INFO @ Thu, 16 Apr 2020 02:31:28: 15000000 INFO @ Thu, 16 Apr 2020 02:31:29: 21000000 INFO @ Thu, 16 Apr 2020 02:31:33: 16000000 INFO @ Thu, 16 Apr 2020 02:31:34: 9000000 INFO @ Thu, 16 Apr 2020 02:31:34: 22000000 INFO @ Thu, 16 Apr 2020 02:31:39: 17000000 INFO @ Thu, 16 Apr 2020 02:31:39: 23000000 INFO @ Thu, 16 Apr 2020 02:31:40: 10000000 INFO @ Thu, 16 Apr 2020 02:31:44: 18000000 INFO @ Thu, 16 Apr 2020 02:31:45: 24000000 INFO @ Thu, 16 Apr 2020 02:31:46: 11000000 INFO @ Thu, 16 Apr 2020 02:31:49: 19000000 INFO @ Thu, 16 Apr 2020 02:31:50: 25000000 INFO @ Thu, 16 Apr 2020 02:31:55: 12000000 INFO @ Thu, 16 Apr 2020 02:31:55: 20000000 INFO @ Thu, 16 Apr 2020 02:31:55: 26000000 INFO @ Thu, 16 Apr 2020 02:32:00: 27000000 INFO @ Thu, 16 Apr 2020 02:32:01: 21000000 INFO @ Thu, 16 Apr 2020 02:32:02: 13000000 INFO @ Thu, 16 Apr 2020 02:32:06: 28000000 INFO @ Thu, 16 Apr 2020 02:32:07: 22000000 INFO @ Thu, 16 Apr 2020 02:32:11: 29000000 INFO @ Thu, 16 Apr 2020 02:32:11: 14000000 INFO @ Thu, 16 Apr 2020 02:32:12: 23000000 INFO @ Thu, 16 Apr 2020 02:32:16: 30000000 INFO @ Thu, 16 Apr 2020 02:32:17: 24000000 INFO @ Thu, 16 Apr 2020 02:32:18: 15000000 INFO @ Thu, 16 Apr 2020 02:32:21: 31000000 INFO @ Thu, 16 Apr 2020 02:32:23: 25000000 INFO @ Thu, 16 Apr 2020 02:32:24: 16000000 INFO @ Thu, 16 Apr 2020 02:32:27: 32000000 INFO @ Thu, 16 Apr 2020 02:32:28: 26000000 INFO @ Thu, 16 Apr 2020 02:32:31: 17000000 INFO @ Thu, 16 Apr 2020 02:32:32: 33000000 INFO @ Thu, 16 Apr 2020 02:32:34: 27000000 INFO @ Thu, 16 Apr 2020 02:32:37: 34000000 INFO @ Thu, 16 Apr 2020 02:32:37: 18000000 INFO @ Thu, 16 Apr 2020 02:32:39: 28000000 INFO @ Thu, 16 Apr 2020 02:32:42: 35000000 INFO @ Thu, 16 Apr 2020 02:32:43: 19000000 INFO @ Thu, 16 Apr 2020 02:32:44: 29000000 INFO @ Thu, 16 Apr 2020 02:32:48: 36000000 INFO @ Thu, 16 Apr 2020 02:32:49: 20000000 INFO @ Thu, 16 Apr 2020 02:32:49: 30000000 INFO @ Thu, 16 Apr 2020 02:32:53: 37000000 INFO @ Thu, 16 Apr 2020 02:32:54: 21000000 INFO @ Thu, 16 Apr 2020 02:32:55: 31000000 INFO @ Thu, 16 Apr 2020 02:32:58: 38000000 INFO @ Thu, 16 Apr 2020 02:33:00: 32000000 INFO @ Thu, 16 Apr 2020 02:33:00: 22000000 INFO @ Thu, 16 Apr 2020 02:33:03: 39000000 INFO @ Thu, 16 Apr 2020 02:33:05: 33000000 INFO @ Thu, 16 Apr 2020 02:33:07: 23000000 INFO @ Thu, 16 Apr 2020 02:33:09: 40000000 INFO @ Thu, 16 Apr 2020 02:33:10: 34000000 INFO @ Thu, 16 Apr 2020 02:33:13: 24000000 INFO @ Thu, 16 Apr 2020 02:33:14: 41000000 INFO @ Thu, 16 Apr 2020 02:33:16: 35000000 INFO @ Thu, 16 Apr 2020 02:33:19: 42000000 INFO @ Thu, 16 Apr 2020 02:33:19: 25000000 INFO @ Thu, 16 Apr 2020 02:33:21: 36000000 INFO @ Thu, 16 Apr 2020 02:33:24: 43000000 INFO @ Thu, 16 Apr 2020 02:33:26: 26000000 INFO @ Thu, 16 Apr 2020 02:33:26: 37000000 INFO @ Thu, 16 Apr 2020 02:33:30: 44000000 INFO @ Thu, 16 Apr 2020 02:33:32: 27000000 INFO @ Thu, 16 Apr 2020 02:33:32: 38000000 INFO @ Thu, 16 Apr 2020 02:33:35: 45000000 INFO @ Thu, 16 Apr 2020 02:33:37: 39000000 INFO @ Thu, 16 Apr 2020 02:33:38: 28000000 INFO @ Thu, 16 Apr 2020 02:33:40: 46000000 INFO @ Thu, 16 Apr 2020 02:33:42: 40000000 INFO @ Thu, 16 Apr 2020 02:33:44: 29000000 INFO @ Thu, 16 Apr 2020 02:33:45: 47000000 INFO @ Thu, 16 Apr 2020 02:33:47: 41000000 INFO @ Thu, 16 Apr 2020 02:33:50: 30000000 INFO @ Thu, 16 Apr 2020 02:33:51: 48000000 INFO @ Thu, 16 Apr 2020 02:33:53: 42000000 INFO @ Thu, 16 Apr 2020 02:33:56: 31000000 INFO @ Thu, 16 Apr 2020 02:33:56: 49000000 INFO @ Thu, 16 Apr 2020 02:33:58: 43000000 INFO @ Thu, 16 Apr 2020 02:34:01: 50000000 INFO @ Thu, 16 Apr 2020 02:34:02: 32000000 INFO @ Thu, 16 Apr 2020 02:34:03: 44000000 INFO @ Thu, 16 Apr 2020 02:34:07: 51000000 INFO @ Thu, 16 Apr 2020 02:34:08: 45000000 INFO @ Thu, 16 Apr 2020 02:34:08: 33000000 INFO @ Thu, 16 Apr 2020 02:34:12: 52000000 INFO @ Thu, 16 Apr 2020 02:34:14: 46000000 INFO @ Thu, 16 Apr 2020 02:34:15: 34000000 INFO @ Thu, 16 Apr 2020 02:34:17: 53000000 INFO @ Thu, 16 Apr 2020 02:34:19: 47000000 INFO @ Thu, 16 Apr 2020 02:34:21: 35000000 INFO @ Thu, 16 Apr 2020 02:34:22: #1 tag size is determined as 94 bps INFO @ Thu, 16 Apr 2020 02:34:22: #1 tag size = 94 INFO @ Thu, 16 Apr 2020 02:34:22: #1 total tags in treatment: 25245895 INFO @ Thu, 16 Apr 2020 02:34:22: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 02:34:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 02:34:22: #1 tags after filtering in treatment: 20768651 INFO @ Thu, 16 Apr 2020 02:34:22: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 02:34:22: #1 finished! INFO @ Thu, 16 Apr 2020 02:34:22: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 02:34:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 02:34:23: #2 number of paired peaks: 250 WARNING @ Thu, 16 Apr 2020 02:34:23: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Thu, 16 Apr 2020 02:34:23: start model_add_line... INFO @ Thu, 16 Apr 2020 02:34:23: start X-correlation... INFO @ Thu, 16 Apr 2020 02:34:24: end of X-cor INFO @ Thu, 16 Apr 2020 02:34:24: #2 finished! INFO @ Thu, 16 Apr 2020 02:34:24: #2 predicted fragment length is 116 bps INFO @ Thu, 16 Apr 2020 02:34:24: #2 alternative fragment length(s) may be 116 bps INFO @ Thu, 16 Apr 2020 02:34:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.05_model.r WARNING @ Thu, 16 Apr 2020 02:34:24: #2 Since the d (116) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 02:34:24: #2 You may need to consider one of the other alternative d(s): 116 WARNING @ Thu, 16 Apr 2020 02:34:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 02:34:24: #3 Call peaks... INFO @ Thu, 16 Apr 2020 02:34:24: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 02:34:24: 48000000 INFO @ Thu, 16 Apr 2020 02:34:27: 36000000 INFO @ Thu, 16 Apr 2020 02:34:29: 49000000 INFO @ Thu, 16 Apr 2020 02:34:33: 37000000 INFO @ Thu, 16 Apr 2020 02:34:35: 50000000 INFO @ Thu, 16 Apr 2020 02:34:40: 38000000 INFO @ Thu, 16 Apr 2020 02:34:40: 51000000 INFO @ Thu, 16 Apr 2020 02:34:45: 52000000 INFO @ Thu, 16 Apr 2020 02:34:45: 39000000 INFO @ Thu, 16 Apr 2020 02:34:50: 53000000 INFO @ Thu, 16 Apr 2020 02:34:51: 40000000 INFO @ Thu, 16 Apr 2020 02:34:55: #1 tag size is determined as 94 bps INFO @ Thu, 16 Apr 2020 02:34:55: #1 tag size = 94 INFO @ Thu, 16 Apr 2020 02:34:55: #1 total tags in treatment: 25245895 INFO @ Thu, 16 Apr 2020 02:34:55: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 02:34:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 02:34:55: #1 tags after filtering in treatment: 20768651 INFO @ Thu, 16 Apr 2020 02:34:55: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 02:34:55: #1 finished! INFO @ Thu, 16 Apr 2020 02:34:55: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 02:34:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 02:34:57: #2 number of paired peaks: 250 WARNING @ Thu, 16 Apr 2020 02:34:57: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Thu, 16 Apr 2020 02:34:57: start model_add_line... INFO @ Thu, 16 Apr 2020 02:34:57: start X-correlation... INFO @ Thu, 16 Apr 2020 02:34:57: end of X-cor INFO @ Thu, 16 Apr 2020 02:34:57: #2 finished! INFO @ Thu, 16 Apr 2020 02:34:57: #2 predicted fragment length is 116 bps INFO @ Thu, 16 Apr 2020 02:34:57: #2 alternative fragment length(s) may be 116 bps INFO @ Thu, 16 Apr 2020 02:34:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.10_model.r WARNING @ Thu, 16 Apr 2020 02:34:57: #2 Since the d (116) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 02:34:57: #2 You may need to consider one of the other alternative d(s): 116 WARNING @ Thu, 16 Apr 2020 02:34:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 02:34:57: #3 Call peaks... INFO @ Thu, 16 Apr 2020 02:34:57: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 02:34:57: 41000000 INFO @ Thu, 16 Apr 2020 02:35:03: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 02:35:03: 42000000 INFO @ Thu, 16 Apr 2020 02:35:09: 43000000 INFO @ Thu, 16 Apr 2020 02:35:15: 44000000 INFO @ Thu, 16 Apr 2020 02:35:21: 45000000 INFO @ Thu, 16 Apr 2020 02:35:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.05_peaks.xls INFO @ Thu, 16 Apr 2020 02:35:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 02:35:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.05_summits.bed INFO @ Thu, 16 Apr 2020 02:35:23: Done! INFO @ Thu, 16 Apr 2020 02:35:27: 46000000 pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (11790 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 02:35:33: 47000000 INFO @ Thu, 16 Apr 2020 02:35:36: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 02:35:39: 48000000 INFO @ Thu, 16 Apr 2020 02:35:46: 49000000 INFO @ Thu, 16 Apr 2020 02:35:52: 50000000 INFO @ Thu, 16 Apr 2020 02:35:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.10_peaks.xls INFO @ Thu, 16 Apr 2020 02:35:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 02:35:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.10_summits.bed INFO @ Thu, 16 Apr 2020 02:35:56: Done! INFO @ Thu, 16 Apr 2020 02:35:58: 51000000 pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6539 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 02:36:04: 52000000 INFO @ Thu, 16 Apr 2020 02:36:10: 53000000 INFO @ Thu, 16 Apr 2020 02:36:15: #1 tag size is determined as 94 bps INFO @ Thu, 16 Apr 2020 02:36:15: #1 tag size = 94 INFO @ Thu, 16 Apr 2020 02:36:15: #1 total tags in treatment: 25245895 INFO @ Thu, 16 Apr 2020 02:36:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 02:36:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 02:36:15: #1 tags after filtering in treatment: 20768651 INFO @ Thu, 16 Apr 2020 02:36:15: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 02:36:15: #1 finished! INFO @ Thu, 16 Apr 2020 02:36:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 02:36:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 02:36:16: #2 number of paired peaks: 250 WARNING @ Thu, 16 Apr 2020 02:36:16: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Thu, 16 Apr 2020 02:36:16: start model_add_line... INFO @ Thu, 16 Apr 2020 02:36:17: start X-correlation... INFO @ Thu, 16 Apr 2020 02:36:17: end of X-cor INFO @ Thu, 16 Apr 2020 02:36:17: #2 finished! INFO @ Thu, 16 Apr 2020 02:36:17: #2 predicted fragment length is 116 bps INFO @ Thu, 16 Apr 2020 02:36:17: #2 alternative fragment length(s) may be 116 bps INFO @ Thu, 16 Apr 2020 02:36:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.20_model.r WARNING @ Thu, 16 Apr 2020 02:36:17: #2 Since the d (116) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 02:36:17: #2 You may need to consider one of the other alternative d(s): 116 WARNING @ Thu, 16 Apr 2020 02:36:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 02:36:17: #3 Call peaks... INFO @ Thu, 16 Apr 2020 02:36:17: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 02:36:55: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 02:37:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.20_peaks.xls INFO @ Thu, 16 Apr 2020 02:37:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 02:37:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX1051947/ERX1051947.20_summits.bed INFO @ Thu, 16 Apr 2020 02:37:15: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2571 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。