Job ID = 1293328 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,881,776 reads read : 25,881,776 reads written : 25,881,776 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:17 25881776 reads; of these: 25881776 (100.00%) were unpaired; of these: 1768625 (6.83%) aligned 0 times 22271980 (86.05%) aligned exactly 1 time 1841171 (7.11%) aligned >1 times 93.17% overall alignment rate Time searching: 00:08:17 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3490761 / 24113151 = 0.1448 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 23:31:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:31:26: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:31:26: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:31:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:31:26: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:31:26: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:31:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:31:26: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:31:26: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:31:36: 1000000 INFO @ Sun, 02 Jun 2019 23:31:37: 1000000 INFO @ Sun, 02 Jun 2019 23:31:38: 1000000 INFO @ Sun, 02 Jun 2019 23:31:46: 2000000 INFO @ Sun, 02 Jun 2019 23:31:48: 2000000 INFO @ Sun, 02 Jun 2019 23:31:50: 2000000 INFO @ Sun, 02 Jun 2019 23:31:55: 3000000 INFO @ Sun, 02 Jun 2019 23:31:58: 3000000 INFO @ Sun, 02 Jun 2019 23:32:01: 3000000 INFO @ Sun, 02 Jun 2019 23:32:04: 4000000 INFO @ Sun, 02 Jun 2019 23:32:09: 4000000 INFO @ Sun, 02 Jun 2019 23:32:12: 4000000 INFO @ Sun, 02 Jun 2019 23:32:14: 5000000 INFO @ Sun, 02 Jun 2019 23:32:19: 5000000 INFO @ Sun, 02 Jun 2019 23:32:22: 5000000 INFO @ Sun, 02 Jun 2019 23:32:23: 6000000 INFO @ Sun, 02 Jun 2019 23:32:29: 6000000 INFO @ Sun, 02 Jun 2019 23:32:33: 6000000 INFO @ Sun, 02 Jun 2019 23:32:33: 7000000 INFO @ Sun, 02 Jun 2019 23:32:39: 7000000 INFO @ Sun, 02 Jun 2019 23:32:43: 8000000 INFO @ Sun, 02 Jun 2019 23:32:44: 7000000 INFO @ Sun, 02 Jun 2019 23:32:49: 8000000 INFO @ Sun, 02 Jun 2019 23:32:52: 9000000 INFO @ Sun, 02 Jun 2019 23:32:54: 8000000 INFO @ Sun, 02 Jun 2019 23:32:59: 9000000 INFO @ Sun, 02 Jun 2019 23:33:02: 10000000 INFO @ Sun, 02 Jun 2019 23:33:05: 9000000 INFO @ Sun, 02 Jun 2019 23:33:09: 10000000 INFO @ Sun, 02 Jun 2019 23:33:11: 11000000 INFO @ Sun, 02 Jun 2019 23:33:16: 10000000 INFO @ Sun, 02 Jun 2019 23:33:18: 11000000 INFO @ Sun, 02 Jun 2019 23:33:21: 12000000 INFO @ Sun, 02 Jun 2019 23:33:27: 11000000 INFO @ Sun, 02 Jun 2019 23:33:29: 12000000 INFO @ Sun, 02 Jun 2019 23:33:30: 13000000 INFO @ Sun, 02 Jun 2019 23:33:37: 12000000 INFO @ Sun, 02 Jun 2019 23:33:39: 13000000 INFO @ Sun, 02 Jun 2019 23:33:40: 14000000 INFO @ Sun, 02 Jun 2019 23:33:48: 13000000 INFO @ Sun, 02 Jun 2019 23:33:49: 14000000 INFO @ Sun, 02 Jun 2019 23:33:49: 15000000 INFO @ Sun, 02 Jun 2019 23:33:58: 16000000 INFO @ Sun, 02 Jun 2019 23:33:58: 14000000 INFO @ Sun, 02 Jun 2019 23:33:59: 15000000 INFO @ Sun, 02 Jun 2019 23:34:08: 17000000 INFO @ Sun, 02 Jun 2019 23:34:09: 16000000 INFO @ Sun, 02 Jun 2019 23:34:09: 15000000 INFO @ Sun, 02 Jun 2019 23:34:17: 18000000 INFO @ Sun, 02 Jun 2019 23:34:19: 17000000 INFO @ Sun, 02 Jun 2019 23:34:20: 16000000 INFO @ Sun, 02 Jun 2019 23:34:26: 19000000 INFO @ Sun, 02 Jun 2019 23:34:29: 18000000 INFO @ Sun, 02 Jun 2019 23:34:30: 17000000 INFO @ Sun, 02 Jun 2019 23:34:35: 20000000 INFO @ Sun, 02 Jun 2019 23:34:39: 19000000 INFO @ Sun, 02 Jun 2019 23:34:41: 18000000 INFO @ Sun, 02 Jun 2019 23:34:41: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:34:41: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:34:41: #1 total tags in treatment: 20622390 INFO @ Sun, 02 Jun 2019 23:34:41: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:34:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:34:41: #1 tags after filtering in treatment: 20622390 INFO @ Sun, 02 Jun 2019 23:34:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:34:41: #1 finished! INFO @ Sun, 02 Jun 2019 23:34:41: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:34:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:34:43: #2 number of paired peaks: 6 WARNING @ Sun, 02 Jun 2019 23:34:43: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:34:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:34:49: 20000000 INFO @ Sun, 02 Jun 2019 23:34:51: 19000000 INFO @ Sun, 02 Jun 2019 23:34:55: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:34:55: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:34:55: #1 total tags in treatment: 20622390 INFO @ Sun, 02 Jun 2019 23:34:55: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:34:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:34:56: #1 tags after filtering in treatment: 20622390 INFO @ Sun, 02 Jun 2019 23:34:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:34:56: #1 finished! INFO @ Sun, 02 Jun 2019 23:34:56: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:34:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:34:58: #2 number of paired peaks: 6 WARNING @ Sun, 02 Jun 2019 23:34:58: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:34:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:35:02: 20000000 INFO @ Sun, 02 Jun 2019 23:35:08: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:35:08: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:35:08: #1 total tags in treatment: 20622390 INFO @ Sun, 02 Jun 2019 23:35:08: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:35:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:35:08: #1 tags after filtering in treatment: 20622390 INFO @ Sun, 02 Jun 2019 23:35:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:35:08: #1 finished! INFO @ Sun, 02 Jun 2019 23:35:08: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:35:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:35:10: #2 number of paired peaks: 6 WARNING @ Sun, 02 Jun 2019 23:35:10: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:35:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102379/ERX102379.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。