Job ID = 1293323 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,936,578 reads read : 25,936,578 reads written : 25,936,578 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:10 25936578 reads; of these: 25936578 (100.00%) were unpaired; of these: 2010958 (7.75%) aligned 0 times 21730415 (83.78%) aligned exactly 1 time 2195205 (8.46%) aligned >1 times 92.25% overall alignment rate Time searching: 00:09:10 Overall time: 00:09:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3137261 / 23925620 = 0.1311 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 23:13:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:13:38: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:13:38: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:13:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:13:38: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:13:38: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:13:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:13:38: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:13:38: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:13:46: 1000000 INFO @ Sun, 02 Jun 2019 23:13:47: 1000000 INFO @ Sun, 02 Jun 2019 23:13:48: 1000000 INFO @ Sun, 02 Jun 2019 23:13:54: 2000000 INFO @ Sun, 02 Jun 2019 23:13:56: 2000000 INFO @ Sun, 02 Jun 2019 23:13:58: 2000000 INFO @ Sun, 02 Jun 2019 23:14:01: 3000000 INFO @ Sun, 02 Jun 2019 23:14:04: 3000000 INFO @ Sun, 02 Jun 2019 23:14:08: 3000000 INFO @ Sun, 02 Jun 2019 23:14:08: 4000000 INFO @ Sun, 02 Jun 2019 23:14:12: 4000000 INFO @ Sun, 02 Jun 2019 23:14:16: 5000000 INFO @ Sun, 02 Jun 2019 23:14:17: 4000000 INFO @ Sun, 02 Jun 2019 23:14:20: 5000000 INFO @ Sun, 02 Jun 2019 23:14:23: 6000000 INFO @ Sun, 02 Jun 2019 23:14:27: 5000000 INFO @ Sun, 02 Jun 2019 23:14:29: 6000000 INFO @ Sun, 02 Jun 2019 23:14:31: 7000000 INFO @ Sun, 02 Jun 2019 23:14:37: 6000000 INFO @ Sun, 02 Jun 2019 23:14:37: 7000000 INFO @ Sun, 02 Jun 2019 23:14:38: 8000000 INFO @ Sun, 02 Jun 2019 23:14:45: 8000000 INFO @ Sun, 02 Jun 2019 23:14:46: 9000000 INFO @ Sun, 02 Jun 2019 23:14:46: 7000000 INFO @ Sun, 02 Jun 2019 23:14:53: 10000000 INFO @ Sun, 02 Jun 2019 23:14:53: 9000000 INFO @ Sun, 02 Jun 2019 23:14:55: 8000000 INFO @ Sun, 02 Jun 2019 23:15:01: 11000000 INFO @ Sun, 02 Jun 2019 23:15:01: 10000000 INFO @ Sun, 02 Jun 2019 23:15:05: 9000000 INFO @ Sun, 02 Jun 2019 23:15:08: 12000000 INFO @ Sun, 02 Jun 2019 23:15:09: 11000000 INFO @ Sun, 02 Jun 2019 23:15:14: 10000000 INFO @ Sun, 02 Jun 2019 23:15:15: 13000000 INFO @ Sun, 02 Jun 2019 23:15:18: 12000000 INFO @ Sun, 02 Jun 2019 23:15:23: 14000000 INFO @ Sun, 02 Jun 2019 23:15:25: 11000000 INFO @ Sun, 02 Jun 2019 23:15:26: 13000000 INFO @ Sun, 02 Jun 2019 23:15:30: 15000000 INFO @ Sun, 02 Jun 2019 23:15:34: 14000000 INFO @ Sun, 02 Jun 2019 23:15:34: 12000000 INFO @ Sun, 02 Jun 2019 23:15:38: 16000000 INFO @ Sun, 02 Jun 2019 23:15:42: 15000000 INFO @ Sun, 02 Jun 2019 23:15:44: 13000000 INFO @ Sun, 02 Jun 2019 23:15:45: 17000000 INFO @ Sun, 02 Jun 2019 23:15:50: 16000000 INFO @ Sun, 02 Jun 2019 23:15:52: 18000000 INFO @ Sun, 02 Jun 2019 23:15:53: 14000000 INFO @ Sun, 02 Jun 2019 23:15:58: 17000000 INFO @ Sun, 02 Jun 2019 23:16:00: 19000000 INFO @ Sun, 02 Jun 2019 23:16:03: 15000000 INFO @ Sun, 02 Jun 2019 23:16:07: 18000000 INFO @ Sun, 02 Jun 2019 23:16:07: 20000000 INFO @ Sun, 02 Jun 2019 23:16:12: 16000000 INFO @ Sun, 02 Jun 2019 23:16:13: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:16:13: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:16:13: #1 total tags in treatment: 20788359 INFO @ Sun, 02 Jun 2019 23:16:13: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:16:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:16:14: #1 tags after filtering in treatment: 20788359 INFO @ Sun, 02 Jun 2019 23:16:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:16:14: #1 finished! INFO @ Sun, 02 Jun 2019 23:16:14: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:16:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:16:15: 19000000 INFO @ Sun, 02 Jun 2019 23:16:15: #2 number of paired peaks: 19 WARNING @ Sun, 02 Jun 2019 23:16:15: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:16:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:16:21: 17000000 INFO @ Sun, 02 Jun 2019 23:16:23: 20000000 INFO @ Sun, 02 Jun 2019 23:16:29: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:16:29: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:16:29: #1 total tags in treatment: 20788359 INFO @ Sun, 02 Jun 2019 23:16:29: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:16:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:16:30: #1 tags after filtering in treatment: 20788359 INFO @ Sun, 02 Jun 2019 23:16:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:16:30: #1 finished! INFO @ Sun, 02 Jun 2019 23:16:30: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:16:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:16:31: 18000000 INFO @ Sun, 02 Jun 2019 23:16:31: #2 number of paired peaks: 19 WARNING @ Sun, 02 Jun 2019 23:16:31: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:16:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:16:39: 19000000 INFO @ Sun, 02 Jun 2019 23:16:48: 20000000 INFO @ Sun, 02 Jun 2019 23:16:54: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:16:54: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:16:54: #1 total tags in treatment: 20788359 INFO @ Sun, 02 Jun 2019 23:16:54: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:16:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:16:54: #1 tags after filtering in treatment: 20788359 INFO @ Sun, 02 Jun 2019 23:16:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:16:54: #1 finished! INFO @ Sun, 02 Jun 2019 23:16:54: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:16:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:16:56: #2 number of paired peaks: 19 WARNING @ Sun, 02 Jun 2019 23:16:56: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:16:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102374/ERX102374.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。