Job ID = 1293312 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,564,190 reads read : 13,564,190 reads written : 13,564,190 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:23 13564190 reads; of these: 13564190 (100.00%) were unpaired; of these: 857395 (6.32%) aligned 0 times 11676482 (86.08%) aligned exactly 1 time 1030313 (7.60%) aligned >1 times 93.68% overall alignment rate Time searching: 00:03:23 Overall time: 00:03:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1381353 / 12706795 = 0.1087 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 23:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:02:46: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:02:46: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:02:46: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:02:46: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:02:46: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:02:46: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:02:56: 1000000 INFO @ Sun, 02 Jun 2019 23:02:57: 1000000 INFO @ Sun, 02 Jun 2019 23:02:57: 1000000 INFO @ Sun, 02 Jun 2019 23:03:06: 2000000 INFO @ Sun, 02 Jun 2019 23:03:08: 2000000 INFO @ Sun, 02 Jun 2019 23:03:08: 2000000 INFO @ Sun, 02 Jun 2019 23:03:16: 3000000 INFO @ Sun, 02 Jun 2019 23:03:18: 3000000 INFO @ Sun, 02 Jun 2019 23:03:20: 3000000 INFO @ Sun, 02 Jun 2019 23:03:25: 4000000 INFO @ Sun, 02 Jun 2019 23:03:28: 4000000 INFO @ Sun, 02 Jun 2019 23:03:30: 4000000 INFO @ Sun, 02 Jun 2019 23:03:35: 5000000 INFO @ Sun, 02 Jun 2019 23:03:38: 5000000 INFO @ Sun, 02 Jun 2019 23:03:41: 5000000 INFO @ Sun, 02 Jun 2019 23:03:44: 6000000 INFO @ Sun, 02 Jun 2019 23:03:48: 6000000 INFO @ Sun, 02 Jun 2019 23:03:52: 6000000 INFO @ Sun, 02 Jun 2019 23:03:54: 7000000 INFO @ Sun, 02 Jun 2019 23:03:58: 7000000 INFO @ Sun, 02 Jun 2019 23:04:02: 7000000 INFO @ Sun, 02 Jun 2019 23:04:03: 8000000 INFO @ Sun, 02 Jun 2019 23:04:08: 8000000 INFO @ Sun, 02 Jun 2019 23:04:13: 8000000 INFO @ Sun, 02 Jun 2019 23:04:13: 9000000 INFO @ Sun, 02 Jun 2019 23:04:17: 9000000 INFO @ Sun, 02 Jun 2019 23:04:22: 10000000 INFO @ Sun, 02 Jun 2019 23:04:23: 9000000 INFO @ Sun, 02 Jun 2019 23:04:27: 10000000 INFO @ Sun, 02 Jun 2019 23:04:31: 11000000 INFO @ Sun, 02 Jun 2019 23:04:34: 10000000 INFO @ Sun, 02 Jun 2019 23:04:34: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:04:34: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:04:34: #1 total tags in treatment: 11325442 INFO @ Sun, 02 Jun 2019 23:04:34: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:04:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:04:35: #1 tags after filtering in treatment: 11325442 INFO @ Sun, 02 Jun 2019 23:04:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:04:35: #1 finished! INFO @ Sun, 02 Jun 2019 23:04:35: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:04:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:04:36: #2 number of paired peaks: 21 WARNING @ Sun, 02 Jun 2019 23:04:36: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:04:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:04:37: 11000000 INFO @ Sun, 02 Jun 2019 23:04:40: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:04:40: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:04:40: #1 total tags in treatment: 11325442 INFO @ Sun, 02 Jun 2019 23:04:40: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:04:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:04:40: #1 tags after filtering in treatment: 11325442 INFO @ Sun, 02 Jun 2019 23:04:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:04:40: #1 finished! INFO @ Sun, 02 Jun 2019 23:04:40: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:04:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:04:41: #2 number of paired peaks: 21 WARNING @ Sun, 02 Jun 2019 23:04:41: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:04:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:04:44: 11000000 INFO @ Sun, 02 Jun 2019 23:04:47: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:04:47: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:04:47: #1 total tags in treatment: 11325442 INFO @ Sun, 02 Jun 2019 23:04:47: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:04:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:04:47: #1 tags after filtering in treatment: 11325442 INFO @ Sun, 02 Jun 2019 23:04:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:04:47: #1 finished! INFO @ Sun, 02 Jun 2019 23:04:47: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:04:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:04:48: #2 number of paired peaks: 21 WARNING @ Sun, 02 Jun 2019 23:04:48: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:04:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101811/ERX101811.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。