Job ID = 6626449 SRX = ERX088874 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 166977586 spots for ERR111437/ERR111437.sra Written 166977586 spots for ERR111437/ERR111437.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626727 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:39:26 166977586 reads; of these: 166977586 (100.00%) were unpaired; of these: 7226710 (4.33%) aligned 0 times 133627651 (80.03%) aligned exactly 1 time 26123225 (15.64%) aligned >1 times 95.67% overall alignment rate Time searching: 00:39:27 Overall time: 00:39:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 108647888 / 159750876 = 0.6801 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:20:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:20:34: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:20:34: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:20:39: 1000000 INFO @ Tue, 14 Jul 2020 08:20:45: 2000000 INFO @ Tue, 14 Jul 2020 08:20:50: 3000000 INFO @ Tue, 14 Jul 2020 08:20:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:21:02: 5000000 INFO @ Tue, 14 Jul 2020 08:21:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:21:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:21:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:21:08: 6000000 INFO @ Tue, 14 Jul 2020 08:21:09: 1000000 INFO @ Tue, 14 Jul 2020 08:21:14: 7000000 INFO @ Tue, 14 Jul 2020 08:21:15: 2000000 INFO @ Tue, 14 Jul 2020 08:21:21: 8000000 INFO @ Tue, 14 Jul 2020 08:21:21: 3000000 INFO @ Tue, 14 Jul 2020 08:21:27: 9000000 INFO @ Tue, 14 Jul 2020 08:21:28: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:21:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:21:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:21:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:21:34: 10000000 INFO @ Tue, 14 Jul 2020 08:21:34: 5000000 INFO @ Tue, 14 Jul 2020 08:21:41: 1000000 INFO @ Tue, 14 Jul 2020 08:21:41: 11000000 INFO @ Tue, 14 Jul 2020 08:21:42: 6000000 INFO @ Tue, 14 Jul 2020 08:21:48: 12000000 INFO @ Tue, 14 Jul 2020 08:21:48: 2000000 INFO @ Tue, 14 Jul 2020 08:21:49: 7000000 INFO @ Tue, 14 Jul 2020 08:21:55: 13000000 INFO @ Tue, 14 Jul 2020 08:21:55: 3000000 INFO @ Tue, 14 Jul 2020 08:21:56: 8000000 INFO @ Tue, 14 Jul 2020 08:22:02: 14000000 INFO @ Tue, 14 Jul 2020 08:22:03: 9000000 INFO @ Tue, 14 Jul 2020 08:22:03: 4000000 INFO @ Tue, 14 Jul 2020 08:22:08: 15000000 INFO @ Tue, 14 Jul 2020 08:22:10: 10000000 INFO @ Tue, 14 Jul 2020 08:22:10: 5000000 INFO @ Tue, 14 Jul 2020 08:22:15: 16000000 INFO @ Tue, 14 Jul 2020 08:22:16: 11000000 INFO @ Tue, 14 Jul 2020 08:22:18: 6000000 INFO @ Tue, 14 Jul 2020 08:22:22: 17000000 INFO @ Tue, 14 Jul 2020 08:22:23: 12000000 INFO @ Tue, 14 Jul 2020 08:22:26: 7000000 INFO @ Tue, 14 Jul 2020 08:22:29: 18000000 INFO @ Tue, 14 Jul 2020 08:22:30: 13000000 INFO @ Tue, 14 Jul 2020 08:22:33: 8000000 INFO @ Tue, 14 Jul 2020 08:22:36: 19000000 INFO @ Tue, 14 Jul 2020 08:22:37: 14000000 INFO @ Tue, 14 Jul 2020 08:22:40: 9000000 INFO @ Tue, 14 Jul 2020 08:22:43: 20000000 INFO @ Tue, 14 Jul 2020 08:22:44: 15000000 INFO @ Tue, 14 Jul 2020 08:22:48: 10000000 INFO @ Tue, 14 Jul 2020 08:22:50: 21000000 INFO @ Tue, 14 Jul 2020 08:22:51: 16000000 INFO @ Tue, 14 Jul 2020 08:22:55: 11000000 INFO @ Tue, 14 Jul 2020 08:22:57: 22000000 INFO @ Tue, 14 Jul 2020 08:22:58: 17000000 INFO @ Tue, 14 Jul 2020 08:23:02: 12000000 INFO @ Tue, 14 Jul 2020 08:23:04: 23000000 INFO @ Tue, 14 Jul 2020 08:23:05: 18000000 INFO @ Tue, 14 Jul 2020 08:23:09: 13000000 INFO @ Tue, 14 Jul 2020 08:23:10: 24000000 INFO @ Tue, 14 Jul 2020 08:23:12: 19000000 INFO @ Tue, 14 Jul 2020 08:23:16: 14000000 INFO @ Tue, 14 Jul 2020 08:23:17: 25000000 INFO @ Tue, 14 Jul 2020 08:23:19: 20000000 INFO @ Tue, 14 Jul 2020 08:23:23: 15000000 INFO @ Tue, 14 Jul 2020 08:23:24: 26000000 INFO @ Tue, 14 Jul 2020 08:23:25: 21000000 INFO @ Tue, 14 Jul 2020 08:23:31: 16000000 INFO @ Tue, 14 Jul 2020 08:23:31: 27000000 INFO @ Tue, 14 Jul 2020 08:23:32: 22000000 INFO @ Tue, 14 Jul 2020 08:23:38: 17000000 INFO @ Tue, 14 Jul 2020 08:23:38: 28000000 INFO @ Tue, 14 Jul 2020 08:23:39: 23000000 INFO @ Tue, 14 Jul 2020 08:23:45: 18000000 INFO @ Tue, 14 Jul 2020 08:23:45: 29000000 INFO @ Tue, 14 Jul 2020 08:23:46: 24000000 INFO @ Tue, 14 Jul 2020 08:23:52: 19000000 INFO @ Tue, 14 Jul 2020 08:23:52: 30000000 INFO @ Tue, 14 Jul 2020 08:23:53: 25000000 INFO @ Tue, 14 Jul 2020 08:23:59: 20000000 INFO @ Tue, 14 Jul 2020 08:23:59: 31000000 INFO @ Tue, 14 Jul 2020 08:24:00: 26000000 INFO @ Tue, 14 Jul 2020 08:24:06: 32000000 INFO @ Tue, 14 Jul 2020 08:24:06: 21000000 INFO @ Tue, 14 Jul 2020 08:24:07: 27000000 INFO @ Tue, 14 Jul 2020 08:24:13: 33000000 INFO @ Tue, 14 Jul 2020 08:24:13: 22000000 INFO @ Tue, 14 Jul 2020 08:24:13: 28000000 INFO @ Tue, 14 Jul 2020 08:24:19: 34000000 INFO @ Tue, 14 Jul 2020 08:24:20: 29000000 INFO @ Tue, 14 Jul 2020 08:24:20: 23000000 INFO @ Tue, 14 Jul 2020 08:24:26: 35000000 INFO @ Tue, 14 Jul 2020 08:24:27: 30000000 INFO @ Tue, 14 Jul 2020 08:24:27: 24000000 INFO @ Tue, 14 Jul 2020 08:24:33: 36000000 INFO @ Tue, 14 Jul 2020 08:24:33: 31000000 INFO @ Tue, 14 Jul 2020 08:24:35: 25000000 INFO @ Tue, 14 Jul 2020 08:24:39: 37000000 INFO @ Tue, 14 Jul 2020 08:24:40: 32000000 INFO @ Tue, 14 Jul 2020 08:24:42: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:24:46: 38000000 INFO @ Tue, 14 Jul 2020 08:24:47: 33000000 INFO @ Tue, 14 Jul 2020 08:24:49: 27000000 INFO @ Tue, 14 Jul 2020 08:24:53: 39000000 INFO @ Tue, 14 Jul 2020 08:24:53: 34000000 INFO @ Tue, 14 Jul 2020 08:24:56: 28000000 INFO @ Tue, 14 Jul 2020 08:24:59: 40000000 INFO @ Tue, 14 Jul 2020 08:25:00: 35000000 INFO @ Tue, 14 Jul 2020 08:25:03: 29000000 INFO @ Tue, 14 Jul 2020 08:25:06: 41000000 INFO @ Tue, 14 Jul 2020 08:25:07: 36000000 INFO @ Tue, 14 Jul 2020 08:25:10: 30000000 INFO @ Tue, 14 Jul 2020 08:25:13: 37000000 INFO @ Tue, 14 Jul 2020 08:25:13: 42000000 INFO @ Tue, 14 Jul 2020 08:25:17: 31000000 INFO @ Tue, 14 Jul 2020 08:25:20: 38000000 INFO @ Tue, 14 Jul 2020 08:25:20: 43000000 INFO @ Tue, 14 Jul 2020 08:25:24: 32000000 INFO @ Tue, 14 Jul 2020 08:25:26: 39000000 INFO @ Tue, 14 Jul 2020 08:25:27: 44000000 INFO @ Tue, 14 Jul 2020 08:25:31: 33000000 INFO @ Tue, 14 Jul 2020 08:25:33: 40000000 INFO @ Tue, 14 Jul 2020 08:25:34: 45000000 INFO @ Tue, 14 Jul 2020 08:25:39: 34000000 INFO @ Tue, 14 Jul 2020 08:25:40: 41000000 INFO @ Tue, 14 Jul 2020 08:25:41: 46000000 INFO @ Tue, 14 Jul 2020 08:25:46: 35000000 INFO @ Tue, 14 Jul 2020 08:25:47: 42000000 INFO @ Tue, 14 Jul 2020 08:25:47: 47000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:25:53: 36000000 INFO @ Tue, 14 Jul 2020 08:25:54: 48000000 INFO @ Tue, 14 Jul 2020 08:25:54: 43000000 INFO @ Tue, 14 Jul 2020 08:26:00: 37000000 INFO @ Tue, 14 Jul 2020 08:26:01: 49000000 INFO @ Tue, 14 Jul 2020 08:26:01: 44000000 INFO @ Tue, 14 Jul 2020 08:26:07: 38000000 INFO @ Tue, 14 Jul 2020 08:26:07: 50000000 INFO @ Tue, 14 Jul 2020 08:26:08: 45000000 INFO @ Tue, 14 Jul 2020 08:26:14: 46000000 INFO @ Tue, 14 Jul 2020 08:26:14: 51000000 INFO @ Tue, 14 Jul 2020 08:26:15: 39000000 INFO @ Tue, 14 Jul 2020 08:26:15: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:26:15: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:26:15: #1 total tags in treatment: 51102988 INFO @ Tue, 14 Jul 2020 08:26:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:26:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:26:16: #1 tags after filtering in treatment: 51102988 INFO @ Tue, 14 Jul 2020 08:26:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:26:16: #1 finished! INFO @ Tue, 14 Jul 2020 08:26:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:26:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:26:19: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:26:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:26:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:26:21: 47000000 INFO @ Tue, 14 Jul 2020 08:26:22: 40000000 INFO @ Tue, 14 Jul 2020 08:26:27: 48000000 INFO @ Tue, 14 Jul 2020 08:26:29: 41000000 INFO @ Tue, 14 Jul 2020 08:26:34: 49000000 INFO @ Tue, 14 Jul 2020 08:26:36: 42000000 INFO @ Tue, 14 Jul 2020 08:26:41: 50000000 INFO @ Tue, 14 Jul 2020 08:26:43: 43000000 INFO @ Tue, 14 Jul 2020 08:26:47: 51000000 INFO @ Tue, 14 Jul 2020 08:26:48: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:26:48: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:26:48: #1 total tags in treatment: 51102988 INFO @ Tue, 14 Jul 2020 08:26:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:26:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:26:49: #1 tags after filtering in treatment: 51102988 INFO @ Tue, 14 Jul 2020 08:26:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:26:49: #1 finished! INFO @ Tue, 14 Jul 2020 08:26:49: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:26:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:26:50: 44000000 INFO @ Tue, 14 Jul 2020 08:26:52: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:26:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:26:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:26:57: 45000000 INFO @ Tue, 14 Jul 2020 08:27:04: 46000000 INFO @ Tue, 14 Jul 2020 08:27:11: 47000000 INFO @ Tue, 14 Jul 2020 08:27:18: 48000000 INFO @ Tue, 14 Jul 2020 08:27:25: 49000000 INFO @ Tue, 14 Jul 2020 08:27:32: 50000000 INFO @ Tue, 14 Jul 2020 08:27:38: 51000000 INFO @ Tue, 14 Jul 2020 08:27:40: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:27:40: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:27:40: #1 total tags in treatment: 51102988 INFO @ Tue, 14 Jul 2020 08:27:40: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:27:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:27:40: #1 tags after filtering in treatment: 51102988 INFO @ Tue, 14 Jul 2020 08:27:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:27:40: #1 finished! INFO @ Tue, 14 Jul 2020 08:27:40: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:27:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:27:43: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:27:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:27:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088874/ERX088874.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling