Job ID = 6626448 SRX = ERX088873 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 169561362 spots for ERR111441/ERR111441.sra Written 169561362 spots for ERR111441/ERR111441.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626736 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:42:12 169561362 reads; of these: 169561362 (100.00%) were unpaired; of these: 4488591 (2.65%) aligned 0 times 135695091 (80.03%) aligned exactly 1 time 29377680 (17.33%) aligned >1 times 97.35% overall alignment rate Time searching: 00:42:12 Overall time: 00:42:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 114100511 / 165072771 = 0.6912 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:24:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:24:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:24:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:24:46: 1000000 INFO @ Tue, 14 Jul 2020 08:24:51: 2000000 INFO @ Tue, 14 Jul 2020 08:24:57: 3000000 INFO @ Tue, 14 Jul 2020 08:25:03: 4000000 INFO @ Tue, 14 Jul 2020 08:25:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:14: 6000000 INFO @ Tue, 14 Jul 2020 08:25:16: 1000000 INFO @ Tue, 14 Jul 2020 08:25:20: 7000000 INFO @ Tue, 14 Jul 2020 08:25:22: 2000000 INFO @ Tue, 14 Jul 2020 08:25:26: 8000000 INFO @ Tue, 14 Jul 2020 08:25:28: 3000000 INFO @ Tue, 14 Jul 2020 08:25:32: 9000000 INFO @ Tue, 14 Jul 2020 08:25:34: 4000000 INFO @ Tue, 14 Jul 2020 08:25:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:40: 5000000 INFO @ Tue, 14 Jul 2020 08:25:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:44: 11000000 INFO @ Tue, 14 Jul 2020 08:25:46: 6000000 INFO @ Tue, 14 Jul 2020 08:25:46: 1000000 INFO @ Tue, 14 Jul 2020 08:25:50: 12000000 INFO @ Tue, 14 Jul 2020 08:25:52: 7000000 INFO @ Tue, 14 Jul 2020 08:25:52: 2000000 INFO @ Tue, 14 Jul 2020 08:25:56: 13000000 INFO @ Tue, 14 Jul 2020 08:25:58: 8000000 INFO @ Tue, 14 Jul 2020 08:25:58: 3000000 INFO @ Tue, 14 Jul 2020 08:26:02: 14000000 INFO @ Tue, 14 Jul 2020 08:26:04: 9000000 INFO @ Tue, 14 Jul 2020 08:26:04: 4000000 INFO @ Tue, 14 Jul 2020 08:26:09: 15000000 INFO @ Tue, 14 Jul 2020 08:26:10: 10000000 INFO @ Tue, 14 Jul 2020 08:26:10: 5000000 INFO @ Tue, 14 Jul 2020 08:26:15: 16000000 INFO @ Tue, 14 Jul 2020 08:26:15: 11000000 INFO @ Tue, 14 Jul 2020 08:26:16: 6000000 INFO @ Tue, 14 Jul 2020 08:26:21: 17000000 INFO @ Tue, 14 Jul 2020 08:26:21: 12000000 INFO @ Tue, 14 Jul 2020 08:26:23: 7000000 INFO @ Tue, 14 Jul 2020 08:26:27: 18000000 INFO @ Tue, 14 Jul 2020 08:26:27: 13000000 INFO @ Tue, 14 Jul 2020 08:26:29: 8000000 INFO @ Tue, 14 Jul 2020 08:26:33: 19000000 INFO @ Tue, 14 Jul 2020 08:26:33: 14000000 INFO @ Tue, 14 Jul 2020 08:26:35: 9000000 INFO @ Tue, 14 Jul 2020 08:26:39: 20000000 INFO @ Tue, 14 Jul 2020 08:26:39: 15000000 INFO @ Tue, 14 Jul 2020 08:26:41: 10000000 INFO @ Tue, 14 Jul 2020 08:26:45: 21000000 INFO @ Tue, 14 Jul 2020 08:26:46: 16000000 INFO @ Tue, 14 Jul 2020 08:26:47: 11000000 INFO @ Tue, 14 Jul 2020 08:26:51: 22000000 INFO @ Tue, 14 Jul 2020 08:26:52: 17000000 INFO @ Tue, 14 Jul 2020 08:26:53: 12000000 INFO @ Tue, 14 Jul 2020 08:26:58: 23000000 INFO @ Tue, 14 Jul 2020 08:26:58: 18000000 INFO @ Tue, 14 Jul 2020 08:26:59: 13000000 INFO @ Tue, 14 Jul 2020 08:27:04: 19000000 INFO @ Tue, 14 Jul 2020 08:27:04: 24000000 INFO @ Tue, 14 Jul 2020 08:27:05: 14000000 INFO @ Tue, 14 Jul 2020 08:27:10: 20000000 INFO @ Tue, 14 Jul 2020 08:27:10: 25000000 INFO @ Tue, 14 Jul 2020 08:27:11: 15000000 INFO @ Tue, 14 Jul 2020 08:27:15: 21000000 INFO @ Tue, 14 Jul 2020 08:27:16: 26000000 INFO @ Tue, 14 Jul 2020 08:27:17: 16000000 INFO @ Tue, 14 Jul 2020 08:27:21: 22000000 INFO @ Tue, 14 Jul 2020 08:27:22: 27000000 INFO @ Tue, 14 Jul 2020 08:27:23: 17000000 INFO @ Tue, 14 Jul 2020 08:27:27: 23000000 INFO @ Tue, 14 Jul 2020 08:27:28: 28000000 INFO @ Tue, 14 Jul 2020 08:27:29: 18000000 INFO @ Tue, 14 Jul 2020 08:27:33: 24000000 INFO @ Tue, 14 Jul 2020 08:27:34: 29000000 INFO @ Tue, 14 Jul 2020 08:27:35: 19000000 INFO @ Tue, 14 Jul 2020 08:27:39: 25000000 INFO @ Tue, 14 Jul 2020 08:27:40: 30000000 INFO @ Tue, 14 Jul 2020 08:27:41: 20000000 INFO @ Tue, 14 Jul 2020 08:27:45: 26000000 INFO @ Tue, 14 Jul 2020 08:27:46: 31000000 INFO @ Tue, 14 Jul 2020 08:27:47: 21000000 INFO @ Tue, 14 Jul 2020 08:27:51: 27000000 INFO @ Tue, 14 Jul 2020 08:27:52: 32000000 INFO @ Tue, 14 Jul 2020 08:27:53: 22000000 INFO @ Tue, 14 Jul 2020 08:27:57: 28000000 INFO @ Tue, 14 Jul 2020 08:27:58: 33000000 INFO @ Tue, 14 Jul 2020 08:27:59: 23000000 INFO @ Tue, 14 Jul 2020 08:28:03: 29000000 INFO @ Tue, 14 Jul 2020 08:28:04: 34000000 INFO @ Tue, 14 Jul 2020 08:28:05: 24000000 INFO @ Tue, 14 Jul 2020 08:28:09: 30000000 INFO @ Tue, 14 Jul 2020 08:28:10: 35000000 INFO @ Tue, 14 Jul 2020 08:28:11: 25000000 INFO @ Tue, 14 Jul 2020 08:28:15: 31000000 INFO @ Tue, 14 Jul 2020 08:28:16: 36000000 INFO @ Tue, 14 Jul 2020 08:28:17: 26000000 INFO @ Tue, 14 Jul 2020 08:28:21: 32000000 INFO @ Tue, 14 Jul 2020 08:28:22: 37000000 INFO @ Tue, 14 Jul 2020 08:28:23: 27000000 INFO @ Tue, 14 Jul 2020 08:28:27: 33000000 INFO @ Tue, 14 Jul 2020 08:28:28: 38000000 INFO @ Tue, 14 Jul 2020 08:28:29: 28000000 INFO @ Tue, 14 Jul 2020 08:28:32: 34000000 INFO @ Tue, 14 Jul 2020 08:28:34: 39000000 INFO @ Tue, 14 Jul 2020 08:28:35: 29000000 INFO @ Tue, 14 Jul 2020 08:28:38: 35000000 INFO @ Tue, 14 Jul 2020 08:28:40: 40000000 INFO @ Tue, 14 Jul 2020 08:28:41: 30000000 INFO @ Tue, 14 Jul 2020 08:28:44: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:28:46: 41000000 INFO @ Tue, 14 Jul 2020 08:28:46: 31000000 INFO @ Tue, 14 Jul 2020 08:28:50: 37000000 INFO @ Tue, 14 Jul 2020 08:28:52: 42000000 INFO @ Tue, 14 Jul 2020 08:28:52: 32000000 INFO @ Tue, 14 Jul 2020 08:28:56: 38000000 INFO @ Tue, 14 Jul 2020 08:28:58: 43000000 INFO @ Tue, 14 Jul 2020 08:28:58: 33000000 INFO @ Tue, 14 Jul 2020 08:29:02: 39000000 INFO @ Tue, 14 Jul 2020 08:29:04: 44000000 INFO @ Tue, 14 Jul 2020 08:29:04: 34000000 INFO @ Tue, 14 Jul 2020 08:29:08: 40000000 INFO @ Tue, 14 Jul 2020 08:29:10: 45000000 INFO @ Tue, 14 Jul 2020 08:29:10: 35000000 INFO @ Tue, 14 Jul 2020 08:29:14: 41000000 INFO @ Tue, 14 Jul 2020 08:29:16: 46000000 INFO @ Tue, 14 Jul 2020 08:29:16: 36000000 INFO @ Tue, 14 Jul 2020 08:29:20: 42000000 INFO @ Tue, 14 Jul 2020 08:29:22: 37000000 INFO @ Tue, 14 Jul 2020 08:29:22: 47000000 INFO @ Tue, 14 Jul 2020 08:29:26: 43000000 INFO @ Tue, 14 Jul 2020 08:29:28: 48000000 INFO @ Tue, 14 Jul 2020 08:29:28: 38000000 INFO @ Tue, 14 Jul 2020 08:29:32: 44000000 INFO @ Tue, 14 Jul 2020 08:29:34: 49000000 INFO @ Tue, 14 Jul 2020 08:29:34: 39000000 INFO @ Tue, 14 Jul 2020 08:29:38: 45000000 INFO @ Tue, 14 Jul 2020 08:29:40: 50000000 INFO @ Tue, 14 Jul 2020 08:29:40: 40000000 INFO @ Tue, 14 Jul 2020 08:29:44: 46000000 INFO @ Tue, 14 Jul 2020 08:29:46: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:29:46: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:29:46: #1 total tags in treatment: 50972260 INFO @ Tue, 14 Jul 2020 08:29:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:29:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:29:46: 41000000 INFO @ Tue, 14 Jul 2020 08:29:47: #1 tags after filtering in treatment: 50972260 INFO @ Tue, 14 Jul 2020 08:29:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:29:47: #1 finished! INFO @ Tue, 14 Jul 2020 08:29:47: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:29:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:29:50: 47000000 INFO @ Tue, 14 Jul 2020 08:29:50: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:29:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:29:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:29:52: 42000000 INFO @ Tue, 14 Jul 2020 08:29:56: 48000000 INFO @ Tue, 14 Jul 2020 08:29:58: 43000000 INFO @ Tue, 14 Jul 2020 08:30:02: 49000000 INFO @ Tue, 14 Jul 2020 08:30:04: 44000000 INFO @ Tue, 14 Jul 2020 08:30:07: 50000000 INFO @ Tue, 14 Jul 2020 08:30:10: 45000000 INFO @ Tue, 14 Jul 2020 08:30:13: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:30:13: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:30:13: #1 total tags in treatment: 50972260 INFO @ Tue, 14 Jul 2020 08:30:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:30:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:30:14: #1 tags after filtering in treatment: 50972260 INFO @ Tue, 14 Jul 2020 08:30:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:30:14: #1 finished! INFO @ Tue, 14 Jul 2020 08:30:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:30:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:30:16: 46000000 INFO @ Tue, 14 Jul 2020 08:30:17: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:30:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:30:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:30:21: 47000000 INFO @ Tue, 14 Jul 2020 08:30:27: 48000000 INFO @ Tue, 14 Jul 2020 08:30:33: 49000000 INFO @ Tue, 14 Jul 2020 08:30:38: 50000000 INFO @ Tue, 14 Jul 2020 08:30:44: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:30:44: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:30:44: #1 total tags in treatment: 50972260 INFO @ Tue, 14 Jul 2020 08:30:44: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:30:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:30:45: #1 tags after filtering in treatment: 50972260 INFO @ Tue, 14 Jul 2020 08:30:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:30:45: #1 finished! INFO @ Tue, 14 Jul 2020 08:30:45: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:30:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:30:48: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:30:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:30:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088873/ERX088873.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling