Job ID = 6626445 SRX = ERX088872 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 168631220 spots for ERR111439/ERR111439.sra Written 168631220 spots for ERR111439/ERR111439.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626737 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:34 168631220 reads; of these: 168631220 (100.00%) were unpaired; of these: 6457680 (3.83%) aligned 0 times 132683656 (78.68%) aligned exactly 1 time 29489884 (17.49%) aligned >1 times 96.17% overall alignment rate Time searching: 00:44:34 Overall time: 00:44:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 106696888 / 162173540 = 0.6579 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:30: 1000000 INFO @ Tue, 14 Jul 2020 08:25:37: 2000000 INFO @ Tue, 14 Jul 2020 08:25:43: 3000000 INFO @ Tue, 14 Jul 2020 08:25:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:55: 5000000 INFO @ Tue, 14 Jul 2020 08:26:02: 6000000 INFO @ Tue, 14 Jul 2020 08:26:02: 1000000 INFO @ Tue, 14 Jul 2020 08:26:09: 7000000 INFO @ Tue, 14 Jul 2020 08:26:11: 2000000 INFO @ Tue, 14 Jul 2020 08:26:16: 8000000 INFO @ Tue, 14 Jul 2020 08:26:20: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:26:22: 9000000 INFO @ Tue, 14 Jul 2020 08:26:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:26:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:26:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:26:28: 4000000 INFO @ Tue, 14 Jul 2020 08:26:29: 10000000 INFO @ Tue, 14 Jul 2020 08:26:32: 1000000 INFO @ Tue, 14 Jul 2020 08:26:36: 11000000 INFO @ Tue, 14 Jul 2020 08:26:37: 5000000 INFO @ Tue, 14 Jul 2020 08:26:40: 2000000 INFO @ Tue, 14 Jul 2020 08:26:44: 12000000 INFO @ Tue, 14 Jul 2020 08:26:46: 6000000 INFO @ Tue, 14 Jul 2020 08:26:47: 3000000 INFO @ Tue, 14 Jul 2020 08:26:51: 13000000 INFO @ Tue, 14 Jul 2020 08:26:55: 7000000 INFO @ Tue, 14 Jul 2020 08:26:55: 4000000 INFO @ Tue, 14 Jul 2020 08:26:57: 14000000 INFO @ Tue, 14 Jul 2020 08:27:03: 5000000 INFO @ Tue, 14 Jul 2020 08:27:04: 8000000 INFO @ Tue, 14 Jul 2020 08:27:04: 15000000 INFO @ Tue, 14 Jul 2020 08:27:11: 6000000 INFO @ Tue, 14 Jul 2020 08:27:11: 16000000 INFO @ Tue, 14 Jul 2020 08:27:13: 9000000 INFO @ Tue, 14 Jul 2020 08:27:18: 17000000 INFO @ Tue, 14 Jul 2020 08:27:18: 7000000 INFO @ Tue, 14 Jul 2020 08:27:22: 10000000 INFO @ Tue, 14 Jul 2020 08:27:25: 18000000 INFO @ Tue, 14 Jul 2020 08:27:26: 8000000 INFO @ Tue, 14 Jul 2020 08:27:30: 11000000 INFO @ Tue, 14 Jul 2020 08:27:32: 19000000 INFO @ Tue, 14 Jul 2020 08:27:34: 9000000 INFO @ Tue, 14 Jul 2020 08:27:39: 12000000 INFO @ Tue, 14 Jul 2020 08:27:39: 20000000 INFO @ Tue, 14 Jul 2020 08:27:42: 10000000 INFO @ Tue, 14 Jul 2020 08:27:46: 21000000 INFO @ Tue, 14 Jul 2020 08:27:47: 13000000 INFO @ Tue, 14 Jul 2020 08:27:49: 11000000 INFO @ Tue, 14 Jul 2020 08:27:54: 22000000 INFO @ Tue, 14 Jul 2020 08:27:56: 14000000 INFO @ Tue, 14 Jul 2020 08:27:57: 12000000 INFO @ Tue, 14 Jul 2020 08:28:01: 23000000 INFO @ Tue, 14 Jul 2020 08:28:04: 13000000 INFO @ Tue, 14 Jul 2020 08:28:04: 15000000 INFO @ Tue, 14 Jul 2020 08:28:08: 24000000 INFO @ Tue, 14 Jul 2020 08:28:12: 14000000 INFO @ Tue, 14 Jul 2020 08:28:13: 16000000 INFO @ Tue, 14 Jul 2020 08:28:15: 25000000 INFO @ Tue, 14 Jul 2020 08:28:19: 15000000 INFO @ Tue, 14 Jul 2020 08:28:22: 17000000 INFO @ Tue, 14 Jul 2020 08:28:22: 26000000 INFO @ Tue, 14 Jul 2020 08:28:27: 16000000 INFO @ Tue, 14 Jul 2020 08:28:29: 27000000 INFO @ Tue, 14 Jul 2020 08:28:31: 18000000 INFO @ Tue, 14 Jul 2020 08:28:34: 17000000 INFO @ Tue, 14 Jul 2020 08:28:36: 28000000 INFO @ Tue, 14 Jul 2020 08:28:39: 19000000 INFO @ Tue, 14 Jul 2020 08:28:42: 18000000 INFO @ Tue, 14 Jul 2020 08:28:43: 29000000 INFO @ Tue, 14 Jul 2020 08:28:47: 20000000 INFO @ Tue, 14 Jul 2020 08:28:49: 19000000 INFO @ Tue, 14 Jul 2020 08:28:50: 30000000 INFO @ Tue, 14 Jul 2020 08:28:56: 21000000 INFO @ Tue, 14 Jul 2020 08:28:57: 20000000 INFO @ Tue, 14 Jul 2020 08:28:58: 31000000 INFO @ Tue, 14 Jul 2020 08:29:04: 22000000 INFO @ Tue, 14 Jul 2020 08:29:05: 21000000 INFO @ Tue, 14 Jul 2020 08:29:06: 32000000 INFO @ Tue, 14 Jul 2020 08:29:12: 22000000 INFO @ Tue, 14 Jul 2020 08:29:13: 33000000 INFO @ Tue, 14 Jul 2020 08:29:13: 23000000 INFO @ Tue, 14 Jul 2020 08:29:20: 34000000 INFO @ Tue, 14 Jul 2020 08:29:20: 23000000 INFO @ Tue, 14 Jul 2020 08:29:21: 24000000 INFO @ Tue, 14 Jul 2020 08:29:27: 35000000 INFO @ Tue, 14 Jul 2020 08:29:27: 24000000 INFO @ Tue, 14 Jul 2020 08:29:29: 25000000 INFO @ Tue, 14 Jul 2020 08:29:34: 36000000 INFO @ Tue, 14 Jul 2020 08:29:35: 25000000 INFO @ Tue, 14 Jul 2020 08:29:37: 26000000 INFO @ Tue, 14 Jul 2020 08:29:41: 37000000 INFO @ Tue, 14 Jul 2020 08:29:42: 26000000 INFO @ Tue, 14 Jul 2020 08:29:46: 27000000 INFO @ Tue, 14 Jul 2020 08:29:48: 38000000 INFO @ Tue, 14 Jul 2020 08:29:50: 27000000 INFO @ Tue, 14 Jul 2020 08:29:55: 28000000 INFO @ Tue, 14 Jul 2020 08:29:56: 39000000 INFO @ Tue, 14 Jul 2020 08:29:58: 28000000 INFO @ Tue, 14 Jul 2020 08:30:03: 29000000 INFO @ Tue, 14 Jul 2020 08:30:04: 40000000 INFO @ Tue, 14 Jul 2020 08:30:05: 29000000 INFO @ Tue, 14 Jul 2020 08:30:11: 41000000 INFO @ Tue, 14 Jul 2020 08:30:12: 30000000 INFO @ Tue, 14 Jul 2020 08:30:13: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:30:18: 42000000 INFO @ Tue, 14 Jul 2020 08:30:21: 31000000 INFO @ Tue, 14 Jul 2020 08:30:21: 31000000 INFO @ Tue, 14 Jul 2020 08:30:25: 43000000 INFO @ Tue, 14 Jul 2020 08:30:29: 32000000 INFO @ Tue, 14 Jul 2020 08:30:30: 32000000 INFO @ Tue, 14 Jul 2020 08:30:32: 44000000 INFO @ Tue, 14 Jul 2020 08:30:36: 33000000 INFO @ Tue, 14 Jul 2020 08:30:39: 33000000 INFO @ Tue, 14 Jul 2020 08:30:40: 45000000 INFO @ Tue, 14 Jul 2020 08:30:44: 34000000 INFO @ Tue, 14 Jul 2020 08:30:47: 46000000 INFO @ Tue, 14 Jul 2020 08:30:48: 34000000 INFO @ Tue, 14 Jul 2020 08:30:52: 35000000 INFO @ Tue, 14 Jul 2020 08:30:54: 47000000 INFO @ Tue, 14 Jul 2020 08:30:57: 35000000 INFO @ Tue, 14 Jul 2020 08:31:00: 36000000 INFO @ Tue, 14 Jul 2020 08:31:01: 48000000 INFO @ Tue, 14 Jul 2020 08:31:07: 36000000 INFO @ Tue, 14 Jul 2020 08:31:07: 37000000 INFO @ Tue, 14 Jul 2020 08:31:08: 49000000 INFO @ Tue, 14 Jul 2020 08:31:15: 38000000 INFO @ Tue, 14 Jul 2020 08:31:16: 37000000 INFO @ Tue, 14 Jul 2020 08:31:16: 50000000 INFO @ Tue, 14 Jul 2020 08:31:23: 39000000 INFO @ Tue, 14 Jul 2020 08:31:24: 51000000 INFO @ Tue, 14 Jul 2020 08:31:25: 38000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:31:30: 40000000 INFO @ Tue, 14 Jul 2020 08:31:32: 52000000 INFO @ Tue, 14 Jul 2020 08:31:34: 39000000 INFO @ Tue, 14 Jul 2020 08:31:38: 41000000 INFO @ Tue, 14 Jul 2020 08:31:40: 53000000 INFO @ Tue, 14 Jul 2020 08:31:42: 40000000 INFO @ Tue, 14 Jul 2020 08:31:46: 42000000 INFO @ Tue, 14 Jul 2020 08:31:48: 54000000 INFO @ Tue, 14 Jul 2020 08:31:51: 41000000 INFO @ Tue, 14 Jul 2020 08:31:54: 43000000 INFO @ Tue, 14 Jul 2020 08:31:56: 55000000 INFO @ Tue, 14 Jul 2020 08:32:00: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:32:00: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:32:00: #1 total tags in treatment: 55476652 INFO @ Tue, 14 Jul 2020 08:32:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:32:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:32:00: 42000000 INFO @ Tue, 14 Jul 2020 08:32:01: #1 tags after filtering in treatment: 55476652 INFO @ Tue, 14 Jul 2020 08:32:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:32:01: #1 finished! INFO @ Tue, 14 Jul 2020 08:32:01: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:32:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:32:01: 44000000 INFO @ Tue, 14 Jul 2020 08:32:04: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:32:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:32:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:32:08: 43000000 INFO @ Tue, 14 Jul 2020 08:32:09: 45000000 INFO @ Tue, 14 Jul 2020 08:32:16: 46000000 INFO @ Tue, 14 Jul 2020 08:32:17: 44000000 INFO @ Tue, 14 Jul 2020 08:32:24: 47000000 INFO @ Tue, 14 Jul 2020 08:32:26: 45000000 INFO @ Tue, 14 Jul 2020 08:32:32: 48000000 INFO @ Tue, 14 Jul 2020 08:32:35: 46000000 INFO @ Tue, 14 Jul 2020 08:32:40: 49000000 INFO @ Tue, 14 Jul 2020 08:32:44: 47000000 INFO @ Tue, 14 Jul 2020 08:32:47: 50000000 INFO @ Tue, 14 Jul 2020 08:32:53: 48000000 INFO @ Tue, 14 Jul 2020 08:32:55: 51000000 INFO @ Tue, 14 Jul 2020 08:33:02: 49000000 INFO @ Tue, 14 Jul 2020 08:33:03: 52000000 INFO @ Tue, 14 Jul 2020 08:33:10: 53000000 INFO @ Tue, 14 Jul 2020 08:33:11: 50000000 INFO @ Tue, 14 Jul 2020 08:33:18: 54000000 INFO @ Tue, 14 Jul 2020 08:33:20: 51000000 INFO @ Tue, 14 Jul 2020 08:33:25: 55000000 INFO @ Tue, 14 Jul 2020 08:33:29: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:33:29: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:33:29: #1 total tags in treatment: 55476652 INFO @ Tue, 14 Jul 2020 08:33:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:33:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:33:29: 52000000 INFO @ Tue, 14 Jul 2020 08:33:30: #1 tags after filtering in treatment: 55476652 INFO @ Tue, 14 Jul 2020 08:33:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:33:30: #1 finished! INFO @ Tue, 14 Jul 2020 08:33:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:33:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:33:33: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:33:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:33:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:33:38: 53000000 INFO @ Tue, 14 Jul 2020 08:33:46: 54000000 INFO @ Tue, 14 Jul 2020 08:33:54: 55000000 INFO @ Tue, 14 Jul 2020 08:33:58: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:33:58: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:33:58: #1 total tags in treatment: 55476652 INFO @ Tue, 14 Jul 2020 08:33:58: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:33:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:33:59: #1 tags after filtering in treatment: 55476652 INFO @ Tue, 14 Jul 2020 08:33:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:33:59: #1 finished! INFO @ Tue, 14 Jul 2020 08:33:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:33:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:34:02: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:34:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:34:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088872/ERX088872.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling