Job ID = 1293263 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,132,732 reads read : 28,132,732 reads written : 28,132,732 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:13 28132732 reads; of these: 28132732 (100.00%) were unpaired; of these: 790272 (2.81%) aligned 0 times 19859717 (70.59%) aligned exactly 1 time 7482743 (26.60%) aligned >1 times 97.19% overall alignment rate Time searching: 00:09:13 Overall time: 00:09:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6058474 / 27342460 = 0.2216 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 23:02:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:02:50: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:02:50: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:02:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:02:50: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:02:50: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:02:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:02:50: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:02:50: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:02:57: 1000000 INFO @ Sun, 02 Jun 2019 23:02:57: 1000000 INFO @ Sun, 02 Jun 2019 23:02:58: 1000000 INFO @ Sun, 02 Jun 2019 23:03:03: 2000000 INFO @ Sun, 02 Jun 2019 23:03:04: 2000000 INFO @ Sun, 02 Jun 2019 23:03:05: 2000000 INFO @ Sun, 02 Jun 2019 23:03:09: 3000000 INFO @ Sun, 02 Jun 2019 23:03:11: 3000000 INFO @ Sun, 02 Jun 2019 23:03:12: 3000000 INFO @ Sun, 02 Jun 2019 23:03:15: 4000000 INFO @ Sun, 02 Jun 2019 23:03:18: 4000000 INFO @ Sun, 02 Jun 2019 23:03:19: 4000000 INFO @ Sun, 02 Jun 2019 23:03:20: 5000000 INFO @ Sun, 02 Jun 2019 23:03:24: 5000000 INFO @ Sun, 02 Jun 2019 23:03:26: 5000000 INFO @ Sun, 02 Jun 2019 23:03:26: 6000000 INFO @ Sun, 02 Jun 2019 23:03:31: 6000000 INFO @ Sun, 02 Jun 2019 23:03:32: 7000000 INFO @ Sun, 02 Jun 2019 23:03:33: 6000000 INFO @ Sun, 02 Jun 2019 23:03:37: 7000000 INFO @ Sun, 02 Jun 2019 23:03:38: 8000000 INFO @ Sun, 02 Jun 2019 23:03:40: 7000000 INFO @ Sun, 02 Jun 2019 23:03:44: 8000000 INFO @ Sun, 02 Jun 2019 23:03:44: 9000000 INFO @ Sun, 02 Jun 2019 23:03:47: 8000000 INFO @ Sun, 02 Jun 2019 23:03:50: 10000000 INFO @ Sun, 02 Jun 2019 23:03:50: 9000000 INFO @ Sun, 02 Jun 2019 23:03:54: 9000000 INFO @ Sun, 02 Jun 2019 23:03:56: 11000000 INFO @ Sun, 02 Jun 2019 23:03:57: 10000000 INFO @ Sun, 02 Jun 2019 23:04:01: 10000000 INFO @ Sun, 02 Jun 2019 23:04:02: 12000000 INFO @ Sun, 02 Jun 2019 23:04:04: 11000000 INFO @ Sun, 02 Jun 2019 23:04:08: 13000000 INFO @ Sun, 02 Jun 2019 23:04:08: 11000000 INFO @ Sun, 02 Jun 2019 23:04:10: 12000000 INFO @ Sun, 02 Jun 2019 23:04:14: 14000000 INFO @ Sun, 02 Jun 2019 23:04:15: 12000000 INFO @ Sun, 02 Jun 2019 23:04:17: 13000000 INFO @ Sun, 02 Jun 2019 23:04:20: 15000000 INFO @ Sun, 02 Jun 2019 23:04:22: 13000000 INFO @ Sun, 02 Jun 2019 23:04:23: 14000000 INFO @ Sun, 02 Jun 2019 23:04:26: 16000000 INFO @ Sun, 02 Jun 2019 23:04:29: 14000000 INFO @ Sun, 02 Jun 2019 23:04:30: 15000000 INFO @ Sun, 02 Jun 2019 23:04:32: 17000000 INFO @ Sun, 02 Jun 2019 23:04:37: 15000000 INFO @ Sun, 02 Jun 2019 23:04:37: 16000000 INFO @ Sun, 02 Jun 2019 23:04:38: 18000000 INFO @ Sun, 02 Jun 2019 23:04:43: 17000000 INFO @ Sun, 02 Jun 2019 23:04:44: 16000000 INFO @ Sun, 02 Jun 2019 23:04:44: 19000000 INFO @ Sun, 02 Jun 2019 23:04:50: 20000000 INFO @ Sun, 02 Jun 2019 23:04:50: 18000000 INFO @ Sun, 02 Jun 2019 23:04:51: 17000000 INFO @ Sun, 02 Jun 2019 23:04:56: 21000000 INFO @ Sun, 02 Jun 2019 23:04:56: 19000000 INFO @ Sun, 02 Jun 2019 23:04:57: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:04:57: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:04:57: #1 total tags in treatment: 21283986 INFO @ Sun, 02 Jun 2019 23:04:57: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:04:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:04:58: 18000000 INFO @ Sun, 02 Jun 2019 23:04:58: #1 tags after filtering in treatment: 21283986 INFO @ Sun, 02 Jun 2019 23:04:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:04:58: #1 finished! INFO @ Sun, 02 Jun 2019 23:04:58: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:04:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:05:00: #2 number of paired peaks: 35 WARNING @ Sun, 02 Jun 2019 23:05:00: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:05:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:05:02: 20000000 INFO @ Sun, 02 Jun 2019 23:05:05: 19000000 INFO @ Sun, 02 Jun 2019 23:05:10: 21000000 INFO @ Sun, 02 Jun 2019 23:05:11: 20000000 INFO @ Sun, 02 Jun 2019 23:05:12: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:05:12: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:05:12: #1 total tags in treatment: 21283986 INFO @ Sun, 02 Jun 2019 23:05:12: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:05:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:05:12: #1 tags after filtering in treatment: 21283986 INFO @ Sun, 02 Jun 2019 23:05:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:05:12: #1 finished! INFO @ Sun, 02 Jun 2019 23:05:12: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:05:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:05:14: #2 number of paired peaks: 35 WARNING @ Sun, 02 Jun 2019 23:05:14: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:05:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:05:18: 21000000 INFO @ Sun, 02 Jun 2019 23:05:20: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:05:20: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:05:20: #1 total tags in treatment: 21283986 INFO @ Sun, 02 Jun 2019 23:05:20: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:05:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:05:21: #1 tags after filtering in treatment: 21283986 INFO @ Sun, 02 Jun 2019 23:05:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:05:21: #1 finished! INFO @ Sun, 02 Jun 2019 23:05:21: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:05:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:05:23: #2 number of paired peaks: 35 WARNING @ Sun, 02 Jun 2019 23:05:23: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:05:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX011405/ERX011405.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。