Job ID = 1293262 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,771,193 reads read : 3,771,193 reads written : 3,771,193 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:39 3771193 reads; of these: 3771193 (100.00%) were unpaired; of these: 7649 (0.20%) aligned 0 times 3392185 (89.95%) aligned exactly 1 time 371359 (9.85%) aligned >1 times 99.80% overall alignment rate Time searching: 00:00:39 Overall time: 00:00:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 207153 / 3763544 = 0.0550 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:34:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:34:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:34:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:34:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:34:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:34:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:34:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:34:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:34:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:34:23: 1000000 INFO @ Sun, 02 Jun 2019 22:34:24: 1000000 INFO @ Sun, 02 Jun 2019 22:34:24: 1000000 INFO @ Sun, 02 Jun 2019 22:34:29: 2000000 INFO @ Sun, 02 Jun 2019 22:34:31: 2000000 INFO @ Sun, 02 Jun 2019 22:34:31: 2000000 INFO @ Sun, 02 Jun 2019 22:34:36: 3000000 INFO @ Sun, 02 Jun 2019 22:34:38: 3000000 INFO @ Sun, 02 Jun 2019 22:34:38: 3000000 INFO @ Sun, 02 Jun 2019 22:34:39: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:34:39: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:34:39: #1 total tags in treatment: 3556391 INFO @ Sun, 02 Jun 2019 22:34:39: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:34:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:34:39: #1 tags after filtering in treatment: 3556391 INFO @ Sun, 02 Jun 2019 22:34:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:34:39: #1 finished! INFO @ Sun, 02 Jun 2019 22:34:39: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:34:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:34:40: #2 number of paired peaks: 39 WARNING @ Sun, 02 Jun 2019 22:34:40: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:34:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:34:41: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:34:41: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:34:41: #1 total tags in treatment: 3556391 INFO @ Sun, 02 Jun 2019 22:34:41: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:34:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:34:41: #1 tags after filtering in treatment: 3556391 INFO @ Sun, 02 Jun 2019 22:34:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:34:41: #1 finished! INFO @ Sun, 02 Jun 2019 22:34:41: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:34:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:34:42: #2 number of paired peaks: 39 WARNING @ Sun, 02 Jun 2019 22:34:42: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:34:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:34:42: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:34:42: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:34:42: #1 total tags in treatment: 3556391 INFO @ Sun, 02 Jun 2019 22:34:42: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:34:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:34:42: #1 tags after filtering in treatment: 3556391 INFO @ Sun, 02 Jun 2019 22:34:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:34:42: #1 finished! INFO @ Sun, 02 Jun 2019 22:34:42: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:34:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:34:42: #2 number of paired peaks: 39 WARNING @ Sun, 02 Jun 2019 22:34:42: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:34:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008197/ERX008197.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。