Job ID = 1293256 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,809,280 reads read : 8,809,280 reads written : 8,809,280 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:23 8809280 reads; of these: 8809280 (100.00%) were unpaired; of these: 31276 (0.36%) aligned 0 times 8581451 (97.41%) aligned exactly 1 time 196553 (2.23%) aligned >1 times 99.64% overall alignment rate Time searching: 00:01:23 Overall time: 00:01:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 362805 / 8778004 = 0.0413 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:37:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:37:34: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:37:34: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:37:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:37:34: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:37:34: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:37:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:37:34: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:37:34: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:37:42: 1000000 INFO @ Sun, 02 Jun 2019 22:37:43: 1000000 INFO @ Sun, 02 Jun 2019 22:37:43: 1000000 INFO @ Sun, 02 Jun 2019 22:37:49: 2000000 INFO @ Sun, 02 Jun 2019 22:37:52: 2000000 INFO @ Sun, 02 Jun 2019 22:37:52: 2000000 INFO @ Sun, 02 Jun 2019 22:37:58: 3000000 INFO @ Sun, 02 Jun 2019 22:38:01: 3000000 INFO @ Sun, 02 Jun 2019 22:38:01: 3000000 INFO @ Sun, 02 Jun 2019 22:38:06: 4000000 INFO @ Sun, 02 Jun 2019 22:38:11: 4000000 INFO @ Sun, 02 Jun 2019 22:38:11: 4000000 INFO @ Sun, 02 Jun 2019 22:38:13: 5000000 INFO @ Sun, 02 Jun 2019 22:38:21: 5000000 INFO @ Sun, 02 Jun 2019 22:38:21: 5000000 INFO @ Sun, 02 Jun 2019 22:38:21: 6000000 INFO @ Sun, 02 Jun 2019 22:38:28: 7000000 INFO @ Sun, 02 Jun 2019 22:38:30: 6000000 INFO @ Sun, 02 Jun 2019 22:38:30: 6000000 INFO @ Sun, 02 Jun 2019 22:38:36: 8000000 INFO @ Sun, 02 Jun 2019 22:38:39: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:38:39: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:38:39: #1 total tags in treatment: 8415199 INFO @ Sun, 02 Jun 2019 22:38:39: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:38:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:38:39: #1 tags after filtering in treatment: 8415199 INFO @ Sun, 02 Jun 2019 22:38:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:38:39: #1 finished! INFO @ Sun, 02 Jun 2019 22:38:39: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:38:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:38:39: 7000000 INFO @ Sun, 02 Jun 2019 22:38:39: 7000000 INFO @ Sun, 02 Jun 2019 22:38:40: #2 number of paired peaks: 35 WARNING @ Sun, 02 Jun 2019 22:38:40: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:38:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:38:48: 8000000 INFO @ Sun, 02 Jun 2019 22:38:48: 8000000 INFO @ Sun, 02 Jun 2019 22:38:52: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:38:52: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:38:52: #1 total tags in treatment: 8415199 INFO @ Sun, 02 Jun 2019 22:38:52: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:38:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:38:52: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:38:52: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:38:52: #1 total tags in treatment: 8415199 INFO @ Sun, 02 Jun 2019 22:38:52: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:38:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:38:52: #1 tags after filtering in treatment: 8415199 INFO @ Sun, 02 Jun 2019 22:38:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:38:52: #1 finished! INFO @ Sun, 02 Jun 2019 22:38:52: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:38:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:38:52: #1 tags after filtering in treatment: 8415199 INFO @ Sun, 02 Jun 2019 22:38:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:38:52: #1 finished! INFO @ Sun, 02 Jun 2019 22:38:52: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:38:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:38:53: #2 number of paired peaks: 35 WARNING @ Sun, 02 Jun 2019 22:38:53: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:38:53: Process for pairing-model is terminated! INFO @ Sun, 02 Jun 2019 22:38:53: #2 number of paired peaks: 35 WARNING @ Sun, 02 Jun 2019 22:38:53: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:38:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis cut: /home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.20_peaks.narrowPeak: No such file or directory needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008180/ERX008180.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。