SRX234919 SAMN01919564 GSM1076686:_Snyder_NHR-116_GFP_L4_rep2_Input_TGCT ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 6800798 Illumina_Genome_Analyzer SRA066562 SRS393225 SRP018560 - 2015-07-22T21:03:38Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_NHR-116_GFP_L4_rep2_Input_TGCT strain=OP226(official name : OP226 genotype : unc119(ed3); wgIs226(nhr-116::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-116 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-116::EGFP fusion protein is expressed in the correct nhr-116 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-116 transcription factor. made_by : M Sarov ) developmental stage=L4 genotype=unc119(ed3);wgIs226(nhr-116::TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=nhr-116