SRX234918 SAMN01919563 GSM1076685:_Snyder_NHR-116_GFP_L4_rep1_Input_ACGT ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 6696139 Illumina_Genome_Analyzer SRA066562 SRS393224 SRP018560 - 2015-07-22T21:03:38Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_NHR-116_GFP_L4_rep1_Input_ACGT strain=OP226(official name : OP226 genotype : unc119(ed3); wgIs226(nhr-116::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-116 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-116::EGFP fusion protein is expressed in the correct nhr-116 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-116 transcription factor. made_by : M Sarov ) developmental stage=L4 genotype=unc119(ed3);wgIs226(nhr-116::TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=nhr-116