SRX233457 SAMN01919374 GSM1076683:_Snyder_NHR-77_GFP_L1_rep1_GFP_ACGT ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 9931760 Illumina_Genome_Analyzer SRA066483 SRS393123 SRP018544 - 2015-07-22T21:03:26Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_NHR-77_GFP_L1_rep1_GFP_ACGT strain=OP353(official name : OP353 genotype : unc119(ed3); wgIs353(nhr-77::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-77 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-77::EGFP fusion protein is expressed in the correct nhr-77 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-77 transcription factor. made_by : R Waterston ) developmental stage=fed L1 genotype=unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=nhr-77