SRX233456 SAMN01919373 GSM1076682:_Snyder_NHR-77_GFP_L1_rep2_Input_CATT ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5538997 Illumina_Genome_Analyzer SRA066483 SRS393122 SRP018544 - 2015-07-22T21:03:26Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_NHR-77_GFP_L1_rep2_Input_CATT strain=OP353(official name : OP353 genotype : unc119(ed3); wgIs353(nhr-77::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-77 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-77::EGFP fusion protein is expressed in the correct nhr-77 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-77 transcription factor. made_by : R Waterston ) developmental stage=fed L1 genotype=unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=nhr-77