SRX147641 SAMN00993163 GSM929328:_Snyder_NHR-116_GFP_L2_rep1_extraction1_seq1_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 7461455 Illumina_Genome_Analyzer SRA052694 SRS332892 SRP013014 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_NHR-116_GFP_L2_rep1 strain=OP226(official name : OP226 genotype : unc119(ed3); wgIs226(nhr-116::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-116 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-116::EGFP fusion protein is expressed in the correct nhr-116 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-116 transcription factor. made_by : M Sarov ) developmental stage=L2 genotype=unc119(ed3);wgIs226(nhr-116::TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=nhr-116