SRX147630 SAMN00993152 GSM929317:_Snyder_NHR-77_GFP_L2_rep2_extraction2_seq1_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 6036969 Illumina_Genome_Analyzer SRA052691 SRS332881 SRP013011 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_NHR-77_GFP_L2_rep2 strain=OP353(official name : OP353 genotype : unc119(ed3); wgIs353(nhr-77::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-77 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-77::EGFP fusion protein is expressed in the correct nhr-77 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-77 transcription factor. made_by : R Waterston ) developmental stage=L2 genotype=unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=nhr-77