SRX146512 SAMN00991467 GSM928358:_Snyder_DAF12_GFP_L3_rep1_extraction1_seq1_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 4723194 Illumina_Genome_Analyzer SRA052587 SRS332087 SRP012917 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_DAF12_GFP_L3_rep1 strain=OP167(official name : OP167 genotype : unc119(ed3); wgIs167(daf-12::TY1 EGFP FLAG; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : S Kim ) developmental stage=L3 genotype=unc119(ed3);wgIs167(daf-12::TY1 EGFP FLAG;unc119) Sex=hermaphrodite