SRX065715 SAMN00620893 GSM729376:_Snyder_ALY-2_Input_L3_extraction1_seq1_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5348774 Illumina_Genome_Analyzer SRA037226 SRS209126 SRP006869 PRJNA141635 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_ALY-2_Input_L3 extraction1_seq1 channel_1 strain=OP217(official name : OP217 genotype : unc119(ed3); wgIs217(aly-2:TY1 EGFP FLAG; unc119) outcross : 3 transgene : aly-2 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ALY-2::EGFP fusion protein is expressed in the correct aly-2 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ALY-2 transcription factor. made_by : Mihail Sarov ) developmental stage=L3 genotype=unc119(ed3);wgIs217(aly-2:TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=aly-2