SRX065713 SAMN00620891 GSM729370:_Snyder_DAF-12_GFP_L4_rep1_extraction3_seq3_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5788678 Illumina_Genome_Analyzer SRA037225 SRS209124 SRP006868 PRJNA141631 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_DAF-12_GFP_L4_rep1 extraction3_seq3 channel_1 strain=OP222(official name : OP222 genotype : unc119(ed3); wgIs222(daf-12:TY1 EGFP FLAG; unc119) outcross : 3 transgene : daf-12 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : Mihail Sarov ) developmental stage=L4 genotype=unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=daf-12