SRX065709 SAMN00620887 GSM729382:_Snyder_ALY-2_GFP_L1_rep1_extraction3_seq3_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5679829 Illumina_Genome_Analyzer SRA037224 SRS209120 SRP006867 PRJNA141637 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_ALY-2_GFP_L1_rep1 extraction3_seq3 channel_1 strain=OP217(official name : OP217 genotype : unc119(ed3); wgIs217(aly-2:TY1 EGFP FLAG; unc119) outcross : 3 transgene : aly-2 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ALY-2::EGFP fusion protein is expressed in the correct aly-2 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ALY-2 transcription factor. made_by : Mihail Sarov ) developmental stage=fed L1 genotype=unc119(ed3);wgIs217(aly-2:TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=aly-2