SRX065699 SAMN00620877 GSM729364:_Snyder_F45C12.2_Input_L3_extraction1_seq1_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 4169447 Illumina_Genome_Analyzer SRA037222 SRS209110 SRP006865 PRJNA141629 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_F45C12.2_Input_L3 extraction1_seq1 channel_1 strain=OP212(official name : OP212 genotype : unc119(ed3); wgIs212(F45C12.2:TY1 EGFP FLAG; unc119) outcross : 3 transgene : F45C12.2 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The F45C12.2::EGFP fusion protein is expressed in the correct F45C12.2 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the F45C12.2 transcription factor. made_by : Mihail Sarov ) developmental stage=L3 genotype=unc119(ed3);wgIs212(F45C12.2:TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=F45C12.2