SRX065697 SAMN00620875 GSM729362:_Snyder_ZAG-1_GFP_L1_rep1_extraction3_seq3_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 3661211 Illumina_Genome_Analyzer SRA037221 SRS209108 SRP006864 PRJNA141627 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_ZAG-1_GFP_L1_rep1 extraction3_seq3 channel_1 strain=OP83(official name : OP83 genotype : unc119(ed3); wgIs83(zag-1::TY1 EGFP FLAG; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ZAG-1::EGFP fusion protein is expressed in the correct zag-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ZAG-1 transcription factor. made_by : R Waterston ) developmental stage=fed L1 genotype=unc119(ed3);wgIs83(zag-1::TY1 EGFP FLAG;unc119) Sex=hermaphrodite