SRX054307 SAMN00254102 GSM700186:_Snyder_EGL-5_GFP_L3_rep2_extraction5_seq5_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5195407 Illumina_Genome_Analyzer SRA030939 SRS183358 SRP006201 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_EGL-5_GFP_L3_rep2 extraction5_seq5 channel_1 strain=OP54(official name : OP54 genotype : unc-119(ed3) III; wgIs54 [unc-119(+) egl-5::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The EGL-5::EGFP fusion protein is expressed in the correct egl-5 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the EGL-5 transcription factor. made_by : R. Waterston and S. Kim ) developmental stage=L3 genotype=unc-119(ed3) III; wgIs54 [unc-119(+) egl-5::TY1::EGFP::3xFLAG] Sex=hermaphrodite