SRX054254 SAMN00254085 GSM700178:_Snyder_PHA-4_GFP_L2_rep2_extraction2_seq2_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5858168 Illumina_Genome_Analyzer SRA030937 SRS183341 SRP006199 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_PHA-4_GFP_L2_rep2 extraction2_seq2 channel_1 strain=OP37(official name : OP37 genotype : unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PHA-4::EGFP fusion protein is expressed in the correct pha-4 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PHA-4 transcription factor. made_by : R. Waterston and S. Kim ) developmental stage=L2 genotype=unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] Sex=hermaphrodite