SRX054208 SAMN00254075 GSM700194:_Snyder_PHA-4_GFP_YA_rep2_extraction4_seq4_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 857311 Illumina_Genome_Analyzer SRA030935 SRS183331 SRP006197 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_PHA-4_GFP_YA_rep2 extraction4_seq4 channel_1 strain=OP37(official name : OP37 genotype : unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PHA-4::EGFP fusion protein is expressed in the correct pha-4 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PHA-4 transcription factor. made_by : R. Waterston and S. Kim ) developmental stage=Young Adult genotype=unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] Sex=hermaphrodite