SRX043975 SAMN00216240 GSM677655:_Snyder_PHA4_GFP_lemb_rep1_extraction1_seq1_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 4302276 Illumina_Genome_Analyzer SRA030386 SRS173162 SRP005879 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_PHA4_GFP_lemb_rep1 extraction1_seq1 channel_1 strain=OP37(made_by : R. Waterston and S. Kim description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PHA-4::EGFP fusion protein is expressed in the correct pha-4 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PHA-4 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] official name : OP37 ) developmental stage=late embryo genotype=unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] Sex=hermaphrodite