Job ID = 14159142 SRX = SRX9120610 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42619141 spots for SRR12638553/SRR12638553.sra Written 42619141 spots for SRR12638553/SRR12638553.sra fastq に変換しました。 bowtie でマッピング中... Your job 14159415 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:41 42619141 reads; of these: 42619141 (100.00%) were unpaired; of these: 4682240 (10.99%) aligned 0 times 31268135 (73.37%) aligned exactly 1 time 6668766 (15.65%) aligned >1 times 89.01% overall alignment rate Time searching: 00:09:42 Overall time: 00:09:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7557388 / 37936901 = 0.1992 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 20:15:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 20:15:53: #1 read tag files... INFO @ Wed, 08 Dec 2021 20:15:53: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 20:16:03: 1000000 INFO @ Wed, 08 Dec 2021 20:16:13: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 20:16:22: 3000000 INFO @ Wed, 08 Dec 2021 20:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 20:16:23: #1 read tag files... INFO @ Wed, 08 Dec 2021 20:16:23: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 20:16:31: 1000000 INFO @ Wed, 08 Dec 2021 20:16:31: 4000000 INFO @ Wed, 08 Dec 2021 20:16:38: 2000000 INFO @ Wed, 08 Dec 2021 20:16:41: 5000000 INFO @ Wed, 08 Dec 2021 20:16:46: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 20:16:51: 6000000 INFO @ Wed, 08 Dec 2021 20:16:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 20:16:53: #1 read tag files... INFO @ Wed, 08 Dec 2021 20:16:53: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 20:16:55: 4000000 INFO @ Wed, 08 Dec 2021 20:17:01: 7000000 INFO @ Wed, 08 Dec 2021 20:17:03: 1000000 INFO @ Wed, 08 Dec 2021 20:17:03: 5000000 INFO @ Wed, 08 Dec 2021 20:17:11: 8000000 INFO @ Wed, 08 Dec 2021 20:17:11: 6000000 INFO @ Wed, 08 Dec 2021 20:17:11: 2000000 INFO @ Wed, 08 Dec 2021 20:17:19: 3000000 INFO @ Wed, 08 Dec 2021 20:17:19: 7000000 INFO @ Wed, 08 Dec 2021 20:17:21: 9000000 INFO @ Wed, 08 Dec 2021 20:17:28: 4000000 INFO @ Wed, 08 Dec 2021 20:17:28: 8000000 INFO @ Wed, 08 Dec 2021 20:17:31: 10000000 INFO @ Wed, 08 Dec 2021 20:17:37: 5000000 INFO @ Wed, 08 Dec 2021 20:17:37: 9000000 INFO @ Wed, 08 Dec 2021 20:17:41: 11000000 INFO @ Wed, 08 Dec 2021 20:17:46: 6000000 INFO @ Wed, 08 Dec 2021 20:17:46: 10000000 INFO @ Wed, 08 Dec 2021 20:17:51: 12000000 INFO @ Wed, 08 Dec 2021 20:17:56: 7000000 INFO @ Wed, 08 Dec 2021 20:17:56: 11000000 INFO @ Wed, 08 Dec 2021 20:18:02: 13000000 INFO @ Wed, 08 Dec 2021 20:18:05: 12000000 INFO @ Wed, 08 Dec 2021 20:18:06: 8000000 INFO @ Wed, 08 Dec 2021 20:18:13: 14000000 INFO @ Wed, 08 Dec 2021 20:18:14: 13000000 INFO @ Wed, 08 Dec 2021 20:18:15: 9000000 INFO @ Wed, 08 Dec 2021 20:18:23: 15000000 INFO @ Wed, 08 Dec 2021 20:18:24: 14000000 INFO @ Wed, 08 Dec 2021 20:18:24: 10000000 INFO @ Wed, 08 Dec 2021 20:18:33: 15000000 INFO @ Wed, 08 Dec 2021 20:18:33: 11000000 INFO @ Wed, 08 Dec 2021 20:18:34: 16000000 INFO @ Wed, 08 Dec 2021 20:18:43: 16000000 INFO @ Wed, 08 Dec 2021 20:18:43: 12000000 INFO @ Wed, 08 Dec 2021 20:18:44: 17000000 INFO @ Wed, 08 Dec 2021 20:18:51: 17000000 INFO @ Wed, 08 Dec 2021 20:18:52: 13000000 INFO @ Wed, 08 Dec 2021 20:18:55: 18000000 INFO @ Wed, 08 Dec 2021 20:18:58: 18000000 INFO @ Wed, 08 Dec 2021 20:19:00: 14000000 INFO @ Wed, 08 Dec 2021 20:19:06: 19000000 INFO @ Wed, 08 Dec 2021 20:19:06: 19000000 INFO @ Wed, 08 Dec 2021 20:19:09: 15000000 INFO @ Wed, 08 Dec 2021 20:19:15: 20000000 INFO @ Wed, 08 Dec 2021 20:19:17: 20000000 INFO @ Wed, 08 Dec 2021 20:19:17: 16000000 INFO @ Wed, 08 Dec 2021 20:19:23: 21000000 INFO @ Wed, 08 Dec 2021 20:19:25: 17000000 INFO @ Wed, 08 Dec 2021 20:19:27: 21000000 INFO @ Wed, 08 Dec 2021 20:19:32: 22000000 INFO @ Wed, 08 Dec 2021 20:19:34: 18000000 INFO @ Wed, 08 Dec 2021 20:19:37: 22000000 INFO @ Wed, 08 Dec 2021 20:19:42: 23000000 INFO @ Wed, 08 Dec 2021 20:19:43: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 20:19:48: 23000000 INFO @ Wed, 08 Dec 2021 20:19:51: 24000000 INFO @ Wed, 08 Dec 2021 20:19:52: 20000000 INFO @ Wed, 08 Dec 2021 20:19:59: 24000000 INFO @ Wed, 08 Dec 2021 20:19:59: 25000000 INFO @ Wed, 08 Dec 2021 20:20:01: 21000000 INFO @ Wed, 08 Dec 2021 20:20:07: 26000000 INFO @ Wed, 08 Dec 2021 20:20:09: 25000000 INFO @ Wed, 08 Dec 2021 20:20:10: 22000000 INFO @ Wed, 08 Dec 2021 20:20:15: 27000000 INFO @ Wed, 08 Dec 2021 20:20:18: 23000000 INFO @ Wed, 08 Dec 2021 20:20:20: 26000000 INFO @ Wed, 08 Dec 2021 20:20:24: 28000000 INFO @ Wed, 08 Dec 2021 20:20:27: 24000000 INFO @ Wed, 08 Dec 2021 20:20:30: 27000000 INFO @ Wed, 08 Dec 2021 20:20:32: 29000000 INFO @ Wed, 08 Dec 2021 20:20:36: 25000000 INFO @ Wed, 08 Dec 2021 20:20:41: 30000000 INFO @ Wed, 08 Dec 2021 20:20:41: 28000000 INFO @ Wed, 08 Dec 2021 20:20:44: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 20:20:44: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 20:20:44: #1 total tags in treatment: 30379513 INFO @ Wed, 08 Dec 2021 20:20:44: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 20:20:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 20:20:44: #1 tags after filtering in treatment: 30379513 INFO @ Wed, 08 Dec 2021 20:20:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 20:20:44: #1 finished! INFO @ Wed, 08 Dec 2021 20:20:44: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 20:20:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 20:20:44: 26000000 INFO @ Wed, 08 Dec 2021 20:20:47: #2 number of paired peaks: 98 WARNING @ Wed, 08 Dec 2021 20:20:47: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 20:20:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 20:20:51: 29000000 INFO @ Wed, 08 Dec 2021 20:20:53: 27000000 INFO @ Wed, 08 Dec 2021 20:21:01: 28000000 INFO @ Wed, 08 Dec 2021 20:21:01: 30000000 INFO @ Wed, 08 Dec 2021 20:21:05: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 20:21:05: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 20:21:05: #1 total tags in treatment: 30379513 INFO @ Wed, 08 Dec 2021 20:21:05: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 20:21:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 20:21:06: #1 tags after filtering in treatment: 30379513 INFO @ Wed, 08 Dec 2021 20:21:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 20:21:06: #1 finished! INFO @ Wed, 08 Dec 2021 20:21:06: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 20:21:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 20:21:08: #2 number of paired peaks: 98 WARNING @ Wed, 08 Dec 2021 20:21:08: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 20:21:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_peaks.narrowPeak: No such file or directory INFO @ Wed, 08 Dec 2021 20:21:08: 29000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 20:21:16: 30000000 INFO @ Wed, 08 Dec 2021 20:21:20: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 20:21:20: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 20:21:20: #1 total tags in treatment: 30379513 INFO @ Wed, 08 Dec 2021 20:21:20: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 20:21:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 20:21:20: #1 tags after filtering in treatment: 30379513 INFO @ Wed, 08 Dec 2021 20:21:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 20:21:20: #1 finished! INFO @ Wed, 08 Dec 2021 20:21:20: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 20:21:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 20:21:23: #2 number of paired peaks: 98 WARNING @ Wed, 08 Dec 2021 20:21:23: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 20:21:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling