Job ID = 14157918 SRX = SRX8948260 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 49692684 spots for SRR12453804/SRR12453804.sra Written 49692684 spots for SRR12453804/SRR12453804.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158229 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:46:39 49692684 reads; of these: 49692684 (100.00%) were paired; of these: 19643032 (39.53%) aligned concordantly 0 times 25746933 (51.81%) aligned concordantly exactly 1 time 4302719 (8.66%) aligned concordantly >1 times ---- 19643032 pairs aligned concordantly 0 times; of these: 347229 (1.77%) aligned discordantly 1 time ---- 19295803 pairs aligned 0 times concordantly or discordantly; of these: 38591606 mates make up the pairs; of these: 38227396 (99.06%) aligned 0 times 218025 (0.56%) aligned exactly 1 time 146185 (0.38%) aligned >1 times 61.54% overall alignment rate Time searching: 00:46:39 Overall time: 00:46:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2451434 / 30047915 = 0.0816 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:59:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:59:34: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:59:34: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:59:42: 1000000 INFO @ Wed, 08 Dec 2021 13:59:50: 2000000 INFO @ Wed, 08 Dec 2021 13:59:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:00:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:00:03: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:00:03: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:00:06: 4000000 INFO @ Wed, 08 Dec 2021 14:00:15: 5000000 INFO @ Wed, 08 Dec 2021 14:00:15: 1000000 INFO @ Wed, 08 Dec 2021 14:00:23: 6000000 INFO @ Wed, 08 Dec 2021 14:00:26: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:00:32: 7000000 INFO @ Wed, 08 Dec 2021 14:00:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:00:33: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:00:33: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:00:38: 3000000 INFO @ Wed, 08 Dec 2021 14:00:41: 8000000 INFO @ Wed, 08 Dec 2021 14:00:46: 1000000 INFO @ Wed, 08 Dec 2021 14:00:50: 9000000 INFO @ Wed, 08 Dec 2021 14:00:50: 4000000 INFO @ Wed, 08 Dec 2021 14:00:58: 10000000 INFO @ Wed, 08 Dec 2021 14:00:59: 2000000 INFO @ Wed, 08 Dec 2021 14:01:02: 5000000 INFO @ Wed, 08 Dec 2021 14:01:06: 11000000 INFO @ Wed, 08 Dec 2021 14:01:13: 3000000 INFO @ Wed, 08 Dec 2021 14:01:14: 12000000 INFO @ Wed, 08 Dec 2021 14:01:15: 6000000 INFO @ Wed, 08 Dec 2021 14:01:22: 13000000 INFO @ Wed, 08 Dec 2021 14:01:26: 4000000 INFO @ Wed, 08 Dec 2021 14:01:28: 7000000 INFO @ Wed, 08 Dec 2021 14:01:29: 14000000 INFO @ Wed, 08 Dec 2021 14:01:38: 15000000 INFO @ Wed, 08 Dec 2021 14:01:38: 5000000 INFO @ Wed, 08 Dec 2021 14:01:41: 8000000 INFO @ Wed, 08 Dec 2021 14:01:47: 16000000 INFO @ Wed, 08 Dec 2021 14:01:51: 6000000 INFO @ Wed, 08 Dec 2021 14:01:53: 9000000 INFO @ Wed, 08 Dec 2021 14:01:55: 17000000 INFO @ Wed, 08 Dec 2021 14:02:04: 7000000 INFO @ Wed, 08 Dec 2021 14:02:04: 18000000 INFO @ Wed, 08 Dec 2021 14:02:06: 10000000 INFO @ Wed, 08 Dec 2021 14:02:13: 19000000 INFO @ Wed, 08 Dec 2021 14:02:16: 8000000 INFO @ Wed, 08 Dec 2021 14:02:18: 11000000 INFO @ Wed, 08 Dec 2021 14:02:21: 20000000 INFO @ Wed, 08 Dec 2021 14:02:29: 9000000 INFO @ Wed, 08 Dec 2021 14:02:29: 21000000 INFO @ Wed, 08 Dec 2021 14:02:30: 12000000 INFO @ Wed, 08 Dec 2021 14:02:37: 22000000 INFO @ Wed, 08 Dec 2021 14:02:41: 10000000 INFO @ Wed, 08 Dec 2021 14:02:42: 13000000 INFO @ Wed, 08 Dec 2021 14:02:45: 23000000 INFO @ Wed, 08 Dec 2021 14:02:53: 11000000 INFO @ Wed, 08 Dec 2021 14:02:54: 14000000 INFO @ Wed, 08 Dec 2021 14:02:55: 24000000 INFO @ Wed, 08 Dec 2021 14:03:03: 25000000 INFO @ Wed, 08 Dec 2021 14:03:05: 12000000 INFO @ Wed, 08 Dec 2021 14:03:06: 15000000 INFO @ Wed, 08 Dec 2021 14:03:11: 26000000 INFO @ Wed, 08 Dec 2021 14:03:17: 13000000 INFO @ Wed, 08 Dec 2021 14:03:18: 16000000 INFO @ Wed, 08 Dec 2021 14:03:19: 27000000 INFO @ Wed, 08 Dec 2021 14:03:27: 28000000 INFO @ Wed, 08 Dec 2021 14:03:30: 14000000 INFO @ Wed, 08 Dec 2021 14:03:31: 17000000 INFO @ Wed, 08 Dec 2021 14:03:35: 29000000 INFO @ Wed, 08 Dec 2021 14:03:42: 15000000 INFO @ Wed, 08 Dec 2021 14:03:43: 30000000 INFO @ Wed, 08 Dec 2021 14:03:43: 18000000 INFO @ Wed, 08 Dec 2021 14:03:50: 31000000 INFO @ Wed, 08 Dec 2021 14:03:54: 16000000 INFO @ Wed, 08 Dec 2021 14:03:56: 19000000 INFO @ Wed, 08 Dec 2021 14:03:58: 32000000 INFO @ Wed, 08 Dec 2021 14:04:06: 33000000 INFO @ Wed, 08 Dec 2021 14:04:07: 17000000 INFO @ Wed, 08 Dec 2021 14:04:08: 20000000 INFO @ Wed, 08 Dec 2021 14:04:14: 34000000 INFO @ Wed, 08 Dec 2021 14:04:19: 18000000 INFO @ Wed, 08 Dec 2021 14:04:20: 21000000 INFO @ Wed, 08 Dec 2021 14:04:22: 35000000 INFO @ Wed, 08 Dec 2021 14:04:31: 36000000 INFO @ Wed, 08 Dec 2021 14:04:31: 19000000 INFO @ Wed, 08 Dec 2021 14:04:32: 22000000 INFO @ Wed, 08 Dec 2021 14:04:39: 37000000 INFO @ Wed, 08 Dec 2021 14:04:43: 20000000 INFO @ Wed, 08 Dec 2021 14:04:44: 23000000 INFO @ Wed, 08 Dec 2021 14:04:48: 38000000 INFO @ Wed, 08 Dec 2021 14:04:55: 24000000 INFO @ Wed, 08 Dec 2021 14:04:55: 21000000 INFO @ Wed, 08 Dec 2021 14:04:56: 39000000 INFO @ Wed, 08 Dec 2021 14:05:05: 40000000 INFO @ Wed, 08 Dec 2021 14:05:08: 25000000 INFO @ Wed, 08 Dec 2021 14:05:08: 22000000 INFO @ Wed, 08 Dec 2021 14:05:13: 41000000 INFO @ Wed, 08 Dec 2021 14:05:20: 26000000 INFO @ Wed, 08 Dec 2021 14:05:20: 23000000 INFO @ Wed, 08 Dec 2021 14:05:21: 42000000 INFO @ Wed, 08 Dec 2021 14:05:29: 43000000 INFO @ Wed, 08 Dec 2021 14:05:32: 27000000 INFO @ Wed, 08 Dec 2021 14:05:32: 24000000 INFO @ Wed, 08 Dec 2021 14:05:37: 44000000 INFO @ Wed, 08 Dec 2021 14:05:44: 28000000 INFO @ Wed, 08 Dec 2021 14:05:44: 25000000 INFO @ Wed, 08 Dec 2021 14:05:45: 45000000 INFO @ Wed, 08 Dec 2021 14:05:53: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:05:56: 29000000 INFO @ Wed, 08 Dec 2021 14:05:57: 26000000 INFO @ Wed, 08 Dec 2021 14:06:01: 47000000 INFO @ Wed, 08 Dec 2021 14:06:08: 30000000 INFO @ Wed, 08 Dec 2021 14:06:09: 27000000 INFO @ Wed, 08 Dec 2021 14:06:09: 48000000 INFO @ Wed, 08 Dec 2021 14:06:16: 49000000 INFO @ Wed, 08 Dec 2021 14:06:20: 31000000 INFO @ Wed, 08 Dec 2021 14:06:21: 28000000 INFO @ Wed, 08 Dec 2021 14:06:24: 50000000 INFO @ Wed, 08 Dec 2021 14:06:32: 32000000 INFO @ Wed, 08 Dec 2021 14:06:32: 51000000 INFO @ Wed, 08 Dec 2021 14:06:33: 29000000 INFO @ Wed, 08 Dec 2021 14:06:40: 52000000 INFO @ Wed, 08 Dec 2021 14:06:44: 33000000 INFO @ Wed, 08 Dec 2021 14:06:45: 30000000 INFO @ Wed, 08 Dec 2021 14:06:48: 53000000 INFO @ Wed, 08 Dec 2021 14:06:56: 34000000 INFO @ Wed, 08 Dec 2021 14:06:56: 54000000 INFO @ Wed, 08 Dec 2021 14:06:56: 31000000 INFO @ Wed, 08 Dec 2021 14:07:04: 55000000 INFO @ Wed, 08 Dec 2021 14:07:07: 35000000 INFO @ Wed, 08 Dec 2021 14:07:08: 32000000 INFO @ Wed, 08 Dec 2021 14:07:11: 56000000 INFO @ Wed, 08 Dec 2021 14:07:14: #1 tag size is determined as 74 bps INFO @ Wed, 08 Dec 2021 14:07:14: #1 tag size = 74 INFO @ Wed, 08 Dec 2021 14:07:14: #1 total tags in treatment: 27605948 INFO @ Wed, 08 Dec 2021 14:07:14: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:07:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:07:14: #1 tags after filtering in treatment: 24735512 INFO @ Wed, 08 Dec 2021 14:07:14: #1 Redundant rate of treatment: 0.10 INFO @ Wed, 08 Dec 2021 14:07:14: #1 finished! INFO @ Wed, 08 Dec 2021 14:07:14: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:07:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:07:16: #2 number of paired peaks: 176 WARNING @ Wed, 08 Dec 2021 14:07:16: Fewer paired peaks (176) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 176 pairs to build model! INFO @ Wed, 08 Dec 2021 14:07:16: start model_add_line... INFO @ Wed, 08 Dec 2021 14:07:16: start X-correlation... INFO @ Wed, 08 Dec 2021 14:07:16: end of X-cor INFO @ Wed, 08 Dec 2021 14:07:16: #2 finished! INFO @ Wed, 08 Dec 2021 14:07:16: #2 predicted fragment length is 112 bps INFO @ Wed, 08 Dec 2021 14:07:16: #2 alternative fragment length(s) may be 2,74,112 bps INFO @ Wed, 08 Dec 2021 14:07:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.05_model.r WARNING @ Wed, 08 Dec 2021 14:07:17: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 14:07:17: #2 You may need to consider one of the other alternative d(s): 2,74,112 WARNING @ Wed, 08 Dec 2021 14:07:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 14:07:17: #3 Call peaks... INFO @ Wed, 08 Dec 2021 14:07:17: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 14:07:19: 36000000 INFO @ Wed, 08 Dec 2021 14:07:20: 33000000 INFO @ Wed, 08 Dec 2021 14:07:30: 37000000 INFO @ Wed, 08 Dec 2021 14:07:31: 34000000 INFO @ Wed, 08 Dec 2021 14:07:42: 38000000 INFO @ Wed, 08 Dec 2021 14:07:43: 35000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:07:54: 39000000 INFO @ Wed, 08 Dec 2021 14:07:55: 36000000 INFO @ Wed, 08 Dec 2021 14:08:07: 40000000 INFO @ Wed, 08 Dec 2021 14:08:07: 37000000 INFO @ Wed, 08 Dec 2021 14:08:11: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 14:08:18: 41000000 INFO @ Wed, 08 Dec 2021 14:08:19: 38000000 INFO @ Wed, 08 Dec 2021 14:08:29: 42000000 INFO @ Wed, 08 Dec 2021 14:08:30: 39000000 INFO @ Wed, 08 Dec 2021 14:08:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.05_peaks.xls INFO @ Wed, 08 Dec 2021 14:08:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 14:08:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.05_summits.bed INFO @ Wed, 08 Dec 2021 14:08:35: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (735 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:08:41: 43000000 INFO @ Wed, 08 Dec 2021 14:08:42: 40000000 INFO @ Wed, 08 Dec 2021 14:08:52: 44000000 INFO @ Wed, 08 Dec 2021 14:08:53: 41000000 INFO @ Wed, 08 Dec 2021 14:09:04: 45000000 INFO @ Wed, 08 Dec 2021 14:09:05: 42000000 INFO @ Wed, 08 Dec 2021 14:09:16: 46000000 INFO @ Wed, 08 Dec 2021 14:09:17: 43000000 INFO @ Wed, 08 Dec 2021 14:09:27: 47000000 INFO @ Wed, 08 Dec 2021 14:09:29: 44000000 INFO @ Wed, 08 Dec 2021 14:09:38: 48000000 INFO @ Wed, 08 Dec 2021 14:09:40: 45000000 INFO @ Wed, 08 Dec 2021 14:09:50: 49000000 INFO @ Wed, 08 Dec 2021 14:09:52: 46000000 INFO @ Wed, 08 Dec 2021 14:10:02: 50000000 INFO @ Wed, 08 Dec 2021 14:10:04: 47000000 INFO @ Wed, 08 Dec 2021 14:10:13: 51000000 INFO @ Wed, 08 Dec 2021 14:10:16: 48000000 INFO @ Wed, 08 Dec 2021 14:10:25: 52000000 INFO @ Wed, 08 Dec 2021 14:10:27: 49000000 INFO @ Wed, 08 Dec 2021 14:10:36: 53000000 INFO @ Wed, 08 Dec 2021 14:10:37: 50000000 INFO @ Wed, 08 Dec 2021 14:10:47: 54000000 INFO @ Wed, 08 Dec 2021 14:10:49: 51000000 INFO @ Wed, 08 Dec 2021 14:10:59: 55000000 INFO @ Wed, 08 Dec 2021 14:11:01: 52000000 INFO @ Wed, 08 Dec 2021 14:11:11: 56000000 INFO @ Wed, 08 Dec 2021 14:11:12: 53000000 INFO @ Wed, 08 Dec 2021 14:11:13: #1 tag size is determined as 74 bps INFO @ Wed, 08 Dec 2021 14:11:13: #1 tag size = 74 INFO @ Wed, 08 Dec 2021 14:11:13: #1 total tags in treatment: 27605948 INFO @ Wed, 08 Dec 2021 14:11:13: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:11:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:11:14: #1 tags after filtering in treatment: 24735512 INFO @ Wed, 08 Dec 2021 14:11:14: #1 Redundant rate of treatment: 0.10 INFO @ Wed, 08 Dec 2021 14:11:14: #1 finished! INFO @ Wed, 08 Dec 2021 14:11:14: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:11:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:11:16: #2 number of paired peaks: 176 WARNING @ Wed, 08 Dec 2021 14:11:16: Fewer paired peaks (176) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 176 pairs to build model! INFO @ Wed, 08 Dec 2021 14:11:16: start model_add_line... INFO @ Wed, 08 Dec 2021 14:11:16: start X-correlation... INFO @ Wed, 08 Dec 2021 14:11:16: end of X-cor INFO @ Wed, 08 Dec 2021 14:11:16: #2 finished! INFO @ Wed, 08 Dec 2021 14:11:16: #2 predicted fragment length is 112 bps INFO @ Wed, 08 Dec 2021 14:11:16: #2 alternative fragment length(s) may be 2,74,112 bps INFO @ Wed, 08 Dec 2021 14:11:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.10_model.r WARNING @ Wed, 08 Dec 2021 14:11:16: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 14:11:16: #2 You may need to consider one of the other alternative d(s): 2,74,112 WARNING @ Wed, 08 Dec 2021 14:11:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 14:11:16: #3 Call peaks... INFO @ Wed, 08 Dec 2021 14:11:16: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 14:11:23: 54000000 INFO @ Wed, 08 Dec 2021 14:11:33: 55000000 INFO @ Wed, 08 Dec 2021 14:11:43: 56000000 INFO @ Wed, 08 Dec 2021 14:11:46: #1 tag size is determined as 74 bps INFO @ Wed, 08 Dec 2021 14:11:46: #1 tag size = 74 INFO @ Wed, 08 Dec 2021 14:11:46: #1 total tags in treatment: 27605948 INFO @ Wed, 08 Dec 2021 14:11:46: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:11:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:11:46: #1 tags after filtering in treatment: 24735512 INFO @ Wed, 08 Dec 2021 14:11:46: #1 Redundant rate of treatment: 0.10 INFO @ Wed, 08 Dec 2021 14:11:46: #1 finished! INFO @ Wed, 08 Dec 2021 14:11:46: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:11:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:11:48: #2 number of paired peaks: 176 WARNING @ Wed, 08 Dec 2021 14:11:48: Fewer paired peaks (176) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 176 pairs to build model! INFO @ Wed, 08 Dec 2021 14:11:48: start model_add_line... INFO @ Wed, 08 Dec 2021 14:11:48: start X-correlation... INFO @ Wed, 08 Dec 2021 14:11:48: end of X-cor INFO @ Wed, 08 Dec 2021 14:11:48: #2 finished! INFO @ Wed, 08 Dec 2021 14:11:48: #2 predicted fragment length is 112 bps INFO @ Wed, 08 Dec 2021 14:11:48: #2 alternative fragment length(s) may be 2,74,112 bps INFO @ Wed, 08 Dec 2021 14:11:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.20_model.r WARNING @ Wed, 08 Dec 2021 14:11:48: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 14:11:48: #2 You may need to consider one of the other alternative d(s): 2,74,112 WARNING @ Wed, 08 Dec 2021 14:11:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 14:11:48: #3 Call peaks... INFO @ Wed, 08 Dec 2021 14:11:48: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 14:12:11: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 14:12:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.10_peaks.xls INFO @ Wed, 08 Dec 2021 14:12:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 14:12:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.10_summits.bed INFO @ Wed, 08 Dec 2021 14:12:35: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (480 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:12:43: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 14:13:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.20_peaks.xls INFO @ Wed, 08 Dec 2021 14:13:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 14:13:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8948260/SRX8948260.20_summits.bed INFO @ Wed, 08 Dec 2021 14:13:07: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (263 records, 4 fields): 2 millis CompletedMACS2peakCalling