Job ID = 14159520 SRX = SRX8845633 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 34679463 spots for SRR12345940/SRR12345940.sra Written 34679463 spots for SRR12345940/SRR12345940.sra fastq に変換しました。 bowtie でマッピング中... Your job 14159771 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:40 34679463 reads; of these: 34679463 (100.00%) were unpaired; of these: 563408 (1.62%) aligned 0 times 28280722 (81.55%) aligned exactly 1 time 5835333 (16.83%) aligned >1 times 98.38% overall alignment rate Time searching: 00:08:40 Overall time: 00:08:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4355579 / 34116055 = 0.1277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 22:38:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 22:38:16: #1 read tag files... INFO @ Wed, 08 Dec 2021 22:38:16: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 22:38:23: 1000000 INFO @ Wed, 08 Dec 2021 22:38:30: 2000000 INFO @ Wed, 08 Dec 2021 22:38:36: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 22:38:43: 4000000 INFO @ Wed, 08 Dec 2021 22:38:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 22:38:44: #1 read tag files... INFO @ Wed, 08 Dec 2021 22:38:44: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 22:38:50: 5000000 INFO @ Wed, 08 Dec 2021 22:38:51: 1000000 INFO @ Wed, 08 Dec 2021 22:38:58: 6000000 INFO @ Wed, 08 Dec 2021 22:38:58: 2000000 INFO @ Wed, 08 Dec 2021 22:39:05: 3000000 INFO @ Wed, 08 Dec 2021 22:39:05: 7000000 BedGraph に変換中... INFO @ Wed, 08 Dec 2021 22:39:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 22:39:13: 8000000 INFO @ Wed, 08 Dec 2021 22:39:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 22:39:15: #1 read tag files... INFO @ Wed, 08 Dec 2021 22:39:15: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 22:39:18: 5000000 INFO @ Wed, 08 Dec 2021 22:39:21: 9000000 INFO @ Wed, 08 Dec 2021 22:39:22: 1000000 INFO @ Wed, 08 Dec 2021 22:39:26: 6000000 INFO @ Wed, 08 Dec 2021 22:39:29: 10000000 INFO @ Wed, 08 Dec 2021 22:39:30: 2000000 INFO @ Wed, 08 Dec 2021 22:39:33: 7000000 INFO @ Wed, 08 Dec 2021 22:39:37: 3000000 INFO @ Wed, 08 Dec 2021 22:39:37: 11000000 INFO @ Wed, 08 Dec 2021 22:39:40: 8000000 INFO @ Wed, 08 Dec 2021 22:39:44: 4000000 INFO @ Wed, 08 Dec 2021 22:39:45: 12000000 INFO @ Wed, 08 Dec 2021 22:39:47: 9000000 INFO @ Wed, 08 Dec 2021 22:39:50: 5000000 INFO @ Wed, 08 Dec 2021 22:39:52: 13000000 INFO @ Wed, 08 Dec 2021 22:39:55: 10000000 INFO @ Wed, 08 Dec 2021 22:39:58: 6000000 INFO @ Wed, 08 Dec 2021 22:40:01: 14000000 INFO @ Wed, 08 Dec 2021 22:40:02: 11000000 INFO @ Wed, 08 Dec 2021 22:40:05: 7000000 INFO @ Wed, 08 Dec 2021 22:40:09: 15000000 INFO @ Wed, 08 Dec 2021 22:40:09: 12000000 INFO @ Wed, 08 Dec 2021 22:40:12: 8000000 INFO @ Wed, 08 Dec 2021 22:40:16: 13000000 INFO @ Wed, 08 Dec 2021 22:40:17: 16000000 INFO @ Wed, 08 Dec 2021 22:40:19: 9000000 INFO @ Wed, 08 Dec 2021 22:40:24: 14000000 INFO @ Wed, 08 Dec 2021 22:40:25: 17000000 INFO @ Wed, 08 Dec 2021 22:40:27: 10000000 INFO @ Wed, 08 Dec 2021 22:40:31: 15000000 INFO @ Wed, 08 Dec 2021 22:40:33: 18000000 INFO @ Wed, 08 Dec 2021 22:40:34: 11000000 INFO @ Wed, 08 Dec 2021 22:40:38: 16000000 INFO @ Wed, 08 Dec 2021 22:40:41: 19000000 INFO @ Wed, 08 Dec 2021 22:40:41: 12000000 INFO @ Wed, 08 Dec 2021 22:40:46: 17000000 INFO @ Wed, 08 Dec 2021 22:40:48: 13000000 INFO @ Wed, 08 Dec 2021 22:40:48: 20000000 INFO @ Wed, 08 Dec 2021 22:40:53: 18000000 INFO @ Wed, 08 Dec 2021 22:40:56: 14000000 INFO @ Wed, 08 Dec 2021 22:40:57: 21000000 INFO @ Wed, 08 Dec 2021 22:41:00: 19000000 INFO @ Wed, 08 Dec 2021 22:41:03: 15000000 INFO @ Wed, 08 Dec 2021 22:41:05: 22000000 INFO @ Wed, 08 Dec 2021 22:41:07: 20000000 INFO @ Wed, 08 Dec 2021 22:41:10: 16000000 INFO @ Wed, 08 Dec 2021 22:41:13: 23000000 INFO @ Wed, 08 Dec 2021 22:41:14: 21000000 INFO @ Wed, 08 Dec 2021 22:41:17: 17000000 INFO @ Wed, 08 Dec 2021 22:41:20: 24000000 INFO @ Wed, 08 Dec 2021 22:41:21: 22000000 INFO @ Wed, 08 Dec 2021 22:41:24: 18000000 INFO @ Wed, 08 Dec 2021 22:41:28: 23000000 INFO @ Wed, 08 Dec 2021 22:41:29: 25000000 INFO @ Wed, 08 Dec 2021 22:41:31: 19000000 INFO @ Wed, 08 Dec 2021 22:41:35: 24000000 INFO @ Wed, 08 Dec 2021 22:41:37: 26000000 INFO @ Wed, 08 Dec 2021 22:41:38: 20000000 INFO @ Wed, 08 Dec 2021 22:41:42: 25000000 INFO @ Wed, 08 Dec 2021 22:41:45: 27000000 INFO @ Wed, 08 Dec 2021 22:41:45: 21000000 INFO @ Wed, 08 Dec 2021 22:41:49: 26000000 INFO @ Wed, 08 Dec 2021 22:41:52: 22000000 INFO @ Wed, 08 Dec 2021 22:41:53: 28000000 INFO @ Wed, 08 Dec 2021 22:41:57: 27000000 INFO @ Wed, 08 Dec 2021 22:42:00: 23000000 INFO @ Wed, 08 Dec 2021 22:42:01: 29000000 INFO @ Wed, 08 Dec 2021 22:42:04: 28000000 INFO @ Wed, 08 Dec 2021 22:42:07: 24000000 INFO @ Wed, 08 Dec 2021 22:42:08: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 22:42:08: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 22:42:08: #1 total tags in treatment: 29760476 INFO @ Wed, 08 Dec 2021 22:42:08: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 22:42:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 22:42:09: #1 tags after filtering in treatment: 29760476 INFO @ Wed, 08 Dec 2021 22:42:09: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 22:42:09: #1 finished! INFO @ Wed, 08 Dec 2021 22:42:09: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 22:42:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 22:42:11: #2 number of paired peaks: 92 WARNING @ Wed, 08 Dec 2021 22:42:11: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 22:42:11: Process for pairing-model is terminated! INFO @ Wed, 08 Dec 2021 22:42:11: 29000000 cut: /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 22:42:15: 25000000 INFO @ Wed, 08 Dec 2021 22:42:16: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 22:42:16: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 22:42:16: #1 total tags in treatment: 29760476 INFO @ Wed, 08 Dec 2021 22:42:16: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 22:42:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 22:42:17: #1 tags after filtering in treatment: 29760476 INFO @ Wed, 08 Dec 2021 22:42:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 22:42:17: #1 finished! INFO @ Wed, 08 Dec 2021 22:42:17: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 22:42:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 22:42:19: #2 number of paired peaks: 92 WARNING @ Wed, 08 Dec 2021 22:42:19: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 22:42:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 22:42:21: 26000000 INFO @ Wed, 08 Dec 2021 22:42:28: 27000000 INFO @ Wed, 08 Dec 2021 22:42:34: 28000000 INFO @ Wed, 08 Dec 2021 22:42:40: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 22:42:45: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 22:42:45: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 22:42:45: #1 total tags in treatment: 29760476 INFO @ Wed, 08 Dec 2021 22:42:45: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 22:42:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 22:42:46: #1 tags after filtering in treatment: 29760476 INFO @ Wed, 08 Dec 2021 22:42:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 22:42:46: #1 finished! INFO @ Wed, 08 Dec 2021 22:42:46: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 22:42:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 22:42:48: #2 number of paired peaks: 92 WARNING @ Wed, 08 Dec 2021 22:42:48: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 22:42:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8845633/SRX8845633.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。