Job ID = 10165738 SRX = SRX8832091 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31748053 spots for SRR12332049/SRR12332049.sra Written 31748053 spots for SRR12332049/SRR12332049.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166022 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 31748053 reads; of these: 31748053 (100.00%) were unpaired; of these: 4304137 (13.56%) aligned 0 times 23179478 (73.01%) aligned exactly 1 time 4264438 (13.43%) aligned >1 times 86.44% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16980307 / 27443916 = 0.6187 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:56:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:56:27: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:56:27: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:56:32: 1000000 INFO @ Thu, 08 Oct 2020 19:56:38: 2000000 INFO @ Thu, 08 Oct 2020 19:56:43: 3000000 INFO @ Thu, 08 Oct 2020 19:56:48: 4000000 INFO @ Thu, 08 Oct 2020 19:56:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:56:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:56:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:56:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:56:58: 6000000 INFO @ Thu, 08 Oct 2020 19:57:03: 1000000 INFO @ Thu, 08 Oct 2020 19:57:04: 7000000 INFO @ Thu, 08 Oct 2020 19:57:09: 2000000 INFO @ Thu, 08 Oct 2020 19:57:09: 8000000 INFO @ Thu, 08 Oct 2020 19:57:14: 3000000 INFO @ Thu, 08 Oct 2020 19:57:15: 9000000 INFO @ Thu, 08 Oct 2020 19:57:20: 4000000 INFO @ Thu, 08 Oct 2020 19:57:20: 10000000 INFO @ Thu, 08 Oct 2020 19:57:23: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:57:23: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:57:23: #1 total tags in treatment: 10463609 INFO @ Thu, 08 Oct 2020 19:57:23: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:57:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:57:23: #1 tags after filtering in treatment: 10463609 INFO @ Thu, 08 Oct 2020 19:57:23: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:57:23: #1 finished! INFO @ Thu, 08 Oct 2020 19:57:23: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:57:23: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:57:24: #2 number of paired peaks: 5801 INFO @ Thu, 08 Oct 2020 19:57:24: start model_add_line... INFO @ Thu, 08 Oct 2020 19:57:24: start X-correlation... INFO @ Thu, 08 Oct 2020 19:57:24: end of X-cor INFO @ Thu, 08 Oct 2020 19:57:24: #2 finished! INFO @ Thu, 08 Oct 2020 19:57:24: #2 predicted fragment length is 148 bps INFO @ Thu, 08 Oct 2020 19:57:24: #2 alternative fragment length(s) may be 148 bps INFO @ Thu, 08 Oct 2020 19:57:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.05_model.r INFO @ Thu, 08 Oct 2020 19:57:24: #3 Call peaks... INFO @ Thu, 08 Oct 2020 19:57:24: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 19:57:25: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:57:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:57:27: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:57:27: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:57:31: 6000000 INFO @ Thu, 08 Oct 2020 19:57:33: 1000000 INFO @ Thu, 08 Oct 2020 19:57:36: 7000000 INFO @ Thu, 08 Oct 2020 19:57:39: 2000000 INFO @ Thu, 08 Oct 2020 19:57:42: 8000000 INFO @ Thu, 08 Oct 2020 19:57:44: 3000000 INFO @ Thu, 08 Oct 2020 19:57:47: 9000000 INFO @ Thu, 08 Oct 2020 19:57:49: 4000000 INFO @ Thu, 08 Oct 2020 19:57:52: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 19:57:53: 10000000 INFO @ Thu, 08 Oct 2020 19:57:55: 5000000 INFO @ Thu, 08 Oct 2020 19:57:56: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:57:56: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:57:56: #1 total tags in treatment: 10463609 INFO @ Thu, 08 Oct 2020 19:57:56: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:57:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:57:56: #1 tags after filtering in treatment: 10463609 INFO @ Thu, 08 Oct 2020 19:57:56: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:57:56: #1 finished! INFO @ Thu, 08 Oct 2020 19:57:56: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:57:56: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:57:57: #2 number of paired peaks: 5801 INFO @ Thu, 08 Oct 2020 19:57:57: start model_add_line... INFO @ Thu, 08 Oct 2020 19:57:57: start X-correlation... INFO @ Thu, 08 Oct 2020 19:57:57: end of X-cor INFO @ Thu, 08 Oct 2020 19:57:57: #2 finished! INFO @ Thu, 08 Oct 2020 19:57:57: #2 predicted fragment length is 148 bps INFO @ Thu, 08 Oct 2020 19:57:57: #2 alternative fragment length(s) may be 148 bps INFO @ Thu, 08 Oct 2020 19:57:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.10_model.r INFO @ Thu, 08 Oct 2020 19:57:57: #3 Call peaks... INFO @ Thu, 08 Oct 2020 19:57:57: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 19:58:00: 6000000 INFO @ Thu, 08 Oct 2020 19:58:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.05_peaks.xls INFO @ Thu, 08 Oct 2020 19:58:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.05_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 19:58:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.05_summits.bed INFO @ Thu, 08 Oct 2020 19:58:04: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10217 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:58:06: 7000000 INFO @ Thu, 08 Oct 2020 19:58:11: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:58:16: 9000000 INFO @ Thu, 08 Oct 2020 19:58:21: 10000000 INFO @ Thu, 08 Oct 2020 19:58:24: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:58:24: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:58:24: #1 total tags in treatment: 10463609 INFO @ Thu, 08 Oct 2020 19:58:24: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:58:24: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 19:58:24: #1 tags after filtering in treatment: 10463609 INFO @ Thu, 08 Oct 2020 19:58:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:58:24: #1 finished! INFO @ Thu, 08 Oct 2020 19:58:24: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:58:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:58:25: #2 number of paired peaks: 5801 INFO @ Thu, 08 Oct 2020 19:58:25: start model_add_line... INFO @ Thu, 08 Oct 2020 19:58:25: start X-correlation... INFO @ Thu, 08 Oct 2020 19:58:25: end of X-cor INFO @ Thu, 08 Oct 2020 19:58:25: #2 finished! INFO @ Thu, 08 Oct 2020 19:58:25: #2 predicted fragment length is 148 bps INFO @ Thu, 08 Oct 2020 19:58:25: #2 alternative fragment length(s) may be 148 bps INFO @ Thu, 08 Oct 2020 19:58:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.20_model.r INFO @ Thu, 08 Oct 2020 19:58:25: #3 Call peaks... INFO @ Thu, 08 Oct 2020 19:58:25: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:58:36: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.10_peaks.xls INFO @ Thu, 08 Oct 2020 19:58:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.10_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 19:58:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.10_summits.bed INFO @ Thu, 08 Oct 2020 19:58:36: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (8674 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:58:52: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 19:59:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.20_peaks.xls INFO @ Thu, 08 Oct 2020 19:59:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.20_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 19:59:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832091/SRX8832091.20_summits.bed INFO @ Thu, 08 Oct 2020 19:59:04: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (7032 records, 4 fields): 9 millis CompletedMACS2peakCalling