Job ID = 14160263 SRX = SRX8832086 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11817326 spots for SRR12332044/SRR12332044.sra Written 11817326 spots for SRR12332044/SRR12332044.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160381 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:07 11817326 reads; of these: 11817326 (100.00%) were unpaired; of these: 2544580 (21.53%) aligned 0 times 7891384 (66.78%) aligned exactly 1 time 1381362 (11.69%) aligned >1 times 78.47% overall alignment rate Time searching: 00:02:07 Overall time: 00:02:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 5475403 / 9272746 = 0.5905 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:08:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:08:20: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:08:20: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:08:26: 1000000 INFO @ Thu, 09 Dec 2021 01:08:31: 2000000 INFO @ Thu, 09 Dec 2021 01:08:36: 3000000 INFO @ Thu, 09 Dec 2021 01:08:40: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:08:40: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:08:40: #1 total tags in treatment: 3797343 INFO @ Thu, 09 Dec 2021 01:08:40: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:08:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:08:41: #1 tags after filtering in treatment: 3797343 INFO @ Thu, 09 Dec 2021 01:08:41: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:08:41: #1 finished! INFO @ Thu, 09 Dec 2021 01:08:41: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:08:41: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:08:41: #2 number of paired peaks: 4203 INFO @ Thu, 09 Dec 2021 01:08:41: start model_add_line... INFO @ Thu, 09 Dec 2021 01:08:41: start X-correlation... INFO @ Thu, 09 Dec 2021 01:08:41: end of X-cor INFO @ Thu, 09 Dec 2021 01:08:41: #2 finished! INFO @ Thu, 09 Dec 2021 01:08:41: #2 predicted fragment length is 168 bps INFO @ Thu, 09 Dec 2021 01:08:41: #2 alternative fragment length(s) may be 168 bps INFO @ Thu, 09 Dec 2021 01:08:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_model.r INFO @ Thu, 09 Dec 2021 01:08:41: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:08:41: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:08:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:08:50: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:08:50: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:08:52: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:08:56: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:08:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:08:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_summits.bed INFO @ Thu, 09 Dec 2021 01:08:56: 1000000 INFO @ Thu, 09 Dec 2021 01:08:56: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (5960 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:09:03: 2000000 INFO @ Thu, 09 Dec 2021 01:09:10: 3000000 INFO @ Thu, 09 Dec 2021 01:09:15: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:09:15: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:09:15: #1 total tags in treatment: 3797343 INFO @ Thu, 09 Dec 2021 01:09:15: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:09:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:09:15: #1 tags after filtering in treatment: 3797343 INFO @ Thu, 09 Dec 2021 01:09:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:09:15: #1 finished! INFO @ Thu, 09 Dec 2021 01:09:15: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:09:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:09:16: #2 number of paired peaks: 4203 INFO @ Thu, 09 Dec 2021 01:09:16: start model_add_line... INFO @ Thu, 09 Dec 2021 01:09:16: start X-correlation... INFO @ Thu, 09 Dec 2021 01:09:16: end of X-cor INFO @ Thu, 09 Dec 2021 01:09:16: #2 finished! INFO @ Thu, 09 Dec 2021 01:09:16: #2 predicted fragment length is 168 bps INFO @ Thu, 09 Dec 2021 01:09:16: #2 alternative fragment length(s) may be 168 bps INFO @ Thu, 09 Dec 2021 01:09:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_model.r INFO @ Thu, 09 Dec 2021 01:09:16: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:09:16: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:09:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:09:20: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:09:20: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:09:27: 1000000 INFO @ Thu, 09 Dec 2021 01:09:27: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:09:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:09:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:09:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_summits.bed INFO @ Thu, 09 Dec 2021 01:09:31: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (4856 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:09:33: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:09:40: 3000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:09:45: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:09:45: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:09:45: #1 total tags in treatment: 3797343 INFO @ Thu, 09 Dec 2021 01:09:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:09:45: #1 tags after filtering in treatment: 3797343 INFO @ Thu, 09 Dec 2021 01:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:09:45: #1 finished! INFO @ Thu, 09 Dec 2021 01:09:45: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:09:46: #2 number of paired peaks: 4203 INFO @ Thu, 09 Dec 2021 01:09:46: start model_add_line... INFO @ Thu, 09 Dec 2021 01:09:46: start X-correlation... INFO @ Thu, 09 Dec 2021 01:09:46: end of X-cor INFO @ Thu, 09 Dec 2021 01:09:46: #2 finished! INFO @ Thu, 09 Dec 2021 01:09:46: #2 predicted fragment length is 168 bps INFO @ Thu, 09 Dec 2021 01:09:46: #2 alternative fragment length(s) may be 168 bps INFO @ Thu, 09 Dec 2021 01:09:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_model.r INFO @ Thu, 09 Dec 2021 01:09:46: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:09:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:09:56: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:10:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:10:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:10:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_summits.bed INFO @ Thu, 09 Dec 2021 01:10:00: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3702 records, 4 fields): 5 millis CompletedMACS2peakCalling