Job ID = 10165732 SRX = SRX8832086 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11817326 spots for SRR12332044/SRR12332044.sra Written 11817326 spots for SRR12332044/SRR12332044.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165954 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:32 11817326 reads; of these: 11817326 (100.00%) were unpaired; of these: 2544580 (21.53%) aligned 0 times 7891384 (66.78%) aligned exactly 1 time 1381362 (11.69%) aligned >1 times 78.47% overall alignment rate Time searching: 00:02:32 Overall time: 00:02:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 5475403 / 9272746 = 0.5905 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:47:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:47:01: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:47:01: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:47:07: 1000000 INFO @ Thu, 08 Oct 2020 19:47:12: 2000000 INFO @ Thu, 08 Oct 2020 19:47:18: 3000000 INFO @ Thu, 08 Oct 2020 19:47:22: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:47:22: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:47:22: #1 total tags in treatment: 3797343 INFO @ Thu, 08 Oct 2020 19:47:22: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:47:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:47:22: #1 tags after filtering in treatment: 3797343 INFO @ Thu, 08 Oct 2020 19:47:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:47:22: #1 finished! INFO @ Thu, 08 Oct 2020 19:47:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:47:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:47:22: #2 number of paired peaks: 4203 INFO @ Thu, 08 Oct 2020 19:47:22: start model_add_line... INFO @ Thu, 08 Oct 2020 19:47:22: start X-correlation... INFO @ Thu, 08 Oct 2020 19:47:22: end of X-cor INFO @ Thu, 08 Oct 2020 19:47:22: #2 finished! INFO @ Thu, 08 Oct 2020 19:47:22: #2 predicted fragment length is 168 bps INFO @ Thu, 08 Oct 2020 19:47:22: #2 alternative fragment length(s) may be 168 bps INFO @ Thu, 08 Oct 2020 19:47:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_model.r INFO @ Thu, 08 Oct 2020 19:47:23: #3 Call peaks... INFO @ Thu, 08 Oct 2020 19:47:23: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:47:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:47:31: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:47:31: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:47:33: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 19:47:37: 1000000 INFO @ Thu, 08 Oct 2020 19:47:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_peaks.xls INFO @ Thu, 08 Oct 2020 19:47:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 19:47:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.05_summits.bed INFO @ Thu, 08 Oct 2020 19:47:38: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5960 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:47:44: 2000000 INFO @ Thu, 08 Oct 2020 19:47:50: 3000000 INFO @ Thu, 08 Oct 2020 19:47:55: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:47:55: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:47:55: #1 total tags in treatment: 3797343 INFO @ Thu, 08 Oct 2020 19:47:55: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:47:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:47:55: #1 tags after filtering in treatment: 3797343 INFO @ Thu, 08 Oct 2020 19:47:55: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:47:55: #1 finished! INFO @ Thu, 08 Oct 2020 19:47:55: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:47:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:47:56: #2 number of paired peaks: 4203 INFO @ Thu, 08 Oct 2020 19:47:56: start model_add_line... INFO @ Thu, 08 Oct 2020 19:47:56: start X-correlation... INFO @ Thu, 08 Oct 2020 19:47:56: end of X-cor INFO @ Thu, 08 Oct 2020 19:47:56: #2 finished! INFO @ Thu, 08 Oct 2020 19:47:56: #2 predicted fragment length is 168 bps INFO @ Thu, 08 Oct 2020 19:47:56: #2 alternative fragment length(s) may be 168 bps INFO @ Thu, 08 Oct 2020 19:47:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_model.r INFO @ Thu, 08 Oct 2020 19:47:56: #3 Call peaks... INFO @ Thu, 08 Oct 2020 19:47:56: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:48:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:48:01: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:48:01: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:48:06: 1000000 INFO @ Thu, 08 Oct 2020 19:48:07: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 19:48:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_peaks.xls INFO @ Thu, 08 Oct 2020 19:48:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 19:48:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.10_summits.bed INFO @ Thu, 08 Oct 2020 19:48:11: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (4856 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:48:12: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:48:17: 3000000 INFO @ Thu, 08 Oct 2020 19:48:21: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:48:21: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:48:21: #1 total tags in treatment: 3797343 INFO @ Thu, 08 Oct 2020 19:48:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:48:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:48:21: #1 tags after filtering in treatment: 3797343 INFO @ Thu, 08 Oct 2020 19:48:21: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:48:21: #1 finished! INFO @ Thu, 08 Oct 2020 19:48:21: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:48:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:48:22: #2 number of paired peaks: 4203 INFO @ Thu, 08 Oct 2020 19:48:22: start model_add_line... INFO @ Thu, 08 Oct 2020 19:48:22: start X-correlation... INFO @ Thu, 08 Oct 2020 19:48:22: end of X-cor INFO @ Thu, 08 Oct 2020 19:48:22: #2 finished! INFO @ Thu, 08 Oct 2020 19:48:22: #2 predicted fragment length is 168 bps INFO @ Thu, 08 Oct 2020 19:48:22: #2 alternative fragment length(s) may be 168 bps INFO @ Thu, 08 Oct 2020 19:48:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_model.r INFO @ Thu, 08 Oct 2020 19:48:22: #3 Call peaks... INFO @ Thu, 08 Oct 2020 19:48:22: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:48:34: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 19:48:39: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_peaks.xls INFO @ Thu, 08 Oct 2020 19:48:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 19:48:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832086/SRX8832086.20_summits.bed INFO @ Thu, 08 Oct 2020 19:48:39: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3702 records, 4 fields): 6 millis CompletedMACS2peakCalling