Job ID = 14160148 SRX = SRX8832078 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20915804 spots for SRR12332020/SRR12332020.sra Written 20915804 spots for SRR12332020/SRR12332020.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160374 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:35 20915804 reads; of these: 20915804 (100.00%) were unpaired; of these: 9771785 (46.72%) aligned 0 times 6211197 (29.70%) aligned exactly 1 time 4932822 (23.58%) aligned >1 times 53.28% overall alignment rate Time searching: 00:03:35 Overall time: 00:03:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3841249 / 11144019 = 0.3447 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:06:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:06:19: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:06:19: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:06:26: 1000000 INFO @ Thu, 09 Dec 2021 01:06:33: 2000000 INFO @ Thu, 09 Dec 2021 01:06:40: 3000000 INFO @ Thu, 09 Dec 2021 01:06:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:06:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:06:49: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:06:49: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:06:54: 5000000 INFO @ Thu, 09 Dec 2021 01:06:56: 1000000 INFO @ Thu, 09 Dec 2021 01:07:02: 6000000 INFO @ Thu, 09 Dec 2021 01:07:03: 2000000 INFO @ Thu, 09 Dec 2021 01:07:09: 7000000 INFO @ Thu, 09 Dec 2021 01:07:09: 3000000 INFO @ Thu, 09 Dec 2021 01:07:11: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:07:11: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:07:11: #1 total tags in treatment: 7302770 INFO @ Thu, 09 Dec 2021 01:07:11: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:07:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:07:11: #1 tags after filtering in treatment: 7302770 INFO @ Thu, 09 Dec 2021 01:07:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:07:11: #1 finished! INFO @ Thu, 09 Dec 2021 01:07:11: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:07:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:07:12: #2 number of paired peaks: 4642 INFO @ Thu, 09 Dec 2021 01:07:12: start model_add_line... INFO @ Thu, 09 Dec 2021 01:07:12: start X-correlation... INFO @ Thu, 09 Dec 2021 01:07:12: end of X-cor INFO @ Thu, 09 Dec 2021 01:07:12: #2 finished! INFO @ Thu, 09 Dec 2021 01:07:12: #2 predicted fragment length is 141 bps INFO @ Thu, 09 Dec 2021 01:07:12: #2 alternative fragment length(s) may be 141 bps INFO @ Thu, 09 Dec 2021 01:07:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.05_model.r INFO @ Thu, 09 Dec 2021 01:07:12: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:07:12: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:07:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:07:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:07:19: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:07:19: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:07:22: 5000000 INFO @ Thu, 09 Dec 2021 01:07:25: 1000000 INFO @ Thu, 09 Dec 2021 01:07:29: 6000000 INFO @ Thu, 09 Dec 2021 01:07:32: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:07:32: 2000000 INFO @ Thu, 09 Dec 2021 01:07:35: 7000000 INFO @ Thu, 09 Dec 2021 01:07:37: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:07:37: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:07:37: #1 total tags in treatment: 7302770 INFO @ Thu, 09 Dec 2021 01:07:37: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:07:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:07:37: #1 tags after filtering in treatment: 7302770 INFO @ Thu, 09 Dec 2021 01:07:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:07:37: #1 finished! INFO @ Thu, 09 Dec 2021 01:07:37: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:07:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:07:38: #2 number of paired peaks: 4642 INFO @ Thu, 09 Dec 2021 01:07:38: start model_add_line... INFO @ Thu, 09 Dec 2021 01:07:38: start X-correlation... INFO @ Thu, 09 Dec 2021 01:07:38: end of X-cor INFO @ Thu, 09 Dec 2021 01:07:38: #2 finished! INFO @ Thu, 09 Dec 2021 01:07:38: #2 predicted fragment length is 141 bps INFO @ Thu, 09 Dec 2021 01:07:38: #2 alternative fragment length(s) may be 141 bps INFO @ Thu, 09 Dec 2021 01:07:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.10_model.r INFO @ Thu, 09 Dec 2021 01:07:38: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:07:38: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:07:38: 3000000 INFO @ Thu, 09 Dec 2021 01:07:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:07:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:07:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.05_summits.bed INFO @ Thu, 09 Dec 2021 01:07:40: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (7506 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:07:44: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:07:50: 5000000 INFO @ Thu, 09 Dec 2021 01:07:55: 6000000 INFO @ Thu, 09 Dec 2021 01:07:57: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:08:01: 7000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:08:03: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:08:03: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:08:03: #1 total tags in treatment: 7302770 INFO @ Thu, 09 Dec 2021 01:08:03: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:08:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:08:03: #1 tags after filtering in treatment: 7302770 INFO @ Thu, 09 Dec 2021 01:08:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:08:03: #1 finished! INFO @ Thu, 09 Dec 2021 01:08:03: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:08:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:08:04: #2 number of paired peaks: 4642 INFO @ Thu, 09 Dec 2021 01:08:04: start model_add_line... INFO @ Thu, 09 Dec 2021 01:08:04: start X-correlation... INFO @ Thu, 09 Dec 2021 01:08:04: end of X-cor INFO @ Thu, 09 Dec 2021 01:08:04: #2 finished! INFO @ Thu, 09 Dec 2021 01:08:04: #2 predicted fragment length is 141 bps INFO @ Thu, 09 Dec 2021 01:08:04: #2 alternative fragment length(s) may be 141 bps INFO @ Thu, 09 Dec 2021 01:08:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.20_model.r INFO @ Thu, 09 Dec 2021 01:08:04: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:08:04: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:08:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:08:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:08:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.10_summits.bed INFO @ Thu, 09 Dec 2021 01:08:05: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (5030 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:08:23: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:08:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:08:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:08:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8832078/SRX8832078.20_summits.bed INFO @ Thu, 09 Dec 2021 01:08:31: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2576 records, 4 fields): 13 millis CompletedMACS2peakCalling