Job ID = 14160399 SRX = SRX8582555 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 49952958 spots for SRR12054116/SRR12054116.sra Written 49952958 spots for SRR12054116/SRR12054116.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160548 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:40 49952958 reads; of these: 49952958 (100.00%) were unpaired; of these: 412622 (0.83%) aligned 0 times 40742954 (81.56%) aligned exactly 1 time 8797382 (17.61%) aligned >1 times 99.17% overall alignment rate Time searching: 00:10:40 Overall time: 00:10:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10677667 / 49540336 = 0.2155 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:33:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:33:24: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:33:24: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:33:29: 1000000 INFO @ Thu, 09 Dec 2021 02:33:35: 2000000 INFO @ Thu, 09 Dec 2021 02:33:40: 3000000 INFO @ Thu, 09 Dec 2021 02:33:46: 4000000 INFO @ Thu, 09 Dec 2021 02:33:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:33:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:33:54: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:33:54: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:33:56: 6000000 INFO @ Thu, 09 Dec 2021 02:34:00: 1000000 INFO @ Thu, 09 Dec 2021 02:34:02: 7000000 INFO @ Thu, 09 Dec 2021 02:34:06: 2000000 INFO @ Thu, 09 Dec 2021 02:34:08: 8000000 INFO @ Thu, 09 Dec 2021 02:34:12: 3000000 INFO @ Thu, 09 Dec 2021 02:34:14: 9000000 INFO @ Thu, 09 Dec 2021 02:34:18: 4000000 INFO @ Thu, 09 Dec 2021 02:34:21: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:34:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:34:24: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:34:24: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:34:24: 5000000 INFO @ Thu, 09 Dec 2021 02:34:27: 11000000 INFO @ Thu, 09 Dec 2021 02:34:31: 6000000 INFO @ Thu, 09 Dec 2021 02:34:31: 1000000 INFO @ Thu, 09 Dec 2021 02:34:34: 12000000 INFO @ Thu, 09 Dec 2021 02:34:38: 7000000 INFO @ Thu, 09 Dec 2021 02:34:39: 2000000 INFO @ Thu, 09 Dec 2021 02:34:40: 13000000 INFO @ Thu, 09 Dec 2021 02:34:44: 8000000 INFO @ Thu, 09 Dec 2021 02:34:46: 3000000 INFO @ Thu, 09 Dec 2021 02:34:47: 14000000 INFO @ Thu, 09 Dec 2021 02:34:51: 9000000 INFO @ Thu, 09 Dec 2021 02:34:53: 4000000 INFO @ Thu, 09 Dec 2021 02:34:54: 15000000 INFO @ Thu, 09 Dec 2021 02:34:58: 10000000 INFO @ Thu, 09 Dec 2021 02:35:01: 16000000 INFO @ Thu, 09 Dec 2021 02:35:01: 5000000 INFO @ Thu, 09 Dec 2021 02:35:05: 11000000 INFO @ Thu, 09 Dec 2021 02:35:07: 17000000 INFO @ Thu, 09 Dec 2021 02:35:08: 6000000 INFO @ Thu, 09 Dec 2021 02:35:12: 12000000 INFO @ Thu, 09 Dec 2021 02:35:14: 18000000 INFO @ Thu, 09 Dec 2021 02:35:15: 7000000 INFO @ Thu, 09 Dec 2021 02:35:18: 13000000 INFO @ Thu, 09 Dec 2021 02:35:21: 19000000 INFO @ Thu, 09 Dec 2021 02:35:23: 8000000 INFO @ Thu, 09 Dec 2021 02:35:25: 14000000 INFO @ Thu, 09 Dec 2021 02:35:28: 20000000 INFO @ Thu, 09 Dec 2021 02:35:30: 9000000 INFO @ Thu, 09 Dec 2021 02:35:32: 15000000 INFO @ Thu, 09 Dec 2021 02:35:35: 21000000 INFO @ Thu, 09 Dec 2021 02:35:37: 10000000 INFO @ Thu, 09 Dec 2021 02:35:39: 16000000 INFO @ Thu, 09 Dec 2021 02:35:42: 22000000 INFO @ Thu, 09 Dec 2021 02:35:45: 11000000 INFO @ Thu, 09 Dec 2021 02:35:46: 17000000 INFO @ Thu, 09 Dec 2021 02:35:48: 23000000 INFO @ Thu, 09 Dec 2021 02:35:52: 12000000 INFO @ Thu, 09 Dec 2021 02:35:54: 18000000 INFO @ Thu, 09 Dec 2021 02:35:55: 24000000 INFO @ Thu, 09 Dec 2021 02:35:59: 13000000 INFO @ Thu, 09 Dec 2021 02:36:01: 25000000 INFO @ Thu, 09 Dec 2021 02:36:02: 19000000 INFO @ Thu, 09 Dec 2021 02:36:06: 14000000 INFO @ Thu, 09 Dec 2021 02:36:08: 26000000 INFO @ Thu, 09 Dec 2021 02:36:09: 20000000 INFO @ Thu, 09 Dec 2021 02:36:13: 15000000 INFO @ Thu, 09 Dec 2021 02:36:14: 27000000 INFO @ Thu, 09 Dec 2021 02:36:17: 21000000 INFO @ Thu, 09 Dec 2021 02:36:21: 28000000 INFO @ Thu, 09 Dec 2021 02:36:21: 16000000 INFO @ Thu, 09 Dec 2021 02:36:25: 22000000 INFO @ Thu, 09 Dec 2021 02:36:27: 29000000 INFO @ Thu, 09 Dec 2021 02:36:28: 17000000 INFO @ Thu, 09 Dec 2021 02:36:33: 23000000 INFO @ Thu, 09 Dec 2021 02:36:34: 30000000 INFO @ Thu, 09 Dec 2021 02:36:35: 18000000 INFO @ Thu, 09 Dec 2021 02:36:40: 24000000 INFO @ Thu, 09 Dec 2021 02:36:41: 31000000 INFO @ Thu, 09 Dec 2021 02:36:42: 19000000 INFO @ Thu, 09 Dec 2021 02:36:47: 32000000 INFO @ Thu, 09 Dec 2021 02:36:48: 25000000 INFO @ Thu, 09 Dec 2021 02:36:49: 20000000 INFO @ Thu, 09 Dec 2021 02:36:54: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:36:56: 26000000 INFO @ Thu, 09 Dec 2021 02:36:56: 21000000 INFO @ Thu, 09 Dec 2021 02:37:01: 34000000 INFO @ Thu, 09 Dec 2021 02:37:03: 22000000 INFO @ Thu, 09 Dec 2021 02:37:04: 27000000 INFO @ Thu, 09 Dec 2021 02:37:07: 35000000 INFO @ Thu, 09 Dec 2021 02:37:10: 23000000 INFO @ Thu, 09 Dec 2021 02:37:11: 28000000 INFO @ Thu, 09 Dec 2021 02:37:14: 36000000 INFO @ Thu, 09 Dec 2021 02:37:17: 24000000 INFO @ Thu, 09 Dec 2021 02:37:20: 29000000 INFO @ Thu, 09 Dec 2021 02:37:21: 37000000 INFO @ Thu, 09 Dec 2021 02:37:25: 25000000 INFO @ Thu, 09 Dec 2021 02:37:27: 38000000 INFO @ Thu, 09 Dec 2021 02:37:28: 30000000 INFO @ Thu, 09 Dec 2021 02:37:32: 26000000 INFO @ Thu, 09 Dec 2021 02:37:33: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:37:33: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:37:33: #1 total tags in treatment: 38862669 INFO @ Thu, 09 Dec 2021 02:37:33: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:37:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:37:34: #1 tags after filtering in treatment: 38862669 INFO @ Thu, 09 Dec 2021 02:37:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:37:34: #1 finished! INFO @ Thu, 09 Dec 2021 02:37:34: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:37:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:37:36: 31000000 INFO @ Thu, 09 Dec 2021 02:37:36: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:37:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:37:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:37:39: 27000000 INFO @ Thu, 09 Dec 2021 02:37:44: 32000000 INFO @ Thu, 09 Dec 2021 02:37:46: 28000000 INFO @ Thu, 09 Dec 2021 02:37:52: 33000000 INFO @ Thu, 09 Dec 2021 02:37:53: 29000000 INFO @ Thu, 09 Dec 2021 02:38:00: 30000000 INFO @ Thu, 09 Dec 2021 02:38:00: 34000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:38:07: 31000000 INFO @ Thu, 09 Dec 2021 02:38:08: 35000000 INFO @ Thu, 09 Dec 2021 02:38:15: 32000000 INFO @ Thu, 09 Dec 2021 02:38:16: 36000000 INFO @ Thu, 09 Dec 2021 02:38:22: 33000000 INFO @ Thu, 09 Dec 2021 02:38:24: 37000000 INFO @ Thu, 09 Dec 2021 02:38:29: 34000000 INFO @ Thu, 09 Dec 2021 02:38:33: 38000000 INFO @ Thu, 09 Dec 2021 02:38:37: 35000000 INFO @ Thu, 09 Dec 2021 02:38:40: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:38:40: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:38:40: #1 total tags in treatment: 38862669 INFO @ Thu, 09 Dec 2021 02:38:40: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:38:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:38:40: #1 tags after filtering in treatment: 38862669 INFO @ Thu, 09 Dec 2021 02:38:40: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:38:40: #1 finished! INFO @ Thu, 09 Dec 2021 02:38:40: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:38:40: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:38:43: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:38:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:38:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:38:43: 36000000 INFO @ Thu, 09 Dec 2021 02:38:50: 37000000 INFO @ Thu, 09 Dec 2021 02:38:57: 38000000 INFO @ Thu, 09 Dec 2021 02:39:02: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:39:02: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:39:02: #1 total tags in treatment: 38862669 INFO @ Thu, 09 Dec 2021 02:39:02: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:39:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:39:03: #1 tags after filtering in treatment: 38862669 INFO @ Thu, 09 Dec 2021 02:39:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:39:03: #1 finished! INFO @ Thu, 09 Dec 2021 02:39:03: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:39:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:39:05: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:39:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:39:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582555/SRX8582555.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling