Job ID = 14160392 SRX = SRX8582551 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 49522675 spots for SRR12054112/SRR12054112.sra Written 49522675 spots for SRR12054112/SRR12054112.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160538 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:46 49522675 reads; of these: 49522675 (100.00%) were unpaired; of these: 478990 (0.97%) aligned 0 times 40201049 (81.18%) aligned exactly 1 time 8842636 (17.86%) aligned >1 times 99.03% overall alignment rate Time searching: 00:10:46 Overall time: 00:10:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12577959 / 49043685 = 0.2565 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:25:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:25:37: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:25:37: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:25:43: 1000000 INFO @ Thu, 09 Dec 2021 02:25:48: 2000000 INFO @ Thu, 09 Dec 2021 02:25:53: 3000000 INFO @ Thu, 09 Dec 2021 02:25:58: 4000000 INFO @ Thu, 09 Dec 2021 02:26:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:26:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:26:08: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:26:08: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:26:08: 6000000 INFO @ Thu, 09 Dec 2021 02:26:13: 1000000 INFO @ Thu, 09 Dec 2021 02:26:13: 7000000 INFO @ Thu, 09 Dec 2021 02:26:18: 2000000 INFO @ Thu, 09 Dec 2021 02:26:18: 8000000 INFO @ Thu, 09 Dec 2021 02:26:22: 3000000 INFO @ Thu, 09 Dec 2021 02:26:23: 9000000 INFO @ Thu, 09 Dec 2021 02:26:27: 4000000 INFO @ Thu, 09 Dec 2021 02:26:28: 10000000 INFO @ Thu, 09 Dec 2021 02:26:31: 5000000 INFO @ Thu, 09 Dec 2021 02:26:34: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:26:36: 6000000 INFO @ Thu, 09 Dec 2021 02:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:26:38: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:26:38: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:26:39: 12000000 INFO @ Thu, 09 Dec 2021 02:26:40: 7000000 INFO @ Thu, 09 Dec 2021 02:26:43: 1000000 INFO @ Thu, 09 Dec 2021 02:26:44: 13000000 INFO @ Thu, 09 Dec 2021 02:26:45: 8000000 INFO @ Thu, 09 Dec 2021 02:26:48: 2000000 INFO @ Thu, 09 Dec 2021 02:26:49: 14000000 INFO @ Thu, 09 Dec 2021 02:26:49: 9000000 INFO @ Thu, 09 Dec 2021 02:26:54: 3000000 INFO @ Thu, 09 Dec 2021 02:26:54: 10000000 INFO @ Thu, 09 Dec 2021 02:26:54: 15000000 INFO @ Thu, 09 Dec 2021 02:26:58: 11000000 INFO @ Thu, 09 Dec 2021 02:26:59: 4000000 INFO @ Thu, 09 Dec 2021 02:26:59: 16000000 INFO @ Thu, 09 Dec 2021 02:27:03: 12000000 INFO @ Thu, 09 Dec 2021 02:27:04: 5000000 INFO @ Thu, 09 Dec 2021 02:27:04: 17000000 INFO @ Thu, 09 Dec 2021 02:27:07: 13000000 INFO @ Thu, 09 Dec 2021 02:27:09: 6000000 INFO @ Thu, 09 Dec 2021 02:27:10: 18000000 INFO @ Thu, 09 Dec 2021 02:27:12: 14000000 INFO @ Thu, 09 Dec 2021 02:27:14: 7000000 INFO @ Thu, 09 Dec 2021 02:27:15: 19000000 INFO @ Thu, 09 Dec 2021 02:27:17: 15000000 INFO @ Thu, 09 Dec 2021 02:27:20: 8000000 INFO @ Thu, 09 Dec 2021 02:27:20: 20000000 INFO @ Thu, 09 Dec 2021 02:27:21: 16000000 INFO @ Thu, 09 Dec 2021 02:27:25: 9000000 INFO @ Thu, 09 Dec 2021 02:27:25: 21000000 INFO @ Thu, 09 Dec 2021 02:27:26: 17000000 INFO @ Thu, 09 Dec 2021 02:27:30: 10000000 INFO @ Thu, 09 Dec 2021 02:27:30: 22000000 INFO @ Thu, 09 Dec 2021 02:27:30: 18000000 INFO @ Thu, 09 Dec 2021 02:27:35: 19000000 INFO @ Thu, 09 Dec 2021 02:27:35: 11000000 INFO @ Thu, 09 Dec 2021 02:27:35: 23000000 INFO @ Thu, 09 Dec 2021 02:27:39: 20000000 INFO @ Thu, 09 Dec 2021 02:27:41: 24000000 INFO @ Thu, 09 Dec 2021 02:27:41: 12000000 INFO @ Thu, 09 Dec 2021 02:27:44: 21000000 INFO @ Thu, 09 Dec 2021 02:27:46: 25000000 INFO @ Thu, 09 Dec 2021 02:27:46: 13000000 INFO @ Thu, 09 Dec 2021 02:27:48: 22000000 INFO @ Thu, 09 Dec 2021 02:27:51: 26000000 INFO @ Thu, 09 Dec 2021 02:27:51: 14000000 INFO @ Thu, 09 Dec 2021 02:27:53: 23000000 INFO @ Thu, 09 Dec 2021 02:27:56: 27000000 INFO @ Thu, 09 Dec 2021 02:27:56: 15000000 INFO @ Thu, 09 Dec 2021 02:27:57: 24000000 INFO @ Thu, 09 Dec 2021 02:28:01: 28000000 INFO @ Thu, 09 Dec 2021 02:28:02: 16000000 INFO @ Thu, 09 Dec 2021 02:28:02: 25000000 INFO @ Thu, 09 Dec 2021 02:28:06: 29000000 INFO @ Thu, 09 Dec 2021 02:28:07: 26000000 INFO @ Thu, 09 Dec 2021 02:28:07: 17000000 INFO @ Thu, 09 Dec 2021 02:28:11: 27000000 INFO @ Thu, 09 Dec 2021 02:28:11: 30000000 INFO @ Thu, 09 Dec 2021 02:28:12: 18000000 INFO @ Thu, 09 Dec 2021 02:28:16: 28000000 INFO @ Thu, 09 Dec 2021 02:28:16: 31000000 INFO @ Thu, 09 Dec 2021 02:28:17: 19000000 INFO @ Thu, 09 Dec 2021 02:28:20: 29000000 INFO @ Thu, 09 Dec 2021 02:28:22: 32000000 INFO @ Thu, 09 Dec 2021 02:28:23: 20000000 INFO @ Thu, 09 Dec 2021 02:28:25: 30000000 INFO @ Thu, 09 Dec 2021 02:28:27: 33000000 INFO @ Thu, 09 Dec 2021 02:28:28: 21000000 INFO @ Thu, 09 Dec 2021 02:28:29: 31000000 INFO @ Thu, 09 Dec 2021 02:28:32: 34000000 INFO @ Thu, 09 Dec 2021 02:28:33: 22000000 INFO @ Thu, 09 Dec 2021 02:28:34: 32000000 INFO @ Thu, 09 Dec 2021 02:28:37: 35000000 INFO @ Thu, 09 Dec 2021 02:28:38: 23000000 INFO @ Thu, 09 Dec 2021 02:28:39: 33000000 INFO @ Thu, 09 Dec 2021 02:28:43: 36000000 INFO @ Thu, 09 Dec 2021 02:28:43: 24000000 INFO @ Thu, 09 Dec 2021 02:28:44: 34000000 INFO @ Thu, 09 Dec 2021 02:28:45: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:28:45: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:28:45: #1 total tags in treatment: 36465726 INFO @ Thu, 09 Dec 2021 02:28:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:28:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:28:46: #1 tags after filtering in treatment: 36465726 INFO @ Thu, 09 Dec 2021 02:28:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:28:46: #1 finished! INFO @ Thu, 09 Dec 2021 02:28:46: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:28:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:28:48: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:28:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:28:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Thu, 09 Dec 2021 02:28:48: 35000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:28:49: 25000000 INFO @ Thu, 09 Dec 2021 02:28:53: 36000000 INFO @ Thu, 09 Dec 2021 02:28:54: 26000000 INFO @ Thu, 09 Dec 2021 02:28:55: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:28:55: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:28:55: #1 total tags in treatment: 36465726 INFO @ Thu, 09 Dec 2021 02:28:55: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:28:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:28:56: #1 tags after filtering in treatment: 36465726 INFO @ Thu, 09 Dec 2021 02:28:56: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:28:56: #1 finished! INFO @ Thu, 09 Dec 2021 02:28:56: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:28:56: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:28:58: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:28:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:28:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:28:59: 27000000 INFO @ Thu, 09 Dec 2021 02:29:04: 28000000 INFO @ Thu, 09 Dec 2021 02:29:09: 29000000 INFO @ Thu, 09 Dec 2021 02:29:14: 30000000 INFO @ Thu, 09 Dec 2021 02:29:19: 31000000 INFO @ Thu, 09 Dec 2021 02:29:24: 32000000 INFO @ Thu, 09 Dec 2021 02:29:29: 33000000 INFO @ Thu, 09 Dec 2021 02:29:35: 34000000 INFO @ Thu, 09 Dec 2021 02:29:40: 35000000 INFO @ Thu, 09 Dec 2021 02:29:45: 36000000 INFO @ Thu, 09 Dec 2021 02:29:48: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:29:48: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:29:48: #1 total tags in treatment: 36465726 INFO @ Thu, 09 Dec 2021 02:29:48: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:29:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:29:48: #1 tags after filtering in treatment: 36465726 INFO @ Thu, 09 Dec 2021 02:29:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:29:48: #1 finished! INFO @ Thu, 09 Dec 2021 02:29:48: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:29:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:29:50: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:29:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:29:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582551/SRX8582551.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。