Job ID = 14160391 SRX = SRX8582550 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 58071124 spots for SRR12054111/SRR12054111.sra Written 58071124 spots for SRR12054111/SRR12054111.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160546 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:57 58071124 reads; of these: 58071124 (100.00%) were unpaired; of these: 522885 (0.90%) aligned 0 times 47240662 (81.35%) aligned exactly 1 time 10307577 (17.75%) aligned >1 times 99.10% overall alignment rate Time searching: 00:13:57 Overall time: 00:13:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 16733798 / 57548239 = 0.2908 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:30:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:30:22: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:30:22: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:30:27: 1000000 INFO @ Thu, 09 Dec 2021 02:30:32: 2000000 INFO @ Thu, 09 Dec 2021 02:30:37: 3000000 INFO @ Thu, 09 Dec 2021 02:30:42: 4000000 INFO @ Thu, 09 Dec 2021 02:30:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:30:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:30:51: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:30:51: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:30:52: 6000000 INFO @ Thu, 09 Dec 2021 02:30:57: 1000000 INFO @ Thu, 09 Dec 2021 02:30:58: 7000000 INFO @ Thu, 09 Dec 2021 02:31:03: 2000000 INFO @ Thu, 09 Dec 2021 02:31:03: 8000000 INFO @ Thu, 09 Dec 2021 02:31:08: 3000000 INFO @ Thu, 09 Dec 2021 02:31:09: 9000000 INFO @ Thu, 09 Dec 2021 02:31:14: 4000000 INFO @ Thu, 09 Dec 2021 02:31:14: 10000000 INFO @ Thu, 09 Dec 2021 02:31:19: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:31:20: 11000000 INFO @ Thu, 09 Dec 2021 02:31:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:31:22: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:31:22: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:31:25: 6000000 INFO @ Thu, 09 Dec 2021 02:31:25: 12000000 INFO @ Thu, 09 Dec 2021 02:31:29: 1000000 INFO @ Thu, 09 Dec 2021 02:31:31: 13000000 INFO @ Thu, 09 Dec 2021 02:31:31: 7000000 INFO @ Thu, 09 Dec 2021 02:31:36: 2000000 INFO @ Thu, 09 Dec 2021 02:31:37: 14000000 INFO @ Thu, 09 Dec 2021 02:31:37: 8000000 INFO @ Thu, 09 Dec 2021 02:31:43: 3000000 INFO @ Thu, 09 Dec 2021 02:31:43: 15000000 INFO @ Thu, 09 Dec 2021 02:31:43: 9000000 INFO @ Thu, 09 Dec 2021 02:31:49: 16000000 INFO @ Thu, 09 Dec 2021 02:31:49: 4000000 INFO @ Thu, 09 Dec 2021 02:31:49: 10000000 INFO @ Thu, 09 Dec 2021 02:31:55: 17000000 INFO @ Thu, 09 Dec 2021 02:31:55: 11000000 INFO @ Thu, 09 Dec 2021 02:31:56: 5000000 INFO @ Thu, 09 Dec 2021 02:32:01: 18000000 INFO @ Thu, 09 Dec 2021 02:32:01: 12000000 INFO @ Thu, 09 Dec 2021 02:32:02: 6000000 INFO @ Thu, 09 Dec 2021 02:32:07: 19000000 INFO @ Thu, 09 Dec 2021 02:32:07: 13000000 INFO @ Thu, 09 Dec 2021 02:32:09: 7000000 INFO @ Thu, 09 Dec 2021 02:32:13: 14000000 INFO @ Thu, 09 Dec 2021 02:32:13: 20000000 INFO @ Thu, 09 Dec 2021 02:32:16: 8000000 INFO @ Thu, 09 Dec 2021 02:32:19: 21000000 INFO @ Thu, 09 Dec 2021 02:32:19: 15000000 INFO @ Thu, 09 Dec 2021 02:32:23: 9000000 INFO @ Thu, 09 Dec 2021 02:32:25: 22000000 INFO @ Thu, 09 Dec 2021 02:32:25: 16000000 INFO @ Thu, 09 Dec 2021 02:32:29: 10000000 INFO @ Thu, 09 Dec 2021 02:32:31: 23000000 INFO @ Thu, 09 Dec 2021 02:32:31: 17000000 INFO @ Thu, 09 Dec 2021 02:32:36: 11000000 INFO @ Thu, 09 Dec 2021 02:32:37: 24000000 INFO @ Thu, 09 Dec 2021 02:32:37: 18000000 INFO @ Thu, 09 Dec 2021 02:32:42: 12000000 INFO @ Thu, 09 Dec 2021 02:32:43: 25000000 INFO @ Thu, 09 Dec 2021 02:32:43: 19000000 INFO @ Thu, 09 Dec 2021 02:32:48: 13000000 INFO @ Thu, 09 Dec 2021 02:32:48: 26000000 INFO @ Thu, 09 Dec 2021 02:32:49: 20000000 INFO @ Thu, 09 Dec 2021 02:32:55: 27000000 INFO @ Thu, 09 Dec 2021 02:32:55: 14000000 INFO @ Thu, 09 Dec 2021 02:32:55: 21000000 INFO @ Thu, 09 Dec 2021 02:33:01: 28000000 INFO @ Thu, 09 Dec 2021 02:33:01: 22000000 INFO @ Thu, 09 Dec 2021 02:33:01: 15000000 INFO @ Thu, 09 Dec 2021 02:33:07: 29000000 INFO @ Thu, 09 Dec 2021 02:33:07: 23000000 INFO @ Thu, 09 Dec 2021 02:33:08: 16000000 INFO @ Thu, 09 Dec 2021 02:33:13: 30000000 INFO @ Thu, 09 Dec 2021 02:33:13: 24000000 INFO @ Thu, 09 Dec 2021 02:33:15: 17000000 INFO @ Thu, 09 Dec 2021 02:33:18: 31000000 INFO @ Thu, 09 Dec 2021 02:33:19: 25000000 INFO @ Thu, 09 Dec 2021 02:33:21: 18000000 INFO @ Thu, 09 Dec 2021 02:33:24: 32000000 INFO @ Thu, 09 Dec 2021 02:33:24: 26000000 INFO @ Thu, 09 Dec 2021 02:33:28: 19000000 INFO @ Thu, 09 Dec 2021 02:33:30: 33000000 INFO @ Thu, 09 Dec 2021 02:33:30: 27000000 INFO @ Thu, 09 Dec 2021 02:33:34: 20000000 INFO @ Thu, 09 Dec 2021 02:33:36: 34000000 INFO @ Thu, 09 Dec 2021 02:33:36: 28000000 INFO @ Thu, 09 Dec 2021 02:33:41: 21000000 INFO @ Thu, 09 Dec 2021 02:33:42: 35000000 INFO @ Thu, 09 Dec 2021 02:33:42: 29000000 INFO @ Thu, 09 Dec 2021 02:33:47: 22000000 INFO @ Thu, 09 Dec 2021 02:33:48: 36000000 INFO @ Thu, 09 Dec 2021 02:33:48: 30000000 INFO @ Thu, 09 Dec 2021 02:33:54: 23000000 INFO @ Thu, 09 Dec 2021 02:33:54: 37000000 INFO @ Thu, 09 Dec 2021 02:33:54: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:33:59: 38000000 INFO @ Thu, 09 Dec 2021 02:34:00: 32000000 INFO @ Thu, 09 Dec 2021 02:34:00: 24000000 INFO @ Thu, 09 Dec 2021 02:34:05: 39000000 INFO @ Thu, 09 Dec 2021 02:34:06: 33000000 INFO @ Thu, 09 Dec 2021 02:34:06: 25000000 INFO @ Thu, 09 Dec 2021 02:34:11: 40000000 INFO @ Thu, 09 Dec 2021 02:34:12: 34000000 INFO @ Thu, 09 Dec 2021 02:34:13: 26000000 INFO @ Thu, 09 Dec 2021 02:34:16: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:34:16: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:34:16: #1 total tags in treatment: 40814441 INFO @ Thu, 09 Dec 2021 02:34:16: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:34:17: #1 tags after filtering in treatment: 40814441 INFO @ Thu, 09 Dec 2021 02:34:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:34:17: #1 finished! INFO @ Thu, 09 Dec 2021 02:34:17: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:34:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:34:18: 35000000 INFO @ Thu, 09 Dec 2021 02:34:19: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:34:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:34:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:34:19: 27000000 INFO @ Thu, 09 Dec 2021 02:34:23: 36000000 INFO @ Thu, 09 Dec 2021 02:34:26: 28000000 INFO @ Thu, 09 Dec 2021 02:34:29: 37000000 INFO @ Thu, 09 Dec 2021 02:34:32: 29000000 INFO @ Thu, 09 Dec 2021 02:34:35: 38000000 INFO @ Thu, 09 Dec 2021 02:34:39: 30000000 INFO @ Thu, 09 Dec 2021 02:34:41: 39000000 INFO @ Thu, 09 Dec 2021 02:34:45: 31000000 INFO @ Thu, 09 Dec 2021 02:34:47: 40000000 INFO @ Thu, 09 Dec 2021 02:34:51: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:34:51: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:34:51: #1 total tags in treatment: 40814441 INFO @ Thu, 09 Dec 2021 02:34:51: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:34:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:34:52: 32000000 INFO @ Thu, 09 Dec 2021 02:34:52: #1 tags after filtering in treatment: 40814441 INFO @ Thu, 09 Dec 2021 02:34:52: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:34:52: #1 finished! INFO @ Thu, 09 Dec 2021 02:34:52: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:34:52: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:34:55: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:34:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:34:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:34:58: 33000000 INFO @ Thu, 09 Dec 2021 02:35:04: 34000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:35:10: 35000000 INFO @ Thu, 09 Dec 2021 02:35:16: 36000000 INFO @ Thu, 09 Dec 2021 02:35:22: 37000000 INFO @ Thu, 09 Dec 2021 02:35:28: 38000000 INFO @ Thu, 09 Dec 2021 02:35:34: 39000000 INFO @ Thu, 09 Dec 2021 02:35:40: 40000000 INFO @ Thu, 09 Dec 2021 02:35:45: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 02:35:45: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 02:35:45: #1 total tags in treatment: 40814441 INFO @ Thu, 09 Dec 2021 02:35:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:35:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:35:46: #1 tags after filtering in treatment: 40814441 INFO @ Thu, 09 Dec 2021 02:35:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:35:46: #1 finished! INFO @ Thu, 09 Dec 2021 02:35:46: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:35:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:35:48: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 02:35:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 02:35:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX8582550/SRX8582550.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling